Regenerative and Protective Effects on Dental Tissues of a Fluoride–Silicon-Rich Toothpaste Associated with a Calcium Booster: An In Vitro Study

Abstract

Calcium boosters have been used as a supplement for fluoride toothpastes to repair the dental tissues and reduce dentin permeability. This in vitro study aimed to characterize the regenerative and protective effects of the treatment of dental tissues with a fluoride–silicon-rich toothpaste associated with a calcium booster. Bovine enamel and dentin blocks (n = 5) were obtained (4 × 4 × 6 mm). A fluoride–silicon-rich toothpaste and a calcium booster were used to brush the enamel and dentin both immediately and five days afterwards. The outcomes were then compared to those of the untreated control group. After that, the specimens were cross-sectioned. SEM was used to evaluate the micromorphology of the surface and cross-section. Energy-dispersive X-ray spectroscopy (EDS) was used to determine the elemental analyses (weight%). After treatment for 5 days with a booster/silicon-rich toothpaste, EDS analysis demonstrated that it induced a significant mineral change. It was also able to form a protective silicon-enriched mineral layer on both enamel and dentin surfaces. It was demonstrated in vitro that a fluoride–silicon-rich toothpaste associated with a calcium booster regenerates the dental tissues, remineralizing the enamel structure and occluding the dentin tubules.

Chemical and Ultrastructural Characterization of Dentin Treated with Remineralizing Dentifrices

“…A previous study has, however, reported a mineral layer formation on dentin only after 7 days of brushing evaluation using Dentalclean toothpaste [22]. Our results are in line with the previous report demonstrating formation of a remineralizing layer after brushing dentin with Dentalclean toothpaste associated with booster as soon as day 1 [13]. On the other…”

“…Comparable to a previous study [13], the EDX (Tables 2-4) elemental analysis along with the SEM and AFM images demonstrates that treatment with all dentifrices was able to modify the dentinal surface, also justifying the presence of elements such as Ca, F and P. These results lead to the rejection of the first proposed null hypothesis. Dentalclean dentifrice is regarded as a multifunctional phosphate-based dentifrice in an acidified and stabilized phosphate/fluoride complex.…”

“…TEM imaging (Figure 4) complemented the SEM and AFM results by showing deposited hydroxyapatite inside the dentin tubules at day 7. A previous report has also demonstrated the formation of mineralized areas inside dentinal tubules after 5 daily applications of Dentalclean associated with booster [13]. All reflections from the (101), (111), ( 200) and (211) planes can be identified as polycrystalline hydroxyapatite after SAED indexing [21,30].…”

“…An alternative dentifrice for dentin remineralization is Dentalclean Daily Regenerating Gel (Dentalclean, Londrina, PR, Brazil), which, according to the same manufacturer, can be associated with a regenerating booster component (Dentalclean, Londrina, PR, Brazil) (REFIX ® technology). The latter material uses the technology of calcium nanoparticles associated with phosphate salts, serving as a carrier for calcium to permeate dental tissues that may increase the efficacy of the remineralizing agents [13]. Both systems are capable of enhancing the action of 1450 ppm sodium fluoride compared with other commercial toothpastes [14,15].…”

Effectiveness of Bioactive Toothpastes against Dentin Hypersensitivity Using Evaporative and Tactile Analyses: A Randomized Clinical Trial

“…The results were even more significant in relation to the number of sensitive teeth treated with "pain elimination" after one month of use (69.3%), and only 4% of teeth achieved "no pain reduction", as shown in Table 5. In a previous study [36], it was observed that a considerable mineral change occurred following 5 days of treatment with a booster/silicon-rich toothpaste, forming a protective silicon-enriched mineral layer on both enamel and dentin surfaces. In vitro studies have shown that a fluoride-silicon-rich toothpaste combined with a calcium booster regenerates dental tissues by remineralizing the enamel structure and occluding dentin tubules [36].…”