Regeneration from long-term embryogenic callus of the Rosa hybrida cultivar kardinal

Kamo, Kathryn; Jones, B.; Bolar, Jyothi Prakash; Smith, F. D.

doi:10.1079/ivp2004599

Cited by 10 publications

(6 citation statements)

References 22 publications

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“…The induction of organic or embryogenic callus was usually promoted by an auxin, especially 2,4-D (Carman 1990, Bennici and Bruschi 1999, Kamo et al 2005. Similar results of 2,4-D in callus induction were observed.…”

Section: Resultsmentioning

confidence: 66%

In vitro Plantlet Regeneration from Callus Culture of Trachyspermum copticum

Teymourian

Ebrahimi

Tohidfar

et al. 2017

Plant Tissue Cult. & Biotech.

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The effect of explant sources and plant growth regulators on callus induction and plantlet regeneration of Trachyspermum copticum were explored. Different explants including hypocotyl, cotyledonary node and leaf were cultured on MS supplemented with different combinations and concentrations of plant growth regulators including 2,4-D (0.2-3 0.5 mg/l), NAA (2 mg/l), BAP (1-3 mg/l), Kn (0.5 mg/l) and IAA (0.8 mg/l). The best response for callus induction (100%) as well as quality was observed from cotyldonary node segments cultured on MS supplemented with 2, 4-D at 1 mg/l in combination with Kn at 0.5 mg/l. Calli derived from various explants were subcultured on shoot induction media with different compositions and concentrations of medium. MS without any plant growth regulator promoted the highest frequency of shoot regeneration (100%) and also mean number of developed shoots per explants (3.8) showed the same result. Regenerated shoots were then rooted on three-fourth strength MS with 75% efficiency after 30 days.

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Section: Resultsmentioning

confidence: 66%

In vitro Plantlet Regeneration from Callus Culture of Trachyspermum copticum

Teymourian

Ebrahimi

Tohidfar

et al. 2017

Plant Tissue Cult. & Biotech.

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“…In addition, appropriate hormone types and concentrations are the basis for embryogenic calli induction. Kamo et al (2005) found that the embryogenic calli of red rose cultivar Kardinal with SH medium supplemented with 13.6 μM 2,4-D was better than that with MS medium supplemented with 18.1 μM dicamba and 0.46 μM kinetin because the number of mature embryos increased by three times. Kim et al (2003) found that immature zygotic embryos of Rosa hybrida L. did not form somatic embryos or embryogenic calli in ½ MS medium supplemented with different concentrations of 2,4-D. Embryogenic calli did form on a medium containing 1.36 μM 2,4-D and 4.44 μM benzylaminopurine at a frequency of 25%.…”

Section: Discussionmentioning

confidence: 96%

Somatic Embryogenesis In Rosa Chinensis CV. ‘Parson’s Pink China’

Cai

Tang

Chen

et al. 2022

Preprint

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This study investigated the effects of explant type, plant growth regulator concentration, callis status, medium conversion time, and medium tilt on the growth of rose somatic embryos. The results showed that Rosa chinensis cv. ‘Parson’s Pink China’ leaves could induce normal embryogenic calli but petioles could not. When the 2,4-dichlorophenoxyacetic acid concentration was 3.0 mg/L, the callis induction rate was the highest in the embryo proliferation medium (EP medium) supplemented with 0.5mg/L kinetin, and white and reddish-brown translucent calli were the main type of embryogenic calli induced. As the culture time in EP medium was extended, the relative induction rate for secondary embryos and multicotyledon secondary embryos gradually increased when transferred to embryo maturation medium (EM medium), but the induction rate for somatic embryos decreased. Placing the EM medium at an angle of 45° made the somatic embryos germinate faster and the germination rate was also higher. The germination buds produced by the somatic embryos with two cotyledons showed the fastest germination and greatest survival rates. The results of this experiment will help improve somatic embryo regeneration rates and explore new ways of regeneration, and lays the foundation for further optimization of the somatic embryo genetic transformation system.

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“…Friable organogenic callus developed directly from nodal cuttings were periodically subcultured on MS medium supplemented with 2,4-D (2 lM). The induction of organic or embryogenic callus was usually promoted by an auxin, especially 2,4-D (Carman 1990;Bennici and Bruschi 1999;Kamo et al 2005). In the present study, a combination of high cytokinin (BA) and low auxin (2,4-D) induced organic calli from cultured nodal segments.…”

Section: Discussionmentioning

confidence: 99%

Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight & Arnott, a rare medicinal plant

Thomas

2008

Plant Biotechnol Rep

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An efficient micropropagation protocol based on multiple shoot induction and callus regeneration has been standardized in Sarcostemma brevistigma, a rare medicinal plant. The nodal cuttings were cultured on MS medium supplemented with BA (0.5-8 lM) or Kn (0.5-8 lM) alone or in combination with NAA (0.5-1.5 lM). Maximum multiple shoot induction was observed on MS medium supplemented with 4 lM BA. On this medium, 100% cultures responded with an average number of 11.3 shoots per explant. However, the average shoot length was limited to only 0.9 cm on this medium. The addition of 1 lM NAA along with 4 lM BA gave rise to an average number of 10.9 shoots with an average shoot length of 1.8 cm. Luxuriantly growing callus was obtained on MS medium supplemented with BA (5 lM) and 2,4-D (2 lM). The callus was subcultured on MS medium supplemented with BA (2-15 lM) or Kn (2-15 lM) alone or in combination with NAA (0.5-2 lM) for shoot organogenesis. Optimum callus regeneration was obtained on MS medium supplemented with 10 lM BA and 1 lM NAA. On this medium, 100% cultures responded with an average number of 13.4 shoots per culture. The shoots obtained via multiple shoot induction and organogenesis were rooted on halfstrength MS medium supplemented with NAA (1-7 lM) or IBA (1-7 lM). IBA was better than NAA in terms of both the percentage of cultures that responded and the average number of roots per explant. The rooted shoots were successfully transplanted to soil with 86% success.This standardized protocol will help to conserve this rare medicinal plant.

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Regeneration from long-term embryogenic callus of the Rosa hybrida cultivar kardinal

Cited by 10 publications

References 22 publications

In vitro Plantlet Regeneration from Callus Culture of Trachyspermum copticum

In vitro Plantlet Regeneration from Callus Culture of Trachyspermum copticum

Somatic Embryogenesis In Rosa Chinensis CV. ‘Parson’s Pink China’

Multiple shoot induction and callus regeneration in Sarcostemma brevistigma Wight & Arnott, a rare medicinal plant

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