Regenerative medicine using stem cells from human exfoliated deciduous teeth (SHED): a promising new treatment in pediatric surgery

Taguchi, Tomoaki; Yanagi, Yusuke; Yoshimaru, Koichiro; Zhang, Xiu Ying; Matsuura, Toshiharu; Nakayama, Koichi; Kobayashi, Eiji; Yamaza, Haruyoshi; Nonaka, Kenichi; Ohga, Shouichi; Yamaza, Takayoshi

doi:10.1007/s00595-019-01783-z

Cited by 37 publications

(38 citation statements)

References 15 publications

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“…Others studies have also evaluated the potential of SHED and its performance in tissue engineering aimed at bone regeneration and cell therapy for the treatment of systemic diseases such as hypoxic-ischemic brain injury, systemic lupus erythematosus, ulcerative colitis, spinal cord injury, Parkinson disease, and diabetes [Yamaza et al, 2010;Yamagata et al, 2013;Sonoda et al, 2015;Taguchi et al, 2019]. Sakai et al [2010] performed in vivo and in vitro studies to investigate the differentiation potential of SHED in functional odontoblasts [Sakai et al, 2010].…”

Section: Current Applicationsmentioning

confidence: 99%

“…One of the greatest challenges, currently, is to find safe and proper sources of stem cells both for research and therapeutic use [West et al, 2014]. Stem cells of human exfoliated deciduous teeth (SHED) are stem cells which have a minimal risk of oncogenesis, have a high prolif-erative and immunosuppressive capacity, and also high multipotency [Taguchi et al, 2019]. Also, SHED can be easily obtained since a child has 20 deciduous teeth that can be used as a source [Miura et al, 2003;d'Aquino et al, 2009;Koyama et al, 2009;Volponi et al, 2010;Ginani et al, 2016;Saez et al, 2016].…”

Section: Introductionmentioning

confidence: 99%

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A Biobank of Stem Cells of Human Exfoliated Deciduous Teeth: Overview of Applications and Developments in Brazil

Zalaf

Bringel

Jorge

et al. 2020

Cells Tissues Organs

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A biobank is an organized collection of biological human material and its associated information stored for research according to regulations under institutional responsibility, without commercial purposes, being a mandatory and strategical activity for research, regenerative medicine, and innovation. Stem cells have largely been employed in research and frequently stored in biobanks, which have been used as an essential source of biological materials. Stem cells of human exfoliated deciduous teeth (SHED) are stem cells which have a high multipotency and can be easily obtained. Besides, this extremely accessible tissue has advantages with respect to storage, as the SHED obtained in childhood can be used in later life, which implies the necessity for the creation and regulation of biobanks. The proper planning for the creation of a biobank includes knowledge of the material types to be stored, requirements regarding handling and storage conditions, storage time, and room for the number of samples. Thus, this study aimed to establish an overview of the development of a SHED biobank. Ethical and legal standardization, current applications, specific orientations, and challenges for the implementation of a SHED biobank were discussed. Through this overview, we hope to encourage further studies to use SHED biobanks.

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Section: Current Applicationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Biobank of Stem Cells of Human Exfoliated Deciduous Teeth: Overview of Applications and Developments in Brazil

Zalaf

Bringel

Jorge

et al. 2020

Cells Tissues Organs

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“…Of these, SHEDs, which have exfoliative characteristics, are the most easily obtained odontogenic tissue, via a little or noninvasive procedure. The pulp tissue can be obtained during the period of the changing of the child's teeth, between 5 and 12 years of age, with insignificant ethical implications and provides a suitable alternative for pediatric regenerative medicine (Taguchi et al, 2019). SHEDs display high proliferative capacity, multi-lineage differentiation, secretion of immunomodulatory molecules.…”

Section: Third Generation Of Palatal Reconstruction: Bone Bioengineeringmentioning

confidence: 99%

Extracellular Matrix Composition and Remodeling: Current Perspectives on Secondary Palate Formation, Cleft Lip/Palate, and Palatal Reconstruction

Paiva

Maas

Santos

et al. 2019

Front. Cell Dev. Biol.

