Regenerating Rat Liver: Correlations Between Estrogen Receptor Localization and Deoxyribonucleic Acid Synthesis

Francavilla, A.; Leo, Alfredo Di; Eagon, Patricia K.; Wu, S. K.; Ove, Peter; Thiel, David H. Van; Starzl, Thomas E.

doi:10.1016/s0016-5085(84)80027-x

Cited by 74 publications

(32 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, a translo-cation of the estrogen receptor from the cytosolic compartment of the cell to the nuclear compartment is seen prior to the initiation of DNA synthesis. These changes suggest an important role for nuclear estrogen and particularly that an estrogen-DNA interaction exists prior to the initiation of the regenerative process within liver (15). Thus, the changes in the estrogen and androgen receptor content seen in the transplanted livers reported herein may represent a regenerative response to some transplantation-related hepatic injury.…”

Section: Discussionmentioning

confidence: 67%

Orthotopic liver transplantation and the cytosolic estrogen-androgen receptor status of the liver: The influence of the sex of the donor

et al. 1989

Self Cite

View full text Add to dashboard Cite

Mammalian liver is known to contain cytosolic receptors for both estrogens and androgens. Furthermore, certain mammalian hepatic functions are known to display a sexual dimorphism. However, in clinical liver transplantation, the sex of the donor is not taken into consideration in selection of the donor. In this study, the effect of liver transplantation on the estrogen and androgen receptor content of the liver was determined. Adult male and female rats were subjected to orthotopic liver transplantation, using donors from both the same and the opposite sex as the recipient. The animals were killed on the tenth postoperative day, and the livers were assayed to determine their cytosolic estrogen and androgen receptor content. Transplantation of a liver from a male donor into a male recipient, from a male donor into female recipient and from a female donor into a male recipient produced similar changes in the number of cytosolic estrogen and androgen receptors in hepatic cytosol. In all three situations, the estrogen receptor content in the cytosol of the transplanted liver was the same as that found in an unoperated male liver, and the cytosolic content of the androgen receptor was the same as that of an unoperated female liver. After transplantation of the liver from a female donor into a female recipient, the estrogen and androgen receptor content in the cytosol of the transplanted liver was the same as that of an unoperated female.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Section: Discussionmentioning

confidence: 67%

Orthotopic liver transplantation and the cytosolic estrogen-androgen receptor status of the liver: The influence of the sex of the donor

et al. 1989

Self Cite

View full text Add to dashboard Cite

show abstract

“…Protamine sulfate assay of cytosolic estrogen receptor-The protamine sulfate precipitate method was used to assay cytosolic estrogen receptor; this method avoids interference of [ 3 H]E 2 binding to a high-capacity male specific estrogen binder (MEB) of male rat liver cytosol (7). Cytosol and protamine sulfate precipitates were prepared as described previously (8). Protamine sulfate precipitates of 200-μl aliquots of hepatic cytosol were prepared and incubated with varying concentrations of [ 3 H]E 2 over a range of 0.15-5 nM in the absence (total binding) and presence (nonspecific binding) of 100-fold excess of unlabeled E 2 for 18 h at 0°C.…”

Section: Estrogen Binding Studiesmentioning

confidence: 99%

Circadian rhythm of hepatic cytosolic and nuclear estrogen and androgen receptors

