Regeneration after experimental removal of skin in man

Bishop, George H.

doi:10.1002/aja.1000760202

Cited by 84 publications

(46 citation statements)

References 6 publications

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“…31 In deeper wounds, re-epithelialization typically occurs from the periphery and is mediated by the recruitment of cells from wound-adjacent IFE, HFs and glands. 32 Consistent with a role for HFs in regeneration, the hair cycle can affect the rate of wound closure in mice, 33 while in Edaradd mutant mice lacking primary HFs, wound healing in tail skin is delayed. 34 In spite of these and other findings, however, it has recently been proposed that IFE-derived cells, but not HF-derived cells, are the major long-term contributors to wound healing.…”

Section: Discussionmentioning

confidence: 40%

Hair follicle and interfollicular epidermal stem cells make varying contributions to wound regeneration

2015

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Upon wounding, multiple stem cell populations in the hair follicle (HF) and interfollicular epidermis (IFE) converge at the site of injury. Although these cells can contribute permanently to the regenerating epithelium, it remains unclear whether these contributions vary among cells originating from diverse compartments in the skin. By comparing the fates of several keratinocyte lineages, we observed here an initial decrease in both HF-and IFE-derived cells within the transient acanthotic layers of the regenerating epithelium. At the same time, the relative abundance of early-arriving IFE-derived cells specifically in the wound basal layer declined as later-arriving HF-derived cells entered the site of injury. Although laggard bulge-derived cells were typically constrained at the regenerative periphery, these cells persisted in the wound basal layer. Finally, suppressing Notch enabled IFE-derived cells to out-compete HF-derived cells. Taken together, these findings indicate that IFE-, HF-and bulge-derived cells make distinct contributions to regeneration over time. Furthermore, we speculate that extrinsic, non-genetic factors such as spatial constraint, distance from the wound, and basal versus suprabasal position may largely determine whether a cell ultimately persists.

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Section: Discussionmentioning

confidence: 40%

Hair follicle and interfollicular epidermal stem cells make varying contributions to wound regeneration

2015

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“…In order to further elucidate the molecular basis of these effects, and to verify these data in early passage human keratinocytes, we studied the effects of various biotin concentrations on proliferation, differentiation and keratin expression of outer root sheath (ORS) cells from human hair follicles. It is well known that the cells from the ORS tissue can differentiate towards interfollicular epidermal keratinocytes, as during wound healing, where they migrate out of the ORS to cover the denuded area [2,11]. Moreover, experiments in the rate have demonstrated that ORS cells have the potential to regenerate the hair matrix (i.e.…”

Section: Introductionsupporting

confidence: 39%

Proliferation and differentiation of cultured human follicular keratinocytes are not influenced by biotin

et al. 1996

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In humans and in animals, biotin deficiency causes pathological changes in the skin and its appendages. High doses of biotin may also have beneficial effects on skin, hair and fingernails in humans and animals with normal biotin status. Therefore, we investigated the effects of low and high concentrations of biotin on proliferation and differentiation of cultured outer root sheath cells from human hair follicles as an in vitro model for skin. The activities of biotin-dependent carboxylases were measured to evaluate the biotin status of the cells. In monolayer cultures of outer root sheath cells, proliferation and expression of the differentiation-specific keratins K1 and K10 were not influenced by extremely low concentrations of biotin (<2 x 10(-10) mol/l) or by pharmacological doses of biotin (10(-5) mol/l). Biotin deficiency of the cells was confirmed under the former condition by demonstrating decreased activities of the mitochondrial carboxylases. In organotypic cocultures of outer root sheath cells and dermal fibroblasts, in which stratified epithelia resembling epidermis were developed, the biotin concentration had no effect on the expression of all tested epidermal differentiation markers, including the suprabasal keratins K1 and K10, the hyperproliferation-associated keratin K16, involucrin and filaggrin.

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“…This is illustrated by the observation of faster wound healing in hair-bearing regions of humans 72, 73 and the delayed acute wound healing in the hair-less tail epidermis of mutant mice with an impaired Eda receptor 74 .…”

Section: Wound Induced Hair Follicle Neogenesis In the Adultmentioning

confidence: 99%

Therapeutic strategy for hair regeneration: hair cycle activation, niche environment modulation, wound-induced follicle neogenesis, and stem cell engineering

Chueh

Lin

Chen

et al. 2013

Expert Opinion on Biological Therapy

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Introduction There are major new advancements in the fields of stem cell biology, developmental biology, regenerative hair cycling, and tissue engineering. The time is ripe to integrate, translate and apply these findings to tissue engineering and regenerative medicine. Readers will learn about new progress in cellular and molecular aspects of hair follicle development, regeneration and potential therapeutic opportunities these advances may offer. Areas covered Here we use hair follicle formation to illustrate this progress and to identify targets for potential strategies in therapeutics. Hair regeneration is discussed in four different categories. (1) Intra-follicle regeneration (or renewal) is the basic production of hair fibers from hair stem cells and dermal papillae in existing follicles. (2) Chimeric follicles via epithelial-mesenchymal recombination to identify stem cells and signaling centers. (3) Extra-follicular factors including local dermal and systemic factors can modulate the regenerative behavior of hair follicles, and may be relatively easy therapeutic targets. (4) Follicular neogenesis means the de novo formation of new follicles. In addition, scientists are working to engineer hair follicles, which require hair forming competent epidermal cells and hair inducing dermal cells. Expert opinion Ideally self-organizing processes similar to those occurring during embryonic development should be elicited with some help from biomaterials.

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Regeneration after experimental removal of skin in man

Abstract: EIGHT FIGURESThis work centers about the regeneration of connective tissue of the clerma, after removal of or injury to the superficial layers of the skin. Interest in this problem arose from a previous study (Bishop, '44) in which patches of skin were 153 TlTr 1NIERICAN J O U R N

Cited by 84 publications

References 6 publications

Hair follicle and interfollicular epidermal stem cells make varying contributions to wound regeneration

Hair follicle and interfollicular epidermal stem cells make varying contributions to wound regeneration

Proliferation and differentiation of cultured human follicular keratinocytes are not influenced by biotin

Therapeutic strategy for hair regeneration: hair cycle activation, niche environment modulation, wound-induced follicle neogenesis, and stem cell engineering

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