Regenerative Medicine Approaches for Age-Related Muscle Loss and Sarcopenia: A Mini-Review

Naranjo, Juan Diego; Dziki, Jenna L.; Badylak, Stephen F.

doi:10.1159/000479278

Cited by 40 publications

(30 citation statements)

References 76 publications

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“…Muscle mass and strength are also linked to testosterone levels. An increase in age correlates to decreased testosterone levels in both men and women, which is linked to a decrease in muscle mass, particularly in men (43).…”

Section: Discussionmentioning

confidence: 99%

Force-Velocity Relationship in Three Different Variations of Prone Row Exercises

Loturco

Suchomel

Kobal³

et al. 2021

Journal of Strength and Conditioning Research

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This study examined the force-velocity relationship and tested the possibility of determining the relative loading intensity (% 1RM) in three different variations of prone row exercises. Thirty male top-level athletes from two different sports (National Team rugby union players and professional mixed martial arts fighters) were submitted to maximum dynamic strength assessments in the free prone bench pull, bent over barbell row, and bent over Smith-machine row, following standard procedures encompassing lifts performed from 40 to 100% of 1RM. The mean velocity, mean propulsive velocity, and peak velocity were measured in all attempts. Linear regression analyses were performed to establish the relationships between the different measures of bar-velocities and %1RM. The actual (obtained during the assessments) and predicted 1RM values (based on the predictive equations) for each exercise were compared using a paired t-test. In all exercises, the predicted 1RM scores - based on all velocity variables- were not different from their respective actual values. The close linear relationships between bar-velocities and distinct %1RM (coefficient of determination ≥ 80%, in all experimental conditions) allow precise determination of relative load and maximum dynamic strength, and enable coaches and sports scientists to use the different velocity outputs to rapidly and accurately monitor their athletes on a daily basis.

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Section: Discussionmentioning

confidence: 99%

Force-Velocity Relationship in Three Different Variations of Prone Row Exercises

Loturco

Suchomel

Kobal³

et al. 2021

Journal of Strength and Conditioning Research

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“…Nevertheless, the possible causal relationship of satellite cell loss of function to sarcopenia does not negate the potential of satellite cells as an intervention to restore sarcopenic muscle . The use of healthy muscle stem cells to generate new muscle fibers, and/or to contribute new myonuclei to existing fibers, can be envisioned as a viable strategy to correct the defects that drive age‐related muscle wasting, restoring muscle mass and force, and rejuvenating the sarcopenic muscle.…”

Section: Stem Cell‐based Interventions In Muscle Agingmentioning

confidence: 99%

“…However, there are no published reports of direct delivery of muscle progenitors into the muscles of old sarcopenic mice. Moreover, while these studies are promising, they have also brought to light several limitations with important implications for the use of cell therapy applied in aged skeletal muscle . Successful engraftment with the current methodology used in preclinical settings to deliver progenitor cells to the muscle requires a concurrent injury, which is not a viable strategy for the treatment of sarcopenic muscles.…”

Section: Stem Cell‐based Interventions In Muscle Agingmentioning

confidence: 99%

Understanding muscle regenerative decline with aging: new approaches to bring back youthfulness to aged stem cells

2020

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Aging is characterized by the progressive dysfunction of most tissues and organs, which has been linked to the regenerative decline of their resident stem cells over time. Skeletal muscle provides a stark example of this decline. Its stem cells, also called satellite cells, sustain muscle regeneration throughout life, but at advanced age they fail for largely undefined reasons.Here, we discuss current understanding of the molecular processes regulating satellite cell maintenance throughout life and how age-related failure of these processes contributes to muscle aging. We also highlight the emerging field of rejuvenating biology to restore features of youthfulness in satellite cells, with the ultimate goal of slowing down or reversing the age-related decline in muscle regeneration.

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“…1a and e). Fibroblasts were derived from arm and abdomen skin biopsies (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) years for the young control, n=3, and 60-70 years for the aged group, n= 3), while endothelial cells were extracted from iliac vein and artery (15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25) years for the young control, n=3, and 45-50 years for the aged group, n=3). We utilized a non-integrative reprogramming protocol that we optimized, based on a cocktail of mRNAs expressing OCT4, SOX2, KLF4, c-MYC, LIN28 and NANOG (OSKMLN) 11 .…”

Section: Mainmentioning

confidence: 99%

“…Sarcopenia is an age-related condition that is characterized by loss of muscle mass and force production 32,33 . Similarly, in mice muscle functions show progressive degeneration with age 34,35 .We wanted to test whether transient reprogramming of aged MuSCs would improve a cell-based treatment in restoring physiological functions of muscle of older mice.…”

Section: Mainmentioning

confidence: 99%

Transient non-integrative nuclear reprogramming promotes multifaceted reversal of aging in human cells

Quarta

Mukherjee

et al. 2019

Preprint

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Aging is characterized by a gradual loss of function occurring at the molecular, cellular, tissue and organismal levels [1][2][3] . At the chromatin level, aging is associated with the progressive accumulation of epigenetic errors that eventually lead to aberrant gene regulation, stem cell exhaustion, senescence, and deregulated cell/tissue homeostasis 3 . The technology of nuclear reprogramming to pluripotency, through over-expression of a small number of transcription factors, can revert both the age and the identity of any cell to that of an embryonic cell by driving epigenetic reprogramming 2,4,5 . Recent evidence has shown that transient transgenic reprogramming can ameliorate age-associated hallmarks and extend lifespan in progeroid mice 6 .However, it is unknown how this form of 'epigenetic rejuvenation' would apply to physiologically aged cells and, importantly, how it might translate to human cells. Here we show that transient reprogramming, mediated by transient expression of mRNAs, promotes a rapid reversal of both cellular aging and of epigenetic clock in human fibroblasts and endothelial cells, reduces the inflammatory profile in human chondrocytes, and restores youthful regenerative response to aged, human muscle stem cells, in each case without abolishing cellular identity. Our method, that we named Epigenetic Reprogramming of Aging (ERA), paves the way to a novel, potentially translatable strategy for ex vivo cell rejuvenation treatment. In addition, ERA holds promise for in vivo tissue rejuvenation therapies to reverse the physiological manifestations of aging and the risk for the development of age-related diseases.

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Regenerative Medicine Approaches for Age-Related Muscle Loss and Sarcopenia: A Mini-Review

Cited by 40 publications

References 76 publications

Force-Velocity Relationship in Three Different Variations of Prone Row Exercises

Force-Velocity Relationship in Three Different Variations of Prone Row Exercises

Understanding muscle regenerative decline with aging: new approaches to bring back youthfulness to aged stem cells

Transient non-integrative nuclear reprogramming promotes multifaceted reversal of aging in human cells

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