Francavilla¹,

Eagon²,

DiLeo³

et al. 1986

Gastroenterology

Self Cite

View full text Add to dashboard Cite

Mammalian liver is a sex steroid-responsive tissue. The effects of these hormones presumably are mediated by hepatic estrogen receptors (ER) and androgen receptors (AR). Serum levels of sex hormones display circadian rhythms. Further, estrogens and androgens are commonly administered; administration of these agents is associated frequently with liver disease. Therefore, we investigated whether the cytosolic and nuclear sex steroid receptors also display a similar circadian rhythm, and whether variations occurred in the distribution of receptors between cytosolic and nuclear compartments. Animals were killed every 4 h from midnight till the following midnight; cytosolic and nuclear levels of both ER and AR were measured. Cytosolic ER reached a maximum level at 4 AM, and a minimum at 8 PM and midnight of both days. Nuclear ER was highest at 8 AM and lowest at 4 PM and 8 PM, a pattern which parallels variations in serum estradiol levels. Cytosolic AR was highest at 8 PM and lowest at midnight and 4 AM. Nuclear AR was highest at 4 AM and lowest at 4 PM and 8 PM. The highest level of nuclear AR does not correspond to the maximum serum testosterone level, which occurred at 4 PM. The total hepatic content of both ER and AR was not constant over the 24-h period, but varied considerably with time of day. These studies suggest that both ER and AR show a distinct circadian rhythm in subcellular compartmentalization, and that total hepatic content of ER and AR varies significantly during a 24-h period.Address requests for reprints to: A. Francavilla, M.D.,

show abstract

“…These included the direct regeneration indexes of DNA synthesis, labeled nuclei and subsequent mitosis (3)(4)(5). Surrogate manifestations of regeneration also were monitored: systemic insulin and glucagon; circulating sex steroid hormones and their nuclear and cytosolic liver receptors (6)(7)(8)(9)(10)(11)(12); and the expression of transforming growth factor-p (TGF-P) mRNA (13, 141, c-Ha-rus mRNA (15) and c-jun mRNA (16,17). These changes after transplantation of small-for-size livers were compared with those occurring after transplantation of size-matched livers.…”

mentioning

confidence: 99%

Small-for-size liver transplanted into larger recipient: A model of hepatic regeneration

FRANCAVILLA¹

1993

Hepatology

View full text Add to dashboard Cite

Orthotopic liver transplantation was performed in 60 recipient rats weighing 200 to 250 gm. Sixty rats of the same strain were used as liver donors, 30 weighing 100 to 140 gm (small for size) and the other 30 weighing 200 to 250 gm (same size). Mter 1, 2, 3, 4, 7 and 14 days (n = 5 each) DNA synthesis, nuclear thymidine labeling and mitoses were increased in both the smallfor-size and same-size groups, but significantly more in the former. These changes were maximal after 48 to 72 hr, similar to but later than the well-known regeneration response after partial hepatectomy, which peaks at 24 hr in rats. Indirect indexes of regeneration of the transplanted livers also were measured: plasma or serum ornithine decarboxylase; insulin and glucagon serum levels; estradiol and testosterone serum levels (and their nuclear and cytosolic receptors); and transforming growth factor-~, c-Ha-ras and c-jun mRNA expressions. With the small-for-size transplantation, these followed the same delayed pattern as the direct regeneration parameters. The small livers gradually increased in size over the course of 1 to 2 wk and achieved a volume equal to that of the liver originally present in the recipient. In contrast, no significant liver weight gain occurred in the transplanted livers from same-size donors despite the evidence of regeneration by direct indexes, but not by most of the surrogate parameters, including ornithine decarboxylase. (HEPATOLOGY 1993;19:210-216.) It has previously been demonstrated, both in human beings (1) and in dogs (2), that a liver from a small donor transplanted into a larger recipient undergoes hypertrophic and hyperplastic changes that result in a progressive increase of liver volume, until the trans-

show abstract

Regenerating Rat Liver: Correlations Between Estrogen Receptor Localization and Deoxyribonucleic Acid Synthesis

Cited by 74 publications

References 29 publications

Orthotopic liver transplantation and the cytosolic estrogen-androgen receptor status of the liver: The influence of the sex of the donor

Orthotopic liver transplantation and the cytosolic estrogen-androgen receptor status of the liver: The influence of the sex of the donor

Circadian rhythm of hepatic cytosolic and nuclear estrogen and androgen receptors

Small-for-size liver transplanted into larger recipient: A model of hepatic regeneration

Contact Info

Product

Resources

About