Regeneration of <i>in Vitro</i> Shoot and Root Structure through Hormone Manipulation of Coconut (MATAG F2) Zygotic Embryos

Abstract

Zygotic embryos tissues have found to be more responsive explants for clonal propagation of coconut. In the present study, the feasibility of using zygotic embryos explants for clonal propagation of a local coconut variety MATAG F2 was assessed. Callus was induced by incorporating of cytokinin and auxin into the medium. The sliced embryos explants were immersed in 1 M maltose for 60 mins, then with 0.05 M maltose for 1 min and followed by 0.01 M maltose for 5 mins was the best for prevention of phenolic compounds excretion. The callus formation depended on the concentration of 2,4-D in the media and the best effect was observed with the high level (2,4-D and BAP) tested. Attempts at inducing multiple shoot from the zygotic embryos callus were unsuccessful. No multiple shoots was present; most of the callus became root structure.