Regeneration of vascularized bone

Hsiong, Susan X.; Mooney, David J.

doi:10.1111/j.1600-0757.2006.00158.x

Cited by 77 publications

(63 citation statements)

References 137 publications

(152 reference statements)

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“…Genes can be delivered in vivo that i) encode for the desired protein(s), or ii) encode for transcription factors that induce expression of the desired protein(s). Most approaches have focused on the delivery of genes encoding for growth factors (e.g., vascular endothelial growth factor (VEGF), bone morphogenetic protein (BMP)), in which case transfected cells act as bioreactors for localized secretion thereby conditioning the environment [60,140]. Delivery of DNA encoding for transcription factors, or active variants thereof, may provide a different functionality.…”

Section: Transgene Expression and Tissue Formationmentioning

confidence: 99%

Matrices and scaffolds for DNA delivery in tissue engineering

Laporte¹,

Shea²

2007

Advanced Drug Delivery Reviews

240

208

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Regenerative medicine aims to create functional tissue replacements, typically through creating a controlled environment that promotes and directs the differentiation of stem or progenitor cells, either endogenous or transplanted. Scaffolds serve a central role in many strategies by providing the means to control the local environment. Gene delivery from the scaffold represents a versatile approach to manipulating the local environment for directing cell function. Research at the interface of biomaterials, gene therapy, and drug delivery has identified several design parameters for the vector and the biomaterial scaffold that must be satisfied. Progress has been made towards achieving gene delivery within a tissue engineering scaffold, though the design principles for the materials and vectors that produce efficient delivery require further development. Nevertheless, these advances in obtaining transgene expression with the scaffold have created opportunities to develop greater control of either delivery or expression and to identify the best practices for promoting tissue formation. Strategies to achieve controlled localized expression within the tissue engineering scaffold will have broad application to the regeneration of many tissues, with great promise for clinical therapies.

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Section: Transgene Expression and Tissue Formationmentioning

confidence: 99%

Matrices and scaffolds for DNA delivery in tissue engineering

Laporte¹,

Shea²

2007

Advanced Drug Delivery Reviews

240

208

View full text Add to dashboard Cite

show abstract

“…Bone receives nutrients, oxygen, osteoinductive factors, and stem cells through the blood. 20 Therefore, an appropriate stem cell population for bone regeneration would also have proangiogenic effects. 20,21 Pericytes are known to maintain endothelial cell function, 22,23 and available studies have also shown that these cells secrete more angiogenic factors, such as vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2), than do ASCs.…”

mentioning

confidence: 99%

Human Perivascular Stem Cells Show Enhanced Osteogenesis and Vasculogenesis with Nel-Like Molecule I Protein

Askarinam¹,

James

Zara

et al. 2013

Tissue Engineering Part A

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An ideal mesenchymal stem cell (MSC) source for bone tissue engineering has yet to be identified. Such an MSC population would be easily harvested in abundance, with minimal morbidity and with high purity. Our laboratories have identified perivascular stem cells (PSCs) as a candidate cell source. PSCs are readily isolatable through fluorescent-activated cell sorting from adipose tissue and have been previously shown to be indistinguishable from MSCs in the phenotype and differentiation potential. PSCs consist of two distinct cell populations: (1) pericytes (CD146 + , CD34 -, and CD45 -), which surround capillaries and microvessels, and (2) adventitial cells (CD146 -, CD34 + , and CD45 -), found within the tunica adventitia of large arteries and veins. We previously demonstrated the osteogenic potential of pericytes by examining pericytes derived from the human fetal pancreas, and illustrated their in vivo trophic and angiogenic effects. In the present study, we used an intramuscular ectopic bone model to develop the translational potential of our original findings using PSCs (as a combination of pericytes and adventitial cells) from human white adipose tissue. We evaluated human PSC (hPSC)-mediated bone formation and vascularization in vivo. We also examined the effects of hPSCs when combined with the novel craniosynostosis-associated protein, Nel-like molecule I (NELL-1). Implants consisting of the demineralized bone matrix putty combined with NELL-1 (3 mg/mL), hPSC (2.5 · 10 5 cells), or hPSC + NELL-1, were inserted in the bicep femoris of SCID mice. Bone growth was evaluated using microcomputed tomography, histology, and immunohistochemistry over 4 weeks. Results demonstrated the osteogenic potential of hPSCs and the additive effect of hPSC + NELL-1 on bone formation and vasculogenesis. Comparable osteogenesis was observed with NELL-1 as compared to the more commonly used bone morphogenetic protein-2. Next, hPSCs induced greater implant vascularization than the unsorted stromal vascular fraction from patientmatched samples. Finally, we observed an additive effect on implant vascularization with hPSC + NELL-1 by histomorphometry and immunohistochemistry, accompanied by in vitro elaboration of vasculogenic growth factors. These findings hold significant implications for the cell/protein combination therapy hPSC + NELL-1 in the development of strategies for vascularized bone regeneration.

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“…Quando os biomateriais são transferidos de um organismo para outro, seja de mesma espécie ou de espécie diferente, surgem dúvidas quanto à possibilidade de ocorrência de reações imunológicas no organismo receptor, visto que componentes do tecido ósseo, tais como, colágeno, tecido adiposo ou proteínas da matriz podem estar presentes (Graham 2010). Por outro lado, há necessidade da presença da matriz óssea extracelular que sustente/conduza o processo de remodelação óssea natural, possibilitando a formação de um novo osso (HSIONG, 2006). Além disso, há relatos de que o processo de purificação do material pode resultar em fracasso desses "andaimes naturais" por diminuir a osteocondução (GHANAATI, 2014).…”

Section: Implante Alógeno Ou Homógenounclassified

Substitutos Ósseos Alógenos E Xenógenos Comparados Ao Enxerto Autógeno: Reações Biológicas

Rodolfo

Machado

Betoni-Júnior³

et al. 2017

RBM

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Palavras-chave: Enxerto Autólogo; Xenoenxerto; Aloenxerto; Reações Biológicas. Allogenous and xenogenous bone substitutes compared to autogenous bone graft: biologycal reactions AbstractThe implant supported rehabilitation of edentulous patients is hampered by insufficient bone volume, thus, requiring a prior bone reconstruction. Autogenous bone is still the "gold standard" biomaterial, which is effective in bone regeneration process, contains viable cells and doesn't transmit infectious diseases nor triggers immune responses. Moreover, it presents rapid incorporation and consolidation. However, this type of graft has disadvantages such as increased morbidity and limited availability. In view of the constant search for bone substitutes that may have properties similar to autogenous bone, but that do not require a second surgical site, the use of allogeneic (from individuals of the same species, but genetically Substitutos ósseos alógenos e xenógenos comparados... RODOLFO et al. 96 97

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Regeneration of vascularized bone

Cited by 77 publications

References 137 publications

Matrices and scaffolds for DNA delivery in tissue engineering

Matrices and scaffolds for DNA delivery in tissue engineering

Human Perivascular Stem Cells Show Enhanced Osteogenesis and Vasculogenesis with Nel-Like Molecule I Protein

Substitutos Ósseos Alógenos E Xenógenos Comparados Ao Enxerto Autógeno: Reações Biológicas

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