Regeneration in axolotls: a model to aim for!

Roy, Stéphane; Gatien, Samuel

doi:10.1016/j.exger.2008.09.003

Cited by 58 publications

(46 citation statements)

References 63 publications

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“…Likewise, age-related cancer, and tumour formation in general, is very rare in the Decapoda (Vogt 2008a), although some species like lobsters can grow nearly as old as humans. Such a scarcity of age-related diseases and tumours was also found in vertebrates with indeterminate growth and high regeneration capacity such as the axolotl (Roy and Gatien 2008) and is apparently related to the preservation of stem cell integrity throughout life. Crayfish have stem cell systems that are active until old age as discussed above but also have effective protection and detoxification mechanisms involving anti-oxidative enzymes, free radical scavengers, metallothioneins and cytochrome P450 (James and Boyle 1998;Leignel et al 2008;Beytut et al 2009).…”

Section: Age-related Diseases and Stem Cell Diseasesmentioning

confidence: 97%

Suitability of the clonal marbled crayfish for biogerontological research: a review and perspective, with remarks on some further crustaceans

Vogt

2010

Biogerontology

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This article examines the suitability of the parthenogenetic marbled crayfish for research on ageing and longevity. The marbled crayfish is an emerging laboratory model for development, epigenetics and toxicology that produces up to 400 genetically identical siblings per batch. It is easily cultured, has an adult size of 4-9 cm, a generation time of 6-7 months and a life span of 2-3 years. Experimental data and biological peculiarities like isogenicity, direct development, indeterminate growth, high regeneration capacity and negligible senescence suggest that the marbled crayfish is particularly suitable to investigate the dependency of ageing and longevity from non-genetic factors such as stochastic developmental variation, allocation of metabolic resources, damage and repair, caloric restriction and social stress. It is also well applicable to examine alterations of the epigenetic code with increasing age and to identify mechanisms that keep stem cells active until old age. As a representative of the sparsely investigated crustaceans and of animals with indeterminate growth and extended brood care the marbled crayfish may even contribute to evolutionary theories of ageing and longevity. Some relatives are recommended as substitutes for investigation of topics, for which the marbled crayfish is less suitable like genetics of ageing and achievement of life spans of decades under conditions of low food and low temperature. Research on ageing in the marbled crayfish and its relatives is of practical relevance for crustacean fisheries and aquaculture and may offer starting points for the development of novel anti-ageing interventions in humans.

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Section: Age-related Diseases and Stem Cell Diseasesmentioning

confidence: 97%

Suitability of the clonal marbled crayfish for biogerontological research: a review and perspective, with remarks on some further crustaceans

Vogt

2010

Biogerontology

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“…Some vertebrates, however, possess remarkable capacities to regenerate complex body parts following injury (reviewed [2,3]). For example, certain newts and salamanders completely regenerate limbs, tails, jaw, and eye lens following removal (reviewed in [4]). Frogs, particularly during their larval tadpoles stages, have remarkable capacities to regenerate tissues following traumatic injury (reviewed in [5,6]).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of gene expression during Xenopus tropicalis tadpole tail regeneration

et al. 2011

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BackgroundThe molecular mechanisms governing vertebrate appendage regeneration remain poorly understood. Uncovering these mechanisms may lead to novel therapies aimed at alleviating human disfigurement and visible loss of function following injury. Here, we explore tadpole tail regeneration in Xenopus tropicalis, a diploid frog with a sequenced genome.ResultsWe found that, like the traditionally used Xenopus laevis, the Xenopus tropicalis tadpole has the capacity to regenerate its tail following amputation, including its spinal cord, muscle, and major blood vessels. We examined gene expression using the Xenopus tropicalis Affymetrix genome array during three phases of regeneration, uncovering more than 1,000 genes that are significantly modulated during tail regeneration. Target validation, using RT-qPCR followed by gene ontology (GO) analysis, revealed a dynamic regulation of genes involved in the inflammatory response, intracellular metabolism, and energy regulation. Meta-analyses of the array data and validation by RT-qPCR and in situ hybridization uncovered a subset of genes upregulated during the early and intermediate phases of regeneration that are involved in the generation of NADP/H, suggesting that these pathways may be important for proper tail regeneration.ConclusionsThe Xenopus tropicalis tadpole is a powerful model to elucidate the genetic mechanisms of vertebrate appendage regeneration. We have produced a novel and substantial microarray data set examining gene expression during vertebrate appendage regeneration.

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“…Axolotls have the ability to continuously regenerate their tails after amputation with intact connective and supportive tissues, muscles, nerves, skin, and blood vessels. Therefore, it represents a reliable model for studying the basic mechanisms of regeneration in vertebrates (Roy and Gatien, 2008). The axolotl has long been used for studies of development and regeneration and is still a favored model animal for these disciplines today (Epperlein and Lofberg, 1990;Voss et al, 2009;Kurth et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Ion imaging during axolotl tail regeneration in vivo

Özkucur

Epperlein

Funk

2010

Developmental Dynamics

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Several studies have reported that endogenous ion currents are involved in a wide range of biological processes from single cell and tissue behavior to regeneration. Various methods are used to assess intracellular and local ion dynamics in biological systems, e.g., patch clamping and vibrating probes. Here, we introduce an approach to detect ion kinetics in vivo using a noninvasive method that can electrophysiologically characterize an entire experimental tissue region or organism. Ion-specific vital dyes have been successfully used for live imaging of intracellular ion dynamics in vitro. Here, we demonstrate that cellular pH, cell membrane potential, calcium, sodium and potassium can be monitored in vivo during tail regeneration in the axolotl (Ambystoma mexicanum) using ion-specific vital dyes. Thus, we suggest that ion-specific vital dyes can be a powerful tool to obtain electrophysiological data during crucial biological events in vivo.

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Regeneration in axolotls: a model to aim for!

Cited by 58 publications

References 63 publications

Suitability of the clonal marbled crayfish for biogerontological research: a review and perspective, with remarks on some further crustaceans

Suitability of the clonal marbled crayfish for biogerontological research: a review and perspective, with remarks on some further crustaceans

Genome-wide analysis of gene expression during Xenopus tropicalis tadpole tail regeneration

Ion imaging during axolotl tail regeneration in vivo

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