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Craniofacial development comprises a complex process in humans in which failures or disturbances frequently lead to congenital anomalies. Cleft lip with/without palate (CL/P) is a common congenital anomaly that occurs due to variations in craniofacial development genes, and may occur as part of a syndrome, or more commonly in isolated forms (non-syndromic). The etiology of CL/P is multifactorial with genes, environmental factors, and their potential interactions contributing to the condition. Rehabilitation of CL/P patients requires a multidisciplinary team to perform the multiple surgical, dental, and psychological interventions required throughout the patient's life. Despite progress, lip/palatal reconstruction is still a major treatment challenge. Genetic mutations and polymorphisms in several genes, including extracellular matrix (ECM) genes, soluble factors, and enzymes responsible for ECM remodeling (e.g., metalloproteinases), have been suggested to play a role in the etiology of CL/P; hence, these may be considered likely targets for the development of new preventive and/or therapeutic strategies. In this context, investigations are being conducted on new therapeutic approaches based on tissue bioengineering, associating stem cells with biomaterials, signaling molecules, and innovative technologies. In this review, we discuss the role of genes involved in ECM composition and remodeling during secondary palate formation and pathogenesis and genetic etiology of CL/P. We also discuss potential therapeutic approaches using bioactive molecules and principles of tissue bioengineering for state-of-the-art CL/P repair and palatal reconstruction.

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“…SHED also exhibit improved properties including colony formation, proliferation, and immunomodulatory function, and are less tumorigenic than bone marrow mesenchymal stem cells (BMMSCs) [3,4]. Human clinical trials aimed at treating traumainduced tooth injury [5] as well as animal pre-clinical studies for diseases including systemic lupus erythematosus (SLE), spinal injury, and Wilson's disease [2,3,6,7] have reported that SHED are promising candidates for cell-based therapies [8]. Systemic SHED transplantation-based therapy employs multiple mechanisms, including direct conversion into target tissue-speci c cells, release of trophic factors, and cell-cell contact [3,6,7,[9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Extracellular vesicles from deciduous pulp stem cells recover bone loss by regulating telomerase activity in an osteoporosis mouse model

Sonoda

Murata

Nishida

et al. 2020

Preprint

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Background: Systemic transplantation of stem cells from human exfoliated deciduous teeth (SHED) recover bone loss in animal models of osteoporosis; however, the mechanisms underlying this remain unclear. Here, we hypothesized that trophic factors within SHED-releasing extracellular vesicles (SHED-EVs) rescue osteoporotic phenotype. Methods: EVs were isolated from culture supernatant of SHED. SHED-EVs were treated with or without ribonuclease and systemically administrated into ovariectomized mice, followed by the function of recipient bone marrow mesenchymal stem cells (BMMSCs) including telomerase activity, osteoblast differentiation, and sepmaphorine-3A (SEMA3A) secretion. Subsequently, human BMMSCs were stimulated by SHED-EVs with or without ribonuclease treatment, and then human BMMSCs were examined the function of telomerase activity, osteoblast differentiation, and SEMA3A secretion. Furthermore, SHED-EVs treated human BMMSCs were subcutaneously transplanted into dorsal skin of immunocompromised mice with hydroxyapatite tricalcium phosphate (HA/TCP) careers and analyzed the de novo bone forming ability.Results: We revealed that systemic SHED-EV-infusion recovered bone volume in ovariectomized mice and improved the function of recipient BMMSCs by rescuing the mRNA levels of Tert and telomerase activity, osteoblast differentiation, and SEMA3A secretion. Ribonuclease treatment depleted RNAs, including microRNAs, within SHED-EVs and these RNA-depleted SHED-EVs attenuated SHED-EV-rescued function of recipient BMMSCs in the ovariectomized mice. These findings were supported by in vitro assays using human BMMSCs incubated with SHED-EVs. Conclusion: Collectively, our findings suggest that SHED-secreted RNAs, such as microRNAs, play a crucial role in treating postmenopausal osteoporosis by targeting the telomerase activity of recipient BMMSCs.

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Regenerative medicine using stem cells from human exfoliated deciduous teeth (SHED): a promising new treatment in pediatric surgery

Cited by 37 publications

References 15 publications

A Biobank of Stem Cells of Human Exfoliated Deciduous Teeth: Overview of Applications and Developments in Brazil

A Biobank of Stem Cells of Human Exfoliated Deciduous Teeth: Overview of Applications and Developments in Brazil

Extracellular Matrix Composition and Remodeling: Current Perspectives on Secondary Palate Formation, Cleft Lip/Palate, and Palatal Reconstruction

Extracellular vesicles from deciduous pulp stem cells recover bone loss by regulating telomerase activity in an osteoporosis mouse model

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