Regenerative medicine: Current therapies and future directions

Mao, Angelo S.; Mooney, David J.

doi:10.1073/pnas.1508520112

Cited by 701 publications

(492 citation statements)

References 140 publications

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“…Finally, the adult liver also harbors regenerative capacity; in mammals, pre-existing multipotent stem cells are found at various anatomical reservoirs such as adipose tissue and bone marrow. Tissue engineering studies of bioscaffolds loaded with immature cell populations have revealed that precursor and stem cells bear the potential of repopulating whole organs (3,4). Moreover, emerging methodologies such as the advent of induced pluripotent cells (iPSC) where a cocktail of transcription factors ( Yamanaka factors ) can be used to reprogram adult cell populations into distinct cell lineages are being intensively pursued.…”

Section: Amphibian and Mammalian Regeneration: The Importance Of Oxygenmentioning

confidence: 99%

Oxygen, Metabolism, and Regeneration: Lessons from Mice

Heber‐Katz

2017

Trends in Molecular Medicine

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The discovery that the Murphy Roths Large (MRL) mouse strain is a fully competent, epimorphic tissue regenerator, proved that the machinery of regeneration was preserved through evolution from hydra, to salamanders, to mammals. Such concepts have allowed translation of the biology of amphibians -- and their ability to regenerate -- to a mammalian context. In particular, the ancient hypoxia inducible factor (HIF-1α) pathway, operating through prolyl hydroxylase domain proteins (PHDs), was identified as a central player in mouse regeneration. Thus, the possibility of targeting PHDs or other HIF-1α modifiers to effectively recreate the amphibian regenerative state has emerged. We posit that these regenerative pathways are critical in mammals. Moreover, the current approved use of PHD inhibitors in the clinic should allow fast-track translation from mouse studies to drug-based regenerative therapy in humans.

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Section: Amphibian and Mammalian Regeneration: The Importance Of Oxygenmentioning

confidence: 99%

Oxygen, Metabolism, and Regeneration: Lessons from Mice

Heber‐Katz

2017

Trends in Molecular Medicine

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“…The appropriate candidates for cell therapy and regenerative medicine are stem cells (1)(2)(3). Three main characteristics of stem cells are high proliferation, self-renewal, and differentiation (4).…”

Section: Introductionmentioning

confidence: 99%

Effects of Epidermal Growth Factor, Glial Cell Line-Derived Neurotrophic and Leukemia Inhibitory Factor on the Proliferation and Differentiation Potential of Adipose Tissue-Derived Mesenchymal Stem Cells

Dashtaki¹,

Nezhad²,

Hemadi³

et al. 2017

Iran Red Crescent Med J

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Background: There is a great deal of interest in using adipose tissue-derived mesenchymal stem cells (AT-MSCs) for clinical applications. However, the important limitations of clinical application of stem cells are the small number of cells and their differentiation into undesirable lineage in vitro. To overcome this problem, various growth factors are studied extensively. Objectives: The current study aimed at using 3 different doses of epidermal growth factor (EGF), glial cell line-derived neurotrophic (GDNF), and leukemia inhibitory factor (LIF) to culture AT-MSCs and evaluating their effects on proliferation, viability, differentiation potential, and maintenance of the stemness state of cells. Methods: The current experimental study was conducted on 8 -10 male NMRI (Naval medical research institute) mice provided from research center and experimental animal house of Jundishapur University of Ahvaz, Iran, from September 2016 to April 2017. AT-MSCs were isolated from mice adipose tissue. The cells were cultured with three different doses of EGF, LIF, and GDNF. The morphology and cell proliferation of the AT-MSCs were studied on the days 5, 7, and 11 by an inverted microscope and MTT assay, respectively. To evaluate the stemness state of the cells, Oct4 expression was measured using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Also, differentiation potential of AT-MSCs toward adipogenic and osteogenic lineages was assessed. All tests were done in triplicate.

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“…In one sense regenerative medicine refers to the highly technical specialty that includes transplantation of cells or biomaterials, tissue precursors (stem cells), or fully formed organs to recover lost structure and function 5. The transplantation of corneas is an example of this sense of regeneration.…”

Section: Cultivating Conditions For Rebuildingmentioning

confidence: 99%