Regenerative potential of cultured gingival fibroblasts in treatment of periodontal intrabony defects (randomized clinical and biochemical trial)

Abstract

Background: Defective cellular elements constitute an important challenge to achieve predictable periodontal regeneration. In an attempt to improve the cellularity of periodontal defects, gingival fibroblasts were implanted without their associated extracellular elements in periodontal defects to expose them to periodontal tissue mediators. In order to investigate the regenerative potential of gingival fibroblasts translocated into periodontal defects, the present study was designed to clinically and biochemically investigate the use of gingival fibroblasts (GF) and their associated mesenchymal stem cells (GMSC) in the treatment of intrabony periodontal defects. Methods:A total of 20 subjects were randomly divided into two groups (n = 20).Group I: ten patients were included with ten intrabony periodontal defects that received β-calcium triphosphate (β-TCP) followed by collagen membrane defect coverage, while group II: (10 patients) ten periodontal defects received cultured gingival fibroblasts (GF) on the β-TCP scaffold and covered by a collagen membrane. The clinical evaluation was carried out at the beginning and at 6 months. Gingival crevicular fluid (GCF) samples were collected directly from the test sites for the quantitative measurement of PDGF-BB and BMP-2 using the ELISA kit at 1, 7, 14, and 21 days after surgery.

Osteogenic differentiation of human mesenchymal stromal cells and fibroblasts differs depending on tissue origin and replicative senescence

“…Nevertheless, initial and promising approaches were made to evaluate FB therapeutic potential. Therefore, gingival fibroblasts have been used for the treatment of periodontal intrabony defects 77 , and a multicenter study confirmed the usefulness of FB in treating chronic foot ulcers 78 .…”

Gingiva-Derived Mesenchymal Stem Cells: Potential Application in Tissue Engineering and Regenerative Medicine - A Comprehensive Review

“…In addition, Fawzy El-Sayed KM et al have demonstrated that implantation of autologous GMSCs in conjunction with IL-1raloaded/A-sECM in the premolar/molar area significantly facilitated periodontal regeneration in a periodontal defect model in miniature pigs (105). Most interestingly, Abdal-Wahab M et al have recently performed a randomized clinical trial study to explore the potential application of gingival fibroblasts (GF) and their associated mesenchymal stem cells (GMSC) in the treatment of intrabony periodontal defects (106).…”

Dental-Derived Mesenchymal Stem Cells: State of the Art

“…In the dental research field, researchers also give attention to the regeneration of periodontal defects with DMSCs. GMSCs in combination with β-calcium triphosphate (β-TCP) scaffold could successfully reduce the vertical pocket depth and improve the cellularity of regenerated periodontal tissues (Abdal-Wahab et al, 2020). However, another similar study by using PDLSCs and a xenogeneic bone substitute (XBS) demonstrated no statistically significant differences concerning healing and re-ossification whether PDLSCs were present or not (Sánchez et al, 2020), which suggests that we should interpret experimental results with extra caution when elucidating the precise functions and effects of DMSCs.…”