Regeneration of genetically diverse plants from tissue cultures of forage grass — Panicum sps

Bajaj, Y. P. S.; Sidhu, B. S.; Dubey, Vimal Kumar

doi:10.1007/bf00033669

Cited by 19 publications

(4 citation statements)

References 7 publications

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“…Regeneration systems have now been developed for many grass species including forage (Chen et al, 1977;Lo et al, 1980;Bajaj et al, 1981;Songstad, 1983;Johnson and Worthington, 1987;Metzinger et al, 1987;Straub et al, 1989;Franklin et al, 1990;Akashi and Adachi, 1992) and turf (Lee, 1996) to ornamental (Robacker and Corley, 1992) and biofuel grasses (Denchev and Conger, 1994). Here we report the first protocol for inducing embryogenic and organogenic calluses in the forage grass Bouteloua gracilis using the shoot apex as starting material.…”

Section: Discussionmentioning

confidence: 95%

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Tissue culture and plant regeneration of blue grama grass, Bouteloua gracilis (H.B.K.) Lag. Ex Steud

Aguado-Santacruz

Cabrera-Ponce

Olalde‐Portugal³

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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As a first step towards applying biotechnology to blue grama, Bouteloua gracilis (H.B.K.) Lag. ex Steud., we have developed a regenerable tissue culture system for this grass. Shoot apices were isolated from 3-d-old seedlings and cultured in 15 different growth regulator formulations combining 2,4-dichlorophenoxyacetic acid (2,4-D), Picloram (4-amino-3,5,6-trichloropicolinic acid), N 6 -benzyladenine (BA) or adenine (6-aminopurine). The highest induction of organogenic callus was obtained with formulations containing 1 mg l 21 (4.52 mM) 2,4-D plus 0.5 mg l 21 (2.22 mM) BA, and 2 mg l 21 (8.88 mM) BA plus 1 mg l 21 (4.14 mM) Picloram with or without 40 mg l 21 (296.08 mM) adenine. Lower frequencies of induction were obtained for embryogenic as compared to organogenic callus. The most efficient treatments for induction of embryogenic callus contained 2 mg l 21 (9.05 mM) 2,4-D combined with 0.25 (1.11 mM) or 0.50 mg l 21 (2.22 mM) BA, or 1 mg l 21 (4.52 mM) 2,4-D with 0.50 mg l 21 (2.22 mM) BA. Regeneration was achieved in hormonefree Murashige anmd Skoog (MS) medium, half-strength MS medium or MS medium plus 1 mg l 21 (1.44 mM) gibberellic acid. The number of plantlets regenerated per 500 mg callus fresh weight on MS medium ranged from 9 for 2 mg l 21 (9.05 mM) 2,4-D to 62.2 for induction medium containing 2 mg l 21 (8.28 mM) Picloram, 1 mg l 21 (4.44 mM) BA and 40 mg l 21 (296.08 mM) adenine. Regenerated plants grown in soil under greenhouse conditions reached maturity and produced seeds.

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Section: Discussionmentioning

confidence: 95%

“…The shoot apex has been successfully used as explant in cereals and millets, but less frequently in forage grass tissue culture (Bajaj et al, 1981).…”

Section: Discussionmentioning

confidence: 99%

Tissue culture and plant regeneration of blue grama grass, Bouteloua gracilis (H.B.K.) Lag. Ex Steud

Aguado-Santacruz

Cabrera-Ponce

Olalde‐Portugal³

et al. 2001

In Vitro Cell.Dev.Biol.-Plant

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“…The morphology of callus has been reported and described in the important agronomic monocot crops such as maize [8][9][10][11][12][13][14], rice [15][16][17], sorghum [18], sugarcane [19], wheat [20], and various nonfood grasses [21][22][23][24][25][26]. Type I callus is the typical and most prevalent callus formed in monocot species.…”

Section: Introductionmentioning

confidence: 99%

An Improved Tissue Culture System for Embryogenic Callus Production and Plant Regeneration in Switchgrass (Panicum virgatum L.)

et al. 2009

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The increased emphasis on research of dedicated biomass and biofuel crops begs for biotechnology method improvements. For switchgrass (Panicum virgatum L.), one limitation is inefficient tissue culture and transformation systems. The objectives of this study were to investigate the utility of a new medium described here, LP9, for the production and maintenance of switchgrass callus and its regeneration, which also enables genetic transformation. LP9 medium is not based on Murashige and Skoog (MS) medium, the basal medium that all published switchgrass transformation has been performed. We demonstrate an efficient tissue culture system for switchgrass Alamo 2, which yields increased viability of callus and the ability to maintain callus for a duration of over 6 months. This longevity gives a greater useful callus lifetime than for published switchgrass MS-based media. This increased longevity enables greater potential efficiency and throughput for a transformation pipeline. Callus produced on LP9 is categorized as type II callus, which is more friable and easier to multiply, maintain and transfer than type I callus obtained from previously described tissue culture systems.

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“…Chromosome alterations, including changes in number, also were observed but not always correlated to phenotypic changes. Bajaj et al, (1981) observed changes in leaf shape in Panicum somaclones. Davies and Cohen (1992) evaluated variation in dallisgrass somaclones.…”

Section: Somaclonal Variation In Forage Grassesmentioning

confidence: 86%

Clonal Variation in Cereals and Forage Grasses

Rush

Xie

Croughan

et al. 1998

Somaclonal Variation and Induced Mutations in Crop Improvement

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Regeneration of genetically diverse plants from tissue cultures of forage grass — Panicum sps

Cited by 19 publications

References 7 publications

Tissue culture and plant regeneration of blue grama grass, Bouteloua gracilis (H.B.K.) Lag. Ex Steud

Tissue culture and plant regeneration of blue grama grass, Bouteloua gracilis (H.B.K.) Lag. Ex Steud

An Improved Tissue Culture System for Embryogenic Callus Production and Plant Regeneration in Switchgrass (Panicum virgatum L.)

Clonal Variation in Cereals and Forage Grasses

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