Regenerating Human Nasal Mucosa Cells Express Peptide Growth Factors

Hansson, Hans‐Arne; Jørgensen, F; Petruson, Björn; Petruson, Karin

doi:10.1001/archotol.1991.01870240060010

Cited by 23 publications

(21 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…goblet cell hyperplasia (32), might confer them with enough protection so there is no severe damage to the epithelium (38). Age-dependent differences in the metabolism of xenobiotics, plasma-derived molecules playing a role in the mucosal repair process, peptide growth factors, cell migration, DNA repair and epithelial regeneration may explain the SWMMC children's nasal cell turnover (2,3,5,(39)(40)(41)(42).…”

Section: Discussionmentioning

confidence: 99%

Cell proliferation in nasal respiratory epithelium of people exposed to urbanpollution

Calderón‐Garcidueñas¹,

Rodrı́guez-Alcaraz²,

Garcı́a³

et al. 1999

Carcinogenesis

View full text Add to dashboard Cite

The nasal passages are a common portal of entry and are a prime site for toxicant-induced pathology. Sustained increases in regenerative cell proliferation can be a significant driving force in chemical carcinogenesis. The atmosphere in Mexico City contains a complex mixture of air pollutants and its residents are exposed chronically and sequentially to numerous toxicants and potential carcinogens. We were concerned that exposure to Mexico City's atmosphere might induce cytotoxicity and increase nasal respiratory epithelial cell proliferation. Nasal biopsies were obtained for DNA cell cycle analysis from 195 volunteers. The control population consisted of 16 adults and 27 children that were residents in a Caribbean island with low pollution. The exposed Mexico City population consisted of 109 adults and 43 children. Sixty-one of the adult subjects were newly arrived in Mexico City and were followed for 25 days from their arrival. Control children, control adult and exposed Mexico City children all had similar percentages of cells in the replicative DNA synthesis phase (S phase) of the cell cycle (%S). A significant increase in %S in nasal epithelial cells was seen in exposed adult residents in Mexico City biopsied at three different dates compared with control adults. Newly arrived adults exhibited a control level of cell turnover at day 2 after coming to the city. However, at days 7, 14 and 25 they exhibited significant increases in %S. These data demonstrate an increased and sustained nasal cell turnover rate in the adult population observable in as little as 1 week of residence in Mexico City. This increase in cell proliferation is in agreement with other reports of induced pathological changes in the nasal passages of Mexico City dwellers. These observations suggest an increased potential risk factor of developing nasal neoplasms for residents of large cities with heavy pollution.

show abstract

Section: Discussionmentioning

confidence: 99%

Cell proliferation in nasal respiratory epithelium of people exposed to urbanpollution

Calderón‐Garcidueñas¹,

Rodrı́guez-Alcaraz²,

Garcı́a³

et al. 1999

Carcinogenesis

View full text Add to dashboard Cite

show abstract

“…Fibroblasts, which are abundant in the lamina propria underneath the olfactory epithelium, could provide neurotrophic support (Hansson et al, 1991). To test the effects of fibroblasts and to also test the trophic support of a tissue unrelated to the brain or nose, fibroblasts (FIBRO) were purified from newborn rat belly skin, cocultured with ANM cells on a cortical glia feeder layer (FIBRO/ ANM), and OMPϩ numbers were counted at 9 and 12 DIV (Fig.…”

Section: Rat Skin Fibroblasts Did Not Support Osn Survivalmentioning

confidence: 99%

In VitroGeneration of Adult Rat Olfactory Sensory Neurons and Regulation of Maturation by Coculture with CNS Tissues

Grill

Pixley

1997

J. Neurosci.

View full text Add to dashboard Cite

Olfactory sensory neurons (OSNs) are continually generated throughout life. Although previous studies have examined neurogenesis in olfactory cell cultures derived from embryonic or newborn rodents, we demonstrate neurogenesis in cell cultures derived from adult rat tissues. Dissociated cells taken from adult rat nasal mucosal tissues (ANM cells) were plated onto a feeder layer of newborn rat cortical glia (astrocytes) in serumfree conditions. Immature OSNs (stained for neuron-specific tubulin, NST) increased