Regeneration of ascending spinal axons in goldfish

Hanna, G.F.B; Nawar, N.N.Y.; Sharma, S. C.

doi:10.1016/s0006-8993(98)00105-x

Cited by 18 publications

(11 citation statements)

References 57 publications

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“…In contrast to our findings in zebrafish, Hanna et al (1998) described the reestablishment of terminal fields of ascending fibers in the brain of goldfish. However, they did not show labeled somata in the spinal cord caudal to the lesion site, and they used a different tracing paradigm that is not comparable to ours.…”

Section: Regenerating Spinal Axons Change Their Pathway To the Gray Mcontrasting

confidence: 99%

“…Whereas terminal fields of ascending fibers have been described in distinct locations in the brain of teleosts after spinal tracer application (Murakami and Ito, 1985;Oka et al, 1986a;Becker et al, 1997;Hanna et al, 1998;Prasada Rao and Sharma, 1999;Finger, 2000), the origin of these fibers in the spinal cord has not been well investigated (Meek and Nieuwenhuys, 1998). After labeling from the brainstem/spinal cord transition zone, we observed a number of cells, mostly in the medial and dorsal spinal cord, that were filled retrogradely in unlesioned animals (data not shown).…”

Section: Regenerating Spinal Axons Change Their Pathway To the Gray Mmentioning

confidence: 72%

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Regenerating descending axons preferentially reroute to the gray matter in the presence of a general macrophage/microglial reaction caudal to a spinal transection in adult zebrafish

Becker

2001

J of Comparative Neurology

105

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We analyzed pathway choices of regenerating, mostly supraspinal, descending axons in the spinal cord of adult zebrafish and the cellular changes in the spinal cord caudal to a lesion site after complete spinal transection. Anterograde tracing (by application of the tracer rostral to the spinal lesion site) showed that significantly more descending axons (74%) regenerated in the spinal gray matter of the caudal spinal cord than would be expected from random growth. Retrograde tracing (by application of the tracer caudal to the spinal lesion site) showed that, rostral to the lesion, most of these axons (80%) extended into the major white matter tracts. Thus, ventral descending tracts often were devoid of labeled axons caudal to a spinal lesion but contained many axons rostral to the lesion in the same animals, indicating a pathway switch of descending axons from the white matter to the gray matter. Ascending axons of spinal neurons were not observed regrowing to the rostral tracer application site; therefore, they most likely did not contribute to the axonal populations analyzed. A macrophage/microglia response within 2 days of spinal cord transection, along with phagocytosis of myelin, was observed caudal to the transection by immunohistochemistry and electron microscopy. Nevertheless, caudal to the lesion, descending tracts in the white matter were filled with myelin debris during the time of axonal regrowth, at least up to 6 weeks postlesion. We suggest that the spontaneous regeneration of axons of supraspinal origin after spinal cord transection in adult zebrafish may be due in part to the axons' ability to negotiate novel pathways in the spinal cord gray matter.

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Section: Regenerating Spinal Axons Change Their Pathway To the Gray Mcontrasting

confidence: 99%

Section: Regenerating Spinal Axons Change Their Pathway To the Gray Mmentioning

confidence: 72%

Regenerating descending axons preferentially reroute to the gray matter in the presence of a general macrophage/microglial reaction caudal to a spinal transection in adult zebrafish

Becker

2001

J of Comparative Neurology

105

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“…In goldfish, successful regeneration of ascending axons has been reported (Hanna et al, 1998). Here, we directly demonstrate axonogenesis from new and mature neurons.…”

Section: Discussionmentioning

confidence: 67%

Fgf-Dependent Glial Cell Bridges Facilitate Spinal Cord Regeneration in Zebrafish

et al. 2012

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Adult zebrafish show a remarkable capacity to regenerate their spinal column after injury, an ability that stands in stark contrast to the limited repair that occurs within the mammalian CNS post-injury. The reasons for this interspecies difference in regenerative capacity remain unclear. Here we demonstrate a novel role for Fgf signaling during glial cell morphogenesis in promoting axonal regeneration after spinal cord injury. Zebrafish glia are induced by Fgf signaling, to form an elongated bipolar morphology that forms a bridge between the two sides of the resected spinal cord, over which regenerating axons actively migrate. Loss of Fgf function inhibits formation of this "glial bridge" and prevents axon regeneration. Despite the poor potential for mammalian axonal regeneration, primate astrocytes activated by Fgf signaling adopt a similar morphology to that induced in zebrafish glia. This suggests that differential Fgf regulation, rather than intrinsic cell differences, underlie the distinct responses of mammalian and zebrafish glia to injury.

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“…Recently, direct connections from the spinal cord to the telencephalon have been described on the basis of anterograde tracing experiments in two cypriniform teleosts: zebrafish (Becker et al, 1997) and goldfish (Hanna et al, 1998). However, in neither case were these extensive rostral projections confirmed by retrograde methods.…”

Section: Ascending Spinal Connectionsmentioning

confidence: 99%

“…These studies generally reveal ascending systems terminating in the medullary ventrolateral reticular formation, vestibular nuclei, and cerebellum. In addition, recent studies on ostariophysean fishes (goldfish and zebrafish) report direct spinal projections to the diencephalon and telencephalon, but these have not been confirmed by retrograde methods (Becker et al, 1997;Hanna et al, 1998;Prasada Rao and Sharma, 1999). A multisynaptic thalamopetal spinal lemniscus arising from rostral cervical levels has been reported in another scorpaeniform teleost .…”

mentioning

confidence: 96%

Ascending spinal systems in the fish,Prionotus carolinus

Finger

2000

J. Comp. Neurol.

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Regeneration of ascending spinal axons in goldfish

Cited by 18 publications

References 57 publications

Regenerating descending axons preferentially reroute to the gray matter in the presence of a general macrophage/microglial reaction caudal to a spinal transection in adult zebrafish

Regenerating descending axons preferentially reroute to the gray matter in the presence of a general macrophage/microglial reaction caudal to a spinal transection in adult zebrafish

Fgf-Dependent Glial Cell Bridges Facilitate Spinal Cord Regeneration in Zebrafish

Ascending spinal systems in the fish,Prionotus carolinus

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