Regeneration of Protein from Insoluble Protein-Tannin Compounds

Mejbaum-Katzenellenbogen, W; Dobryszycka, W; Bogusławska-Jaworska, J; Morawiecka, Bronisława

doi:10.1038/1841799a0

Cited by 39 publications

(14 citation statements)

References 2 publications

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“…Protein concentrations were estimated either by absorbance measurements or by the microtannin turbidity method [26] using S1, actin or rabbit A2 light chain samples of known concentrations as standards. The absorption coefficients (and molecular masses) used were: for S 3,0.8 mg .…”

Section: Preparation Of Proteinsmentioning

confidence: 99%

The widespread distribution of α‐N‐trimethylalanine as the N‐terminal amino acid of light chains from vertebrate striated muscle myosins

Henry

Trayer

Brewer

et al. 1985

European Journal of Biochemistry

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Identical tripeptides of the sequence X‐Pro‐Lys, where X is an unknown blocking group, were isolated from trypsin digests of bovine cardiac alkali light chain and the LC2 light chain of rabbit fast muscle. Chemical, electrophoretic and 1H‐NMR evidence characterized X as an unusual amino acid, α‐N‐trimethylalanine (Me3Ala), which was earlier reported as the N‐terminal amino acid of the A1 alkali light chain of rabbit fast muscle [Henry et al. (1982) FEBS Lett. 144, 11–15]. The narrow line width and chemical shift position (δ= 3.23 ppm) of the –N+ ‐(CH3) protons of Me3Ala made 1H‐NMR spectroscopy a convenient method to search for this residue in other light chains. A survey of many different light chains showed that this signal was present in all vertebrate striated muscle light chains of the A1‐type (LC1, ‘essential’ light chains) and LC2‐type (‘DTNB’‐light chains, ‘phosphorylatable’ light chains) but was absent from all invertebrate muscle and vertebrate smooth muscle light chains tested. It was also absent from the vertebrate fast‐muscle‐specific A2‐type (LC3) light chains. The spectral characteristics of these signals were consistent with their having arisen from the protons of an – N+ ‐(CH3)3 grouping. Since no ɛ‐trimethyllysine could be detected in acid hydrolysates of these proteins, it appears that Me3Ala is a general feature as the N‐terminal amino acid in these light chains. 1H‐NMR studies on bovine cardiac myosin subfragment 1 (S1) showed that the Me3Ala methyl proton signal was clearly visible and that the spectrum more closely resembled that of a rabbit S1 isoenzyme, S1(A1), than S1(A2), suggesting that the 40‐residue N‐terminal segment of the alkali light chain in cardiac S1 also possesses a high segmental mobility. Addition of actin caused the same gross changes to the cardiac S1 spectrum as noted earlier for rabbit S1(A1) [Prince et al. (1981) Eur. J. Biochem. 121, 213–219]. In particular, a marked reduction in the segmental mobility of the N‐terminal region of the alkali light chain was noted, consistent with a direct interaction of this area with actin.

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Section: Preparation Of Proteinsmentioning

confidence: 99%

The widespread distribution of α‐N‐trimethylalanine as the N‐terminal amino acid of light chains from vertebrate striated muscle myosins

Henry

Trayer

Brewer

et al. 1985

European Journal of Biochemistry

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“…Characteristically, complexation is a fast process (on the order of minutes), and in principle, is reversible. For instance, the complexation of proteins with polyphenolic tannins (to which humic acids bear many resemblances) can be reversed using caffeine (Mejbaum-Katzenellenbogen and Dobryszycka 1962; Mejbaum- Katzenellenbogen et al 1959). We experimented with a humic-collagen complex (see below), and found that a large fraction of the humic acid had apparently irreversibly bound to the collagen, and that none of the M methodologies succeeded in completely removing the humic acid (van Klinken and Hedges 1995).…”

Section: Substancesmentioning

confidence: 99%

Chemistry Strategies for Organic ¹⁴C Samples

Klinken¹,

Hedges²

1997

Radiocarbon

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ABSTRACT. Pretreatment of organic samples can be achieved by removal of contaminants, or, alternatively, by isolation of sample-specific components. We discuss the molecular aspects of these two pretreatment types, together with an assessment of their effectiveness in relation to sample type. The main division in sample type is the one between carbohydrates and proteins, leading to opposite chemical strategies for the two sample categories. Recommendations for routine 14C chemistry of organic samples also include the standardization of quality screening procedures using chemical, stable isotope and elemental data that can be collected routinely during the pretreatment of each sample.

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“…Algal cells and maternal cell walls were sedimented by centrifugation at 800 g for 10 min at 4°C. Glycoproteins were precipitated from the supernatant with tannin solution (Mejbaum- Katzenellenbogen 1959) and were liberated from the precipitate by addition of caffeine (Mejbaum-Katzenellenbogen et al 1959). The procedure was completed by freezing the precipitate with caffeine at -20°C and dissolving the liberated glycoproteins in a small quantity of distilled water.…”

Section: Preparation Of Mgps From Microalgal Culturementioning

confidence: 99%

Effects of various elicitors on the accumulation and secretion of spiroketal enol ether diacetylenes in feverfew hairy root culture

et al. 2011

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In a hairy root culture of Tanacetum parthenium treated with yeast extract (YE), silver nitrate (AgNO 3 ) and microalgal glycoproteins (MGPS), contents of four spiroketal enol ether type diacetylenes were measured. The elicitors transiently reduced contents of three constitutive spiroketal enol ethers and selectively enhanced accumulation of cis-C 13 -spiroketal enol ether epoxide ((E)-3,4-epoxy-2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4.4]nonane) in the roots. The most abundant formation of cis-C 13 -spiroketal enol ether epoxide was observed after 48-96 h of AgNO 3 treatment and 96 h of YE treatment (over 3-fold increase compared with the control). The applied elicitors caused enhanced liberation of cis-C 13 -spiroketal enol ether epoxide to the culture medium. The results show that diacetylene accumulation pattern in the elicited hairy roots is affected in a similar manner, irrespectively of the elicitor applied.

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Regeneration of Protein from Insoluble Protein-Tannin Compounds

Cited by 39 publications

References 2 publications

The widespread distribution of α‐N‐trimethylalanine as the N‐terminal amino acid of light chains from vertebrate striated muscle myosins

The widespread distribution of α‐N‐trimethylalanine as the N‐terminal amino acid of light chains from vertebrate striated muscle myosins

Chemistry Strategies for Organic ¹⁴C Samples

Effects of various elicitors on the accumulation and secretion of spiroketal enol ether diacetylenes in feverfew hairy root culture

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Regeneration of Protein from Insoluble Protein-Tannin Compounds

Cited by 39 publications

References 2 publications

The widespread distribution of α‐N‐trimethylalanine as the N‐terminal amino acid of light chains from vertebrate striated muscle myosins

The widespread distribution of α‐N‐trimethylalanine as the N‐terminal amino acid of light chains from vertebrate striated muscle myosins

Chemistry Strategies for Organic 14C Samples

Effects of various elicitors on the accumulation and secretion of spiroketal enol ether diacetylenes in feverfew hairy root culture

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Chemistry Strategies for Organic ¹⁴C Samples