Regeneration von haploiden und diploiden pflanzen aus protoplasten von Petunia hybrida L.

Rapid and high frequency shoot regeneration from hypocotyl protoplasts of Brassica nigra

“…The protoplast band floating on the top was collected through a pasteur pipette and washed in W 5 medium (Menczel et al 1981) by centrifugation. It was resuspended at an approximation density of 5 × 104 protoplasts/ml in the two culture media, Kao's (Kao 1977) or V47 (Binding 1974) containing growth regulators 2,4-D (1 mg 1 1), NAA (0.1 mg 1 -l) and zeatin riboside (0.5mg 1-1) and supplemented with 7.2% (w/v) glucose instead of mannitol. Viability tests were made using Evan's blue staining.…”

The Genetics of Petunia

“…After the first attempts by Potrykus (1972) using petal or ovule cells, the techniques of protoplast culture, callus proliferation, and plant regeneration were rapidly refined in Germany (Durand et al 1973;Binding 1974a) and Great Britain (Frearson et al 1973). Complementary methods were developed for the whole genus Power et al 1976b;Sink and Power 1977).…”

Protoplast isolation and plant regeneration of different genotypes of Petunia and Calibrachoa

“…Techniques for protoplast isolation and culture in Petunia have been reported (Frearson et al 1973;Binding 1974;Hayward and Power 1975;Izhar and Power 1977), but only for a few genotypes of minor or no commercial importance. These protocols are also available for wild species of Calibrachoa, including P. parviflora (Power et al 1980) and P. variabilis (Taguchi et al 1993).…”