Regeneration of plants from Fraxinus pennsylvanica hypocotyls and cotyledons

Du, Ningxia; Pijut, Paula M.

doi:10.1016/j.scienta.2008.05.014

Cited by 20 publications

(26 citation statements)

References 22 publications

(15 reference statements)

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“…The hypocotyls showed a greater regenerative capacity than the cotyledons. Similar findings were reported by Du and Pijut (2008) and Tonon et al (2001). The distal end of the hypocotyl segment (the section near the root meristem) produced more shoots than the proximal end (the section near the cotyledon), thereby demonstrating a strong polarity action.…”

Section: Discussionsupporting

confidence: 85%

“…However, several authors have reported direct plant regeneration from both hypocotyls (Annapurna and Rathore 2010;Nagori and Purohit 2004;Shang et al 2006;Singh et al 2002) and cotyledons (Du and Pijut 2008;Raveendar et al 2009). The data presented in this study clearly demonstrate that high-frequency adventitious shoot regeneration is possible from both hypocotyl and cotyledon segments of L. frutescens, also a woody species.…”

Section: Discussionmentioning

confidence: 99%

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Cytokinin-induced organogenesis in Lessertia (Sutherlandia) frutescens L. using hypocotyl and cotyledon explants affects yields of l-canavanine in shoots

Shaik

Singh

Nicholas

2010

Plant Cell Tiss Organ Cult

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A simple protocol for direct shoot organogenesis and plant regeneration in Lessertia frutescens using hypocotyl and cotyledon segments is reported. L-canavanine content in the derived shoots is also quantified. Media containing different concentrations and combinations of the cytokinins kinetin (K) and benzyladenine (BA) were tested for shoot induction potential. The best shoot regeneration rate (83%) was obtained from hypocotyl segments cultured in Murashige and Skoog (MS) medium supplemented with 1 mg l -1 K; these hypocotyls also produced the largest number of shoots per explant (3.5) and the longest shoots per explant (13.3 mm). The best shoot regeneration rate (46%) using cotyledons as explant material was obtained in MS medium supplemented with 1 mg l -1 K and 1 mg l -1 BA or with 5 mg l -1 K and 0.5 mg l -1 BA. The highest number of cotyledon-derived shoots (1.5) was obtained in MS medium containing 2 mg l -1 K and 0.5 mg l -1 BA, and the longest cotyledon-derived shoots (6.1 mm) were obtained in MS medium containing 1 mg l -1 K and 0.5 mg l -1 BA. Shoots derived from hypocotyls cultured on media containing 1 mg l -1 K contained the highest quantity of L-canavanine (1.42 mg g -1 ) relative to the control (0.52 mg g -1 ). Shoots derived from cotyledons cultured on media containing 2 mg l -1 K contained the highest quantity of L-canavanine (2.07 mg g -1 ) compared to the control. Scanning electron microscopy revealed that shoots regenerated directly from the wounded epidermal tissue, although callus formation was observed in most cultures. Young shoot clusters proliferated into healthy adventitious shoots that were subsequently transferred directly onto rooting medium (MS medium containing 4 mg l -1 indole-3-butyric acid), eliminating the need for an additional multiplication or elongation phase. The in vitro plants were successfully acclimatized in a growth chamber, achieving an 85% survival rate.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Cytokinin-induced organogenesis in Lessertia (Sutherlandia) frutescens L. using hypocotyl and cotyledon explants affects yields of l-canavanine in shoots

Shaik

Singh

Nicholas

2010

Plant Cell Tiss Organ Cult

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“…However, for transformation to be practical a suitable adventitious regeneration system must be established. Such protocols exist for a number of ash species including green (F. pennsylvanica), common (F. excelsior), flowering (F. ornus), and white ash (F. americana), but no protocol has been established for pumpkin ash (Arrillaga et al 1992;Du and Pijut 2008;Palla and Pijut 2011;Tabrett and Hammatt 1992). Previous studies have shown regeneration of ash possible from a variety of initial organs (Bates et al 1992;Du and Pijut 2008;Hammatt 1994;Mockeliunaite and Kuusiene 2004;Tabrett and Hammatt 1992;Tonon et al 2001).…”

Section: Introductionmentioning

confidence: 99%

“…Such protocols exist for a number of ash species including green (F. pennsylvanica), common (F. excelsior), flowering (F. ornus), and white ash (F. americana), but no protocol has been established for pumpkin ash (Arrillaga et al 1992;Du and Pijut 2008;Palla and Pijut 2011;Tabrett and Hammatt 1992). Previous studies have shown regeneration of ash possible from a variety of initial organs (Bates et al 1992;Du and Pijut 2008;Hammatt 1994;Mockeliunaite and Kuusiene 2004;Tabrett and Hammatt 1992;Tonon et al 2001). Although these studies utilized a variety of plant growth regulators and culture media, Kim et al (1997Kim et al ( , 1998 and Du and Pijut (2008) found that a Murashige and Skoog (MS;1962) medium with Gamborg et al B5 vitamins (B5;1968) in combination with thidiazuron (TDZ), 6-benzyladenine (BA), and indole-3-butyric acid (IBA) to be most effective at producing adventitious shoots in green ash.…”

Section: Introductionmentioning

confidence: 99%

Hypocotyl derived in vitro regeneration of pumpkin ash (Fraxinus profunda)

Stevens

Pijut

2011

Plant Cell Tiss Organ Cult

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Pumpkin ash (Fraxinus profunda (Bush) Bush) is at risk for extirpation by an exotic insect, the emerald ash borer (EAB). Pumpkin ash is limited to wetland areas of the Eastern United States, and has been listed as an endangered species because of EAB activity. Pumpkin ash provides many benefits to the ecosystem, and its wood is used in the manufacturing industry. In vitro regeneration provides an integral tool for the mass propagation and genetic transformation of pumpkin ash to combat EAB. Therefore, a plant regeneration protocol was developed for pumpkin ash. Aseptically extracted hypocotyls formed adventitious shoots following 4 weeks on Murashige and Skoog (MS) medium supplemented with 0-22.2 lM 6-benzyladenine (BA) and 0-6.8 lM thidiazuron (TDZ) then transferred for an additional 4 weeks on MS medium with Gamborg B5 vitamins plus 0.2 g L -1 glycine (B5G) containing 6.7 lM BA, 1 lM indole-3-butryic acid (IBA), and 0.29 lM gibberellic acid (GA 3 ). As adventitious shoots developed, these were transferred to a MSB5G medium with 13.3 lM BA, 1 lM IBA, and 0.29 lM GA 3 for shoot elongation. Elongated shoots were successfully micropropagated using MSB5 medium with 10 lM BA and 10 lM TDZ. Adventitious root formation was as high as 94% using woody plant medium supplemented with 4.9 lM IBA with shoots cultured for 10 days in the dark followed by culture under a 16-h photoperiod.Acclimatization to the greenhouse was successful and normal plant growth was observed. This protocol will provide a means for genetic transformation for EAB resistance and mass propagation for conservation.

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“…Regeneration from cotyledon explants has been reported in several taxa [21][22][23][24]36,37] but it has been recorded in only a few species belonging to Citrus genus: adventive embryos formation in Citrus reticulata Blanco (Nagpur mandarin) and C. jambhiri Lush. (Rough lemon) [5], indirect somatic embryogenesis in C. reticulata "Local Sangtra" [38] and indirect shoot regeneration in C. grandis (L.) Osbeck (pummelo) [32,39].…”

Section: Resultsmentioning

confidence: 99%