Regeneration of Shoot from Cotyledon Derived Callus of Chickpea (Cicer arietinum
L.)

., S. Huda; ., N.A. Siddique; Khatun, Nazia; ., M.H. Rahman; Morshed, M. T.

doi:10.3923/pjbs.2003.1310.1313

Cited by 14 publications

(5 citation statements)

References 2 publications

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“…According to Altınkut Uncuoğlu et al (1997), the same hormones (IAA and kinetin) used at concentrations of 0.2 mg L -1 and 0.5 mg L -1 and with the addition of another cytokinin (benzyl adenine) resulted in a rate of callogenesis of 85%. Furthermore, similar results have been reported by Sagare et al (1993), Huda et al (2003), Zare Mirakabad et al (2011, Aasim et al (2011), andSani andMustapha (2010), which indicates that the combination of these two hormones is favorable to induce dedifferentiation and cell proliferation in Cicer arietinum.…”

Section: Discussionsupporting

confidence: 83%

Callogenesis in Cicer arietinum and identification of a genotype resistant to Ascochyta rabiei

2020

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The chickpea (Cicer arietinum) is one of the leguminous species most appreciated by consumers in the Mediterranean basin, while being an important source of protein. Nevertheless, its crop yields are greatly limited by several biotic and abiotic stresses, the main one being Ascochyta rabiei, the causal agent of anthracnose. As traditional breeding methods have proved to be ineffective in controlling this pathogen, resorting to biotechnological methods is necessary. Therefore, in this study, the callogenic capacity of stem and leaflet explants from three genotypes of chickpea, namely ‘FLIP 84-92 C’, ‘ILC 32-97’, and ‘ILC 263’, cultured on Murashige and Skoog (MS) medium with different hormonal balances of auxins (indole-3-acetic acid [IAA] and 2,4-dichlorophenoxyacetic acid [2,4-D]) and cytokinin (kinetin), was determined. For all the genotypes, high percentages of callogenesis were recorded in the different explants grown on an MS medium with 2 mg of both IAA and kinetin. Then, a patho-system of Cicer arietinum calluses with Ascochyta rabiei was investigated, followed by a histological assessment of this interaction. The presence of the fruiting bodies of the pathogen was revealed in the calluses of the ‘ILC 32-97’ and ‘ILC 263’ genotypes. Notably, the latter showed a high sensitivity to the pathogen, as indicated by an abundance of pycnidia in its tissues. As for the ‘FLIP 84-92 C’ genotype, the histological sections showed a total absence of inter- and intracellular fruiting bodies of the pathogen in the callus tissues. Therefore, this genotype was considered as resistant to Ascochyta rabiei.

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Section: Discussionsupporting

confidence: 83%

Callogenesis in Cicer arietinum and identification of a genotype resistant to Ascochyta rabiei

2020

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“…Callus induction was observed from epicotyls, hypocotyls, and shoot tip of chickpea in a previous study, and the highest (100%) responses were found in MS medium with 2.0 mg/l 2, 4-D and 0.5 mg/l NAA after three weeks of culture (Kaberi et al 2013). The highest 95% response of callus induction in chickpea was found on MS medium supplemented with 3.0 mg/l 2, 4-D and 3.0 mg/l BAP (Huda and Asaduzzaman 2003). 2, 4-D alone or in combination with BAP, Kn provided 100% callus induction of chickpea in previous studies (Panday and Ganopathy 1984, Anil et al 1986a, 1986b.…”

Section: Resultsmentioning

confidence: 72%

“…Additionally, shoot induction responses of all three chickpea varieties showed almost similar efficiency in the same hormonal treatments. A maximum of 40% shoot bud formation was obtained on MS medium supplemented with 2.0 mg/l BAP and 0.5 mg/l NAA with 2.50 shoots per callus (Huda and Asaduzzaman 2003). BAP (2.0-10.0 mg/l) and IAA (0.1-1.0 mg/l) were used for shoot proliferation using callus culture from hypocotyl explants of chickpea (Islam and Riazuddin 1993).…”

Section: Resultsmentioning

confidence: 99%

In vitro Regeneration of Chickpea (Cicer arietinum L.) cultivated in Bangladesh

Sarkar¹,

Sarkar²,

Akter³

et al. 2022

Plant Tissue Cult. & Biotech.

