Regeneration of human auditory nerve. In vitro/in video demonstration of neural progenitor cells in adult human and guinea pig spiral ganglion

Rask‐Andersen, Helge; Boström, Marja; Gerdin, Bengt; Kinnefors, Anders; Nyberg, Gunnar; Engstrand, Thomas; Miller, Josef M.; Lindholm, Dan

doi:10.1016/j.heares.2004.12.005

Cited by 142 publications

(102 citation statements)

References 47 publications

(50 reference statements)

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“…(2,3) Consequently, loss of any differentiated neurosensory cell will potentially diminish hearing. In contrast to other vertebrates (like bony fish or chickens), there is no evidence for spontaneous regeneration of lost neurosensory cells in the mammalian cochlea in vivo.…”

Section: Introductionmentioning

confidence: 99%

The molecular basis of neurosensory cell formation in ear development: a blueprint for hair cell and sensory neuron regeneration?

2006

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SummaryThe inner ear of mammals uses neurosensory cells derived from the embryonic ear for mechanoelectric transduction of vestibular and auditory stimuli (the hair cells) and conducts this information to the brain via sensory neurons. As with most other neurons of mammals, lost hair cells and sensory neurons are not spontaneously replaced and result instead in age-dependent progressive hearing loss. We review the molecular basis of neurosensory development in the mouse ear to provide a blueprint for possible enhancement of therapeutically useful transformation of stem cells into lost neurosensory cells. We identify several readily available adult sources of stem cells that express, like the ectoderm-derived ear, genes known to be essential for ear development. Use of these stem cells combined with molecular insights into neurosensory cell specification and proliferation regulation of the ear, might allow for neurosensory regeneration of mammalian ears in the near future.

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Section: Introductionmentioning

confidence: 99%

The molecular basis of neurosensory cell formation in ear development: a blueprint for hair cell and sensory neuron regeneration?

2006

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“…The pioneering work on culturing dissociated spiral ganglion neurons was carried out more than a decade ago with embryonic rats and chicken (Lefebvre et al, 1990a;Yamaguchi and Ohmori, 1990). Various protocols have since been established for culturing spiral ganglion neurons from a range of species and ages, such as embryonic mice (Rabejac et al, 1994;Vazquez et al, 1994); neonatal mice (Kita et al, 2005;Lin et al, 1998;Mo and Davis, 1997;Whitlon et al, 2006), rats (Dazert et al, 1998;Hegarty et al, 1997;Lefebvre et al, 1990b;Malgrange et al, 1996;Marzella et al, 1997;Ripoll and Rebillard, 1997;Rome et al, 1999;Zheng et al, 1995), and gerbils (Lin, 1997); and adult rats (Lefebvre et al, 1991), guinea pigs (Anderson et al, 2006;Rask-Andersen et al, 2005), and humans (Rask-Andersen et al, 2005). Sensory epithelia have also been cultured from the cochlea of adult guinea pigs (Zhao, 2001); most experiments with spiral ganglion neurons, however, have been conducted with neonatal samples, possibly because adult neurons are in general more difficult to culture (Banker and Goslin, 1998).…”

Section: Introductionmentioning

confidence: 99%

Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice

et al. 2007

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We have developed a reliable protocol for the serum-free dissociation and culture of spiral ganglion neurons from adult mice, an important animal model for patients with post-lingual hearing loss. Pilot experiments indicated that the viability of spiral ganglion cells in vitro depended critically on the use of Hibernate medium with B27 supplement.With an optimized protocol, we obtained 2 · 10 3 neurons immediately after dissociation, or about one-fifth of those present in the intact spiral ganglion. After four days in culture, 4% of the seeded neurons survived without any exogenous growth factors other than insulin. This yield was highly reproducible in five independent experiments and enabled us to measure systematically the numbers and lengths of the regenerating neurites.Furthermore, the survival rate compared well to the few published protocols for culturing adult spiral ganglion neurons from other species. Enhanced survival and neurite outgrowth upon the addition of brain-derived neurotrophic factor and leukemia inhibitory factor demonstrated that both are potent stimulants for damaged spiral ganglion neurons in adults. This responsiveness to exogenous growth factors suggested that our culture protocol will facilitate the screening of molecular compounds as potential treatments for sensorineural hearing loss.3

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“…The belief that no tissue stem cells might exist in the inner ear was overturned by the finding that stem cells were still present in the vestibular organs of adult mice [19]. Several laboratories adopted a sphere-forming assay to isolate stem/progenitor cells from complex cell mixtures [6,19,20,21,30,33,39,37,48,51,53,54] derived from inner ear tissues. Sphere-forming cells from the utricle of adult mice are pluripotent and can give rise to a variety of cell types, including cells representative of ectodermal, mesodermal and endodermal lineages [19].…”

Section: Stem Cells In the Inner Earmentioning

confidence: 99%

Potential of Pluripotent Stem Cells for the Replacement of Inner Ears

Nishimura¹,

Nakagawa²,

Ito³

2011

Embryonic Stem Cells - Recent Advances in Pluripotent Stem Cell-Based Regenerative Medicine

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Regeneration of human auditory nerve. In vitro/in video demonstration of neural progenitor cells in adult human and guinea pig spiral ganglion

Cited by 142 publications

References 47 publications

The molecular basis of neurosensory cell formation in ear development: a blueprint for hair cell and sensory neuron regeneration?

The molecular basis of neurosensory cell formation in ear development: a blueprint for hair cell and sensory neuron regeneration?

Survival and stimulation of neurite outgrowth in a serum-free culture of spiral ganglion neurons from adult mice

Potential of Pluripotent Stem Cells for the Replacement of Inner Ears

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