Regeneration of the Bladder after Subtotal Cystectomy in Rats

Liang, Daniel S.; Goss, Richard J.

doi:10.1016/s0022-5347(17)64568-3

Cited by 30 publications

(9 citation statements)

References 9 publications

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“…The rather remarkable regenerative capacity of the bladder has been recognized in rodents, canines and humans for decades [6], [17], [18], [19], [20]. This insight, coupled with the limitations and complications associated with using intestinal bowel segments has led to pursuit of new treatment options for bladder augmentation and reconstruction, and recently culminated in the development of autologous cell-seeded polymer scaffolds as an alternative to traditional cystoplasty and/or neobladder techniques [4].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Early Proliferative Response of the Rodent Bladder to Subtotal Cystectomy: A Unique Model of Mammalian Organ Regeneration

et al. 2012

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Subtotal cystectomy (STC; surgical removal of ∼75% of the rat urinary bladder) elicits a robust proliferative response resulting in complete structural and functional bladder regeneration within 8-weeks. The goal of these studies was to characterize the early cellular response that mediates this regenerative phenomenon, which is unique among mammalian organ systems. STC was performed on eighteen 12-week-old female Fischer F344 rats. At 1, 3, 5 and 7-days post-STC, the bladder was harvested 2-hours after intraperitoneal injection of bromodeoxyuridine (BrdU). Fluorescent BrdU labeling was quantified in cells within the urothelium, lamina propria (LP), muscularis propria (MP) and serosa. Cell location was confirmed with fluorescently co-labeled cytokeratin, vimentin or smooth muscle actin (SMA), to identify urothelial, interstitial and smooth muscle cells, respectively. Expression of sonic hedgehog (Shh), Gli-1 and bone morphogenic factor-4 (BMP-4) were evaluated with immunochemistry. Three non-operated rats injected with BrdU served as controls. Less than 1% of cells in the bladder wall were labeled with BrdU in control bladders, but this percentage significantly increased by 5-8-fold at all time points post-STC. The spatiotemporal characteristics of the proliferative response were defined by a significantly higher percentage of BrdU-labeled cells within the urothelium at 1-day than in the MP and LP. A time-dependent shift at 3 and 5-days post-STC revealed significantly fewer BrdU-labeled cells in the MP than LP or urothelium. By 7-days the percentage of BrdU-labeled cells was similar among urothelium, LP and MP. STC also caused an increase in immunostaining for Shh, Gli-1 and BMP-4. In summary, the early stages of functional bladder regeneration are characterized by time-dependent changes in the location of the proliferating cell population, and expression of several evolutionarily conserved developmental signaling proteins. This report extends previous observations and further establishes the rodent bladder as an excellent model for studying novel aspects of mammalian organ regeneration.

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Section: Introductionmentioning

confidence: 99%

Characterization of the Early Proliferative Response of the Rodent Bladder to Subtotal Cystectomy: A Unique Model of Mammalian Organ Regeneration

et al. 2012

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“…As mesothelial cells are known to be easily detached by mechanical or chemical methods [13], a change in the chemical or osmotic environment in the sac may induce the mesothelial cells to fall off spontaneously. Transitional epithelia are known to move rapidly to cover the lost surface when part of the epithelium is removed [14]. In our experiment, the epithelia moved as a cell sheet from the cut edge of the epithelium as early as Day 3 after operation.…”

Section: Mechanism Of Epithelializationmentioning

confidence: 77%

Use of abdominal wall with mesothelium as a substrate for reconstitution of urinary bladder wall: Replacement of the mesothelium by the urothelium and complete bladder wall reconstruction

Yoshida

Sheng

Sawada

2005

Scandinavian Journal of Urology and Nephrology

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“…In pioneering studies by Atala et al (2006), bladder neo-organs were constructed by seeding synthetic scaffolds (collagen or collagen/polyglycolic acid composites) with urothelial cells on the inside and smooth muscle cells on the outside and subsequently implanted into subjects with myelomeningocele. Numerous reports suggested that both animal (Liang and Goss, 1963;Oberpenning et al, 1999;Frederiksen et al, 2004;Burmeister et al, 2010) and human (Sisk and Neu, 1939;Liang, 1962;Tucci and Haralambidis, 1963) bladders have significant regenerative potential after removal of a large portion of the organ (subtotal cystectomy; STC).…”

Section: A Bladder Diseasementioning

confidence: 99%