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An efficient, and reproducible in vitro regeneration protocol was developed for three varieties of chickpea (Cicer arietinum L.) named Barichhola-9, Barichhola-10, and Barichhola-11 using embryos with decapitated root tips as explants. The best response towards callus induction was obtained on MS medium supplemented with a combination of 2.0 mg/l BAP and 0.3 mg/l NAA. MS medium containing 3.0 mg/l BAP alone provided the highest number of shoots from the induced callus. MS medium with 0.5 mg/l NAA was found to be the best for root induction from in vitro regenerated shoots in all three varieties of chickpea. In vitro regenerated well-rooted plantlets were transplanted into a small pot containing a mixture of soil and cocodust for acclimatization. Regeneration responses were found to be identical for the three varieties of chickpea used in this study. The results of the present investigation will facilitate the in vitro regeneration of chicpea varieties as well as further genetic improvement of this economically valuable crop species through genetic transformation. Plant Tissue Cult. & Biotech. 32(2): 205-216, 2022 (December)

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“…Being recalcitrant in nature chickpea transformation is difficult and a robust transformation method is a prerequisite for researchers to carry out the genetic transformation studies in the crop. Although several labs have reported chickpea transformation, the limitations associated with the reproducibility of the technique ( Huda et al, 2000 ; Das Bhowmik et al, 2019 ), poor in vitro rooting ( Polowick et al, 2004 ), low transformation efficiency, regeneration capacity and non-transmission of genes to subsequent generations ( Sarmah et al, 2004 ) remain problematic. The reports available till date indicate majority of the chickpea transformation works have been carried out using Bacillus thuringiensis genes for pod borer resistance ( Das et al, 2017 ).…”

Section: Bottlenecks In Chickpea Gene Editing Applicationsmentioning

confidence: 99%

Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat

et al. 2023

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Genomics and genome editing promise enormous opportunities for crop improvement and elementary research. Precise modification in the specific targeted location of a genome has profited over the unplanned insertional events which are generally accomplished employing unadventurous means of genetic modifications. The advent of new genome editing procedures viz; zinc finger nucleases (ZFNs), homing endonucleases, transcription activator like effector nucleases (TALENs), Base Editors (BEs), and Primer Editors (PEs) enable molecular scientists to modulate gene expressions or create novel genes with high precision and efficiency. However, all these techniques are exorbitant and tedious since their prerequisites are difficult processes that necessitate protein engineering. Contrary to first generation genome modifying methods, CRISPR/Cas9 is simple to construct, and clones can hypothetically target several locations in the genome with different guide RNAs. Following the model of the application in crop with the help of the CRISPR/Cas9 module, various customized Cas9 cassettes have been cast off to advance mark discrimination and diminish random cuts. The present study discusses the progression in genome editing apparatuses, and their applications in chickpea crop development, scientific limitations, and future perspectives for biofortifying cytokinin dehydrogenase, nitrate reductase, superoxide dismutase to induce drought resistance, heat tolerance and higher yield in chickpea to encounter global climate change, hunger and nutritional threats.

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Regeneration of Shoot from Cotyledon Derived Callus of Chickpea (Cicer arietinum L.)

Cited by 14 publications

References 2 publications

Callogenesis in Cicer arietinum and identification of a genotype resistant to Ascochyta rabiei

Callogenesis in Cicer arietinum and identification of a genotype resistant to Ascochyta rabiei

In vitro Regeneration of Chickpea (Cicer arietinum L.) cultivated in Bangladesh

Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat

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