Regeneration of rice double haploids using a onestep culture procedure

Marassi, Maria Antonia; Bovo, Oscar A.; Lavia, Graciela Inés; Mroginski, L. A.

doi:10.1016/s0176-1617(11)80464-5

Cited by 6 publications

(3 citation statements)

References 15 publications

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“…Although some authors succeeded in obtaining rice DH plantlets directly sowing anthers in onestep culture media without transferring to regeneration media so as to save time and resources (Karim and Zapata 1990;Marassi et al 1993), two-step culture media protocols are widely used and described by authors (Lentini et al 1997). As optimal media composition is highly affected by the genetic backup of the plants (Talebi et al 2007) we adapted the protocol of Spanish Hispagran (Reddy et al 1985).…”

Section: Discussionmentioning

confidence: 99%

A Mediterranean japonica rice (Oryza sativa) cultivar improvement through anther culture

et al. 2013

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Certified seed producers systematically select and propagate registered varieties year after year in order to maintain their uniformity and the original registered cultivar traits. However, natural mutations, spontaneous breeding between varieties and alien grain contamination can introduce undesirable variability. NRVC 980385 is a temperate japonica rice cultivar (Oryza sativa ssp. japonica) first registered in Spain in 2002. In 2005 certification tests detected a plot differing from the original traits in terms of uniformity and height suggesting the presence of a certain heterozygosis. This material was therefore seen as an opportunity to obtain newly stabilized doubled haploid (DH) lines which could compete in the Spanish short grain seed market. In this study, an in vitro anther culture protocol is defined which also covers the field tests selection to obtain four new, improved and stabilized DH derived lines ready to be registered for commercial proposes. This took just four years from the initial anther collection until new lines were grown in large scale field trials. Consequently, this protocol reduces the time for obtaining field assessed DH lines thereby having considerable advantages over other techniques by both maintaining the original registered cultivars and/or generating new derived varieties.

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Section: Discussionmentioning

confidence: 99%

A Mediterranean japonica rice (Oryza sativa) cultivar improvement through anther culture

et al. 2013

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“…Several studies have focused on the improvement of culture conditions in rice AC. The same medium was applied for induction of androgenesis and plant regeneration in a one-step method [3,29], while other protocols used different media for induction and plant regeneration in rice AC. The N 6 medium [30] has been the most frequently used medium for the induction of androgenesis over the past two decades [5][6][7][8][10][11][12][13][14][21][22][23][24][25]27,31,32].…”

Section: Introductionmentioning

confidence: 99%

Improvement of Anther Culture to integrate Doubled Haploid Technology in Temperate Rice (Oryza sativa L.) Breeding

Lantos

Jancsó

Székely

et al. 2022

Plants

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Doubled haploid (DH) plant production, such as anther culture (AC), is an effective tool used in modern rice breeding programs. The improved efficient protocols applied can shorten the process of breeding. The effect of combinations of plant growth regulators (2.5 mg/L NAA, 1 mg/L 2,4-D and 0.5 mg/L kinetin; 2 mg/L 2,4-D and 0.5 mg/L BAP) in the induction medium were compared in AC for five rice breeding materials and combinations. Induction of calli ranged from 264.6 ± 67.07 to 468.8 ± 123.2 calli/100 anthers in AC of rice genotypes. Two basal media (MS and N6) and two combinations of growth regulators (1 mg/L NAA, 1 mg/L BAP and 1 mg/L kinetin; 1.5 mg/L BAP, 0.5 mg/L NAA and 0.5 mg/L kinetin) were used as regeneration media. The in vitro green plant production was the highest with the application of the N6NDK induction medium (NAA, 2,4-D and kinetin) and the MS-based regeneration medium (1 mg/L NAA, 1 mg/BAP and 1 mg/L kinetin) in anther culture of the ‘1009’ genotype (95.2 green plantlets/100 anthers). The mean of five genotypes was 24.48 green plantlets/100 anthers for the best treatment. Flow cytometric analyses conducted identified the microspore origin of the haploid calli produced in AC, while the uniformity of spontaneous DH plants was checked in the DH1 and DH2 generations. Spontaneous chromosome doubling ranged from 38.1% to 57.9% (mean 42.1%), depending on the breeding source. The generated and selected DH lines were tested in micro- and small-plot field experiments to identify promising lines for a pedigree breeding program. The improved AC method was integrated in a Hungarian temperate rice pedigree breeding program.

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“…Since 1982, some scientists have been trying to simplify the culturing procedure and improve the efficiency of rice anther culture. They have reported on one-step methods by which callus induction and subsequent shoot regeneration occurred on the same medium (Lin et al 1984, Karim and Zapata 1990, Marassi et al 1993, Jiang and Chen 1994, Yamamoto et al 1995; similarly for wheat: Yan & Huang 1986, Liang et al 1987). However, the shoot regeneration frequencies remained too low to be applied in rice breeding and so the aim of this investigation was to improve the onestep procedure of anther culture.…”

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confidence: 99%

Phenylacetic acid stimulation of direct shoot formation in anther and somatic tissue cultures of rice (Oryza saliva L.)

Zhuo

Cheng

et al. 1996

Plant Breeding

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The effect of phenylacetic acid (PAA) on rice {Oryza sativa L.) anther culture was investigated with six genotypes, using 2,4-D as control. In the two-step culture protocol, replacing 2,4-D with PAA in the induction medium did not infiuence callus induction but significantly improved the shoot differentiation from callus, particularly in the indica cultivar Teqing. The anther-derived calli of all genotypes regenerated shoots directly on the callus induction medium containing PAA. Most of the directly-regenerated plantlets had well-developed root systems and were therefore readily transplanted into soil. The improved shoot differentiation potential and the frequency of direct regeneration depended on genotype, basal medium and PAA concentration. The one-step green shoot regeneration frequencies obtained were 1.98% with the indica cultivar '129', 1.5% with the indica x japonica hybrid 'Teqing/02428'' (F,), and 1.98% with the indica x indica hybrid 'Waiyin 2/C.B.' (Fj). The PAA-based one-step method was most effective on the anther culture of indica genotypes. Three DH populations have been constructed from hybrids (Fj) via one-step culture. PAA also enhanced the one-step plantlet formation in rice somatic tissue culture.

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Regeneration of rice double haploids using a onestep culture procedure

Cited by 6 publications

References 15 publications

A Mediterranean japonica rice (Oryza sativa) cultivar improvement through anther culture

A Mediterranean japonica rice (Oryza sativa) cultivar improvement through anther culture

Improvement of Anther Culture to integrate Doubled Haploid Technology in Temperate Rice (Oryza sativa L.) Breeding

Phenylacetic acid stimulation of direct shoot formation in anther and somatic tissue cultures of rice (Oryza saliva L.)

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