Regeneration of Rabbit Cornea Following Excimer Laser Photorefractive Keratectomy: a Study on Gap Junctions, Epithelial Junctions and Epidermal Growth Factor Receptor Expression in Correlation with Cell Proliferation

Ratkay-Traub, Imola; Hopp, B.; Bor, Zs.; Dux, L.; Becker, DL; Krenács, Tibor

doi:10.1006/exer.2001.1040

Cited by 57 publications

(36 citation statements)

References 30 publications

(28 reference statements)

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“…More recently, Morishige et al 31 confirmed Cx43 downregulation in basal cells of the rat cornea at the leading edge of the migrating epithelium, although they suggested that Cx43 levels were stable in the remaining portion of the epithelium. Remarkably, there has been little study of Cx43 between stromal keratocytes after corneal wounding, with Ratkay-Traub et al 17 only commenting that Cx43 was always detected in corneal keratocytes and endothelium and noting that "stromal reaction was usually missing in studies presented by others. "…”

Section: Discussionmentioning

confidence: 99%

“…15,29 Previous studies have indicated that connexin levels in the epithelium change after corneal wounding. Ratkay-Traub et al 17 demonstrated that Cx43 and Cx26 were upregulated in rabbit cornea after excimer corneal wounding, but Cx43 was reduced at the epithelial edge itself that was migrating to close the wound. Similarly, Suzuki et al 30 showed that actively migrating rat cornea epithelial cells lacked Cx43 gap junctions and desmosomes normally present in the basal cell layer.…”

Section: Discussionmentioning

confidence: 99%

“…15 Dong et al 16 were the first to describe the presence of Cx43 in corneal fibroblasts and documented its predominant expression in the basal cells of the rat corneal epithelium. RatkayTraub et al 17 investigated the changes in Cx43 expression after excimer laser photorefractive keratectomy in rabbits and reported Cx43 levels to be upregulated. They also showed its expression to be relocated to the upper cell layers of the epithelium 24 hours after surgery.…”

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confidence: 99%

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Improved Corneal Wound Healing through Modulation of Gap Junction Communication Using Connexin43-Specific Antisense Oligodeoxynucleotides

Grupcheva

Laux

Rupenthal

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Gap junctions play a major role in corneal wound healing. This study used reproducible models of corneal wound healing to evaluate the effect of a gap junction channel modulator, connexin43 (Cx43) antisense oligodeoxynucleotides (AsODN), on corneal healing dynamics. METHODS. A mechanical scrape wound model was used to evaluate Cx43 AsODN penetration and initial wound reepithelialization 12 hours postsurgery. Thereafter, detailed analyses of corneal edema, inflammation, and healing were performed in an excimer laser surface ablation model. In vivo confocal microscopy determined clinical parameters (edema, haze) and cellular changes (stromal hypercellularity, reepithelialization), whereas histology and immunohistochemistry were used to quantify stromal edema, inflammation, and reepithelialization. RESULTS. Cx43 AsODN penetrated through the hydrophilic stroma where the epithelium had been removed and accumulated in the basal epithelium close to the wound edge. Twelve hours after scrape wounding, Cx43 AsODN-treated eyes showed a significant reduction in wound area compared with the vehicle alone (1.59 Ϯ 0.37 and 2.29 Ϯ 0.58 mm 2 , respectively, P Ͻ 0.01). After excimer laser ablation, stromal edema and inflammation were reduced, with endothelial structures being clearly visible, and reepithelialization rates were again increased in Cx43 AsODN-treated eyes. Histologic analysis confirmed reduced edema in the central wound site and at the periphery of treated corneas (P Ͻ 0.05), whereas immunohistochemistry showed lower Cx43 levels (P Ͻ 0.05), reduced myofibroblast activation, and improved epithelial basal lamina deposition in antisense-treated wounds (P Ͻ 0.01). CONCLUSIONS. Application of Cx43 AsODN to the cornea reduces stromal edema and inflammation, promoting faster wound closure and a more uniform repair of the epithelial basal lamina after laser ablation. (Invest Ophthalmol Vis Sci.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Improved Corneal Wound Healing through Modulation of Gap Junction Communication Using Connexin43-Specific Antisense Oligodeoxynucleotides

Grupcheva

Laux

Rupenthal

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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“…Borgens et al [112][113][114][115] reported that the spinal cord responded to damage by generating large and persistent electrical signals, and in turn applied electric stimulation promoted spinal cord repair in human and other mammals. Hinkle et al [86,92,[124][125][126][127][128][129][130][131][132][133] reported that many vitro experiments showed that the electric fields were equivalent to those measured in vivo control important cell behaviors such as directional cell migration (galvanotaxis or electrotaxis), cell division orientation, and cell cycle control.…”

Section: Pathophysiological and Biological Significance Of Spontaneoumentioning

confidence: 99%

Spontaneous high-frequency action potential

Shen

Choe

2011

Sci. China Life Sci.

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Action potential, which is the foundation of physiology and electrophysiology, is most vital in physiological research. This work starts by detecting cardiac electrophysiology (tachyarrhythmias), combined with all spontaneous discharge phenomena in vivo such as wound currents and spontaneous neuropathic pain, elaborates from generation, induction, initiation, to all of the features of spontaneous high-frequency action potential--SSL action potential mechanism, i.e., connecting-end hyperpolarization initiates spontaneous depolarization and action potential in somatic membrane. This work resolves the conundrums of in vivo spontaneous discharge in tachyarrhythmias, wounds, denervation supersensitivity, neurogenic pain (hyperalgesia and allodynia), epileptic discharge and diabetic pain in pathophysiological and clinical researches that have puzzled people for a hundred years. action potential, spontaneous high-frequency action potential, tachyarrhythmias, atrial fibrillation, wound, denervation supersensitivity, neurogenic pain, hyperalgesia and allodynia, epileptic discharge, diabetic pain, regeneration and development Citation:Shen H Y, Choe W C. Spontaneous high-frequency action potential. Sci China Life Sci, 2011Sci, , 54: 311 -335, doi: 10.1007 Wound, nerve disconnection, and atrial fibrillation (AF) induced by the excessive atrial dilation, are commonly characterized by the spontaneous repetitive discharge. Their common induction is the disconnection of intercellular connection, and their common result is the initiation of spontaneous high-frequency discharge, which discloses the existence of the special action of intercellular connection.The Hodgkin-Huxley model has predicted, and the computer models in applied mathematics have well demonstrated, that potassium leakage causes persistent spontaneous electrifying state with spontaneous oscillation of high-frequency action potential. The sustained disconnection of connecting-end space induces potassium leakage in the connecting-end and consequently the sustained reduction of [K + ] i , which induces in vivo spontaneous high-frequency action potential in plasmalemma.To form an intercellular conduction, connecting-end space exerts action on ion diffusions, the alteration of the space alters ion diffusions (i.e., ionic permeability) in the connecting-end. The abolishment of connecting-end space action causes K + leakage and consequently the reduction of intracellular positive electropotential (IPE) (i.e., the increase of intracellular negative potential), which elevates resting somatic membrane potential and initiates action potential in somatic membrane. SSL action potential is named to the course from the initiation (i.e., connecting-end hyperpolarization) to the termination of somatic membrane action potential.Tachyarrhythmias that are characterized by spontaneous discharge have been most deeply studied. Their inductions contain both mechanical actions and chemical actions, and their courses contain the transformation from traditional action potential to SSL-ty...

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“…The nature of this surgical follow-up is related to the complex dynamics of the wound healing response. It involves various events such as the debris removal from the margins of the wound surface, the repair of damaged structures, the production of extracellular matrix components [7,8], as well as the replacement of cellular systems, namely the repopulation of stromal keratocytes to fill apoptotic areas [9][10][11][12], and the proliferation of epithelium to cover the ablated surface [13,14].…”

Section: Introductionmentioning

confidence: 99%

Pirenoxine prevents oxidative effects of argon fluoride excimer laser irradiation in rabbit corneas: biochemical, histological and cytofluorimetric evaluations

Cantore¹,

Siano²,

Coronnello³

et al. 2005

Journal of Photochemistry and Photobiology B: Biology

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The production of reactive oxygen species (ROS) associated with excimer laser irradiation is recognized as a possible cause of corneal haze following photorefractive keratectomy (PRK). Our work was aimed at investigating in vitro the oxidative effects induced by subablative laser fluences and at demonstrating the protective effectiveness of pirenoxine. Comparative trials of subablative fluence on rabbit eyes with or without 10 À5 M pirenoxine were carried out. Superoxide anion ðO ÀÅ 2 Þ, conjugated diene (CD), and thiobarbituric acid reagent substance (TBARS) formation were analyzed. Cellular death was evaluated by flow cytometry. Histological examinations were also performed. No appraisable differences in O ÀÅ 2 ; CD; and TBARS formation were detected soon after irradiation, whereas they all increased following incubation. Pirenoxine inhibited such increases. Cytofluorimetric and histological observations gave coherent results. The experimental data indicate that oxidative and toxic effects are ascribable to ROS avalanches triggered by laser irradiation-induced photodissociation and are inhibited by pirenoxine.

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Regeneration of Rabbit Cornea Following Excimer Laser Photorefractive Keratectomy: a Study on Gap Junctions, Epithelial Junctions and Epidermal Growth Factor Receptor Expression in Correlation with Cell Proliferation

Cited by 57 publications

References 30 publications

Improved Corneal Wound Healing through Modulation of Gap Junction Communication Using Connexin43-Specific Antisense Oligodeoxynucleotides

Improved Corneal Wound Healing through Modulation of Gap Junction Communication Using Connexin43-Specific Antisense Oligodeoxynucleotides

Spontaneous high-frequency action potential

Pirenoxine prevents oxidative effects of argon fluoride excimer laser irradiation in rabbit corneas: biochemical, histological and cytofluorimetric evaluations

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