Regeneration from callus of Undaria pinnatifida (Harvey) Suringar (Laminariales, Phaeophyta)

Abstract

Calli were formed on the explants of midrib, meristem and immature stipe parts from freshly collected Undaria pinnatifida sporophytes. Each part was sterilized by Betadine and ethanol, and was cut into explants. The explants were incubated on an agar medium at 10 hours light and 14 hours dark photoperiod under a photon flux density of 80 #mol m-2 s -. Callus was formed best on the explants of meristem parts at a temperature of 13 C on PESI medium. Calli were cut off from the explants and were transferred into a sterile liquid PESI medium in flasks. Callus was dark brown in colour and was composed of well-pigmented cells. The cells were loosely bound and were separated by low power sonication, and were easy to attach to vinylon strings. From the calli formed on the explants of meristem parts, entire fronds were regenerated, but from the calli formed on the explants of midrib parts, only thin layered laminae were regenerated. The calli formed on the explants of immature stipe parts did not exhibit any regeneration at all.

Morphogenetic responses from protoplasts and tissue culture of Laminaria digitata (Linnaeus) J. V. Lamouroux (Laminariales, Phaeophyta): callus and thalloid-like structures regeneration

“…It is now well known that, in land plants, embryogenic and meristematic tissues have a higher regeneration potential for tissue and protoplast cultures compared to mature explants because of their totipotency (Davey et al 2005). Likewise, in seaweeds with highly differentiated thalli, meristematic areas and juvenile thalli were reported to be effective for micropropagation from protoplast and tissue culture (Rhodophyta, Titlyanov et al 2006a, b;Phaeophyta, Mussio and Rusig 2006;Benet et al 1997;Kawashima and Tokuda 1993;Kloareg and Quatrano 1987).…”

“…In Laminariales species, many reports have described tissue culture, particularly in the genera Undaria (Kimura and Notoya 1997;Kawashima and Tokuda 1993;Notoya and Aruga 1992a;Yan 1984;Fang et al 1983;Zongxi et al 1983), Ecklonia (Kimura and Notoya 1996;Notoya et al 1994;Kawashima et al 1992;Aruga 1989, 1992b;Kawashima and Tokuda 1990;Lawlor et al 1987Lawlor et al , 1989Notoya 1988), Macrocystis (Ar Gall et al 1996;Polne-Fuller and Gibor 1987), Eckloniopsis (Notoya 1997;Notoya et al 1994), Egregia (Polne-Fuller andGibor 1987), Eisenia (Notoya and Aruga 1990) and Agarum (Notoya et al 1994). Tissue cultures of the genus Laminaria have been reported in seven species: Laminaria angustata (Saga and Sakai 1983;Saga et al 1978), L. digitata (Asensi et al 2001;Ar Gall et al 1996;Folefack and Cosson 1995;Liu and Kloareg 1992;Butler 1989;Fries 1980), L. hyperborea (Fries 1980), L. japonica (Wang et al 1998;Yan 1984;Fang et al 1983;Zongxi et al 1983;Saga and Sakai 1977), L. saccharina (Ar Gall et al 1996;Butler 1989;Lee 1985), L. setchellii (Qi et al 1995) and…”

Effects of ambient calcium concentration on morphological form of callus-like cells in Saccharina japonica (Phaeophyceae) sporophyte

“…Unpigmented filamentous callus-like cells were observed in explants from the thalli or stipes of Ecklonia cava (Notoya and Aruga 1989;Kawashima and Tokuda 1990), Undaria pinnatifida (Kawashima and Tokuda 1993), Laminaria digitata, Laminaria hyperborea (Fries 1980), and Saccharina lattissima (= Laminaria saccharina; Lee 1985). On the other hand, the propagation of pigmented round callus-like cells was reported in L. digitata (Asensi et al 2001).…”

Offshore and Multi-Use Aquaculture with Extractive Species: Seaweeds and Bivalves

“…Selective breeding and intensive selection of kelp strains in Asia, however, have reduced the genetic diversity and narrowed the germplasm base of the varieties in cultivation. This has led to decreased adaptability of these varieties to environmental changes and jeopardizes the industry expansion in Asia (Kawashima and Tokuda 1993;Li et al 2009;Robinson et al 2013). In North America and Europe, strain development will be a challenge.…”