Regeneration of biomimetic hydroxyapatite on etched human enamel by anionic PAMAM template in vitro

Chen, Liang; Liang, Kunneng; Li, Jianshu; Wu, Dongbo; Zhou, Xuedong; Li, Jiyao

doi:10.1016/j.archoralbio.2013.03.008

Cited by 68 publications

(46 citation statements)

References 29 publications

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“…[43][44][45] To a large extent, this is due the well-known biopassivation abilities of PEO 46 and the strong interaction of poly(electrolyte)s with inorganic ions [47][48][49][50] and surfaces. 47,48,[51][52][53] The combination of highly charged remineralizition-enhancing 48,49,[54][55][56][57][58][59][60][61][62] or dissolution-limiting 63,64 polymer segments with the biopassivation of PEO is thus a viable strategy towards multifunctional polymers providing both anti-biolm and remineralization-enhancing behavior in one single system.…”

Section: 26mentioning

confidence: 99%

Poly(ethylene oxide)-based block copolymers with very high molecular weights for biomimetic calcium phosphate mineralization

et al. 2015

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The present article is among the first reports on the effects of poly(ampholyte)s and poly(betaine)s on the biomimetic formation of calcium phosphate. We have synthesized a series of di-and triblock copolymers based on a non-ionic poly(ethylene oxide) block and several charged methacrylate monomers, 2-(trimethylammonium)ethyl methacrylate chloride, 2-((3-cyanopropyl)-dimethylammonium)ethyl methacrylate chloride, 3-sulfopropyl methacrylate potassium salt, and [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl) ammonium hydroxide. The resulting copolymers are either positively charged, ampholytic, or betaine block copolymers. All the polymers have very high molecular weights of over 10 6 g mol À1 . All polymers are water-soluble and show a strong effect on the precipitation and dissolution of calcium phosphate.The strongest effects are observed with triblock copolymers based on a large poly(ethylene oxide) middle block (nominal M n ¼ 100 000 g mol À1 ). Surprisingly, the data show that there is a need for positive charges in the polymers to exert tight control over mineralization and dissolution, but that the exact position of the charge in the polymer is of minor importance for both calcium phosphate precipitation and dissolution.

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Section: 26mentioning

confidence: 99%

Poly(ethylene oxide)-based block copolymers with very high molecular weights for biomimetic calcium phosphate mineralization

et al. 2015

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“…Phosphoric acid at concentration from 30% to 37% was used in 17 studies. In addition, a demineralising solution [22,23,24,25,26], citric acid [27], nitric acid [28,29,30], acidified gelatine gel [31], and a bi-layer demineralisation protocol of methylcellulose gel buffered with a lactic acid layer [32] were also used. In fact, the different demineralization agents are used to create two different models of enamel defects.…”

Section: Resultsmentioning

confidence: 99%

“…In one study, an enamel matrix derivative was mixed with calcium chloride agarose hydrogel to guide the continuous growth of crystals, prevent the crystal fusion of premature crystals, and control the nucleation and growth of the enamel prism-like crystals [35]. A polyamidoamine (PAMAM) dendrimer solution was applied to the etched enamel surface to provide nucleation sites and a mineralisation template for nanorod-like HAP formation with high uniformity [30,36,37]. Peptide was the most commonly used protein analogue.…”

Section: Resultsmentioning

confidence: 99%

“…PAMAM dendrimers have also been used as “artificial proteins” to evaluate their binding capacity and the surface charge with enamel crystals [12,47]. PAMAM-COOH acted as the organic template on the demineralized enamel surface to induce the formation of HAP crystals with the same structure, orientation and mineral phase of the natural enamel [30]. Due to the specific adsorption on HAP, alendronate was conjugated to PAMAM-COOH and induced in situ remineralisation of HAP on acid-etched enamel [36].…”

Section: Discussionmentioning

confidence: 99%

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Methods for Biomimetic Mineralisation of Human Enamel: A Systematic Review

Cao

Mei²,

et al. 2015

Materials

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Biomimetic mineralisation is an alternative restorative methodology that imitates the natural process of mineralisation. We aimed to systematically review the laboratory methods on the biomimetic mineralisation of demineralised enamel. A search in the PubMed, ScienceDirect, and ISI Web of Science databases was performed. Clinical trials, reviews, non-English articles, animal teeth, non-tooth substrates, and irrelevant studies were excluded. After screening the titles and abstracts of initially searched articles, 20 papers remained for full-text analysis. Eight articles were identified from the references of the remaining papers. A total of 28 studies were included in this systematic review. We found that protein or protein analogues were used to mimic the function of natural protein in 23 studies. Bioactive components inspired by mussel, an agarose hydrogel model, a glycerine-enriched gelatine technique, and ethylenediaminetetraacetic acid, were also used for biomimetic mineralisation of enamel. These laboratory studies reported success in the biomimetic mineralisation of enamel. Potential further research on the biomimetic mineralisation of enamel was discussed.

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“…Hannig and Hannig [2012] have presented an overview of the status of nanoparticles in caries therapy. Although promising in vitro findings have been reported [Huang et al, 2009[Huang et al, , 2010[Huang et al, , 2011Tschoppe et al, 2011;Chen et al, 2013;Besinis et al, 2014], clinical data are still scarce. Najibfard et al [2011] measured remineralization of presoftened enamel blocks in an in situ study.…”

Section: Casein Phosphopeptide-stabilized Amorphous Calcium Phosphatementioning

confidence: 99%

Noninvasive Dentistry: A Dream or Reality?

Clarkson

Exterkate

2015

Caries Res

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Various caries prevention and repair strategies are reviewed in this article ranging from the use of fluoride to nanohydroxyapatite particles. Several of the strategies which combine fluoride and calcium and phosphate treatments have both in vitro and in vivo data showing them to be efficacious if the surface integrity of the lesion is not breached. Once this has occurred, the rationale for cutting off the nutrient supplies to the pathogenic bacteria without the removal of the infected dentine, a noninvasive restorative technique, is discussed using existing clinical studies as examples. Finally two novel noninvasive restorative techniques using fluorohydroxyapatite crystals are described. The need for clinical data in support of emerging caries-preventive and restorative strategies is emphasized.

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Regeneration of biomimetic hydroxyapatite on etched human enamel by anionic PAMAM template in vitro

Cited by 68 publications

References 29 publications

Poly(ethylene oxide)-based block copolymers with very high molecular weights for biomimetic calcium phosphate mineralization

Poly(ethylene oxide)-based block copolymers with very high molecular weights for biomimetic calcium phosphate mineralization

Methods for Biomimetic Mineralisation of Human Enamel: A Systematic Review

Noninvasive Dentistry: A Dream or Reality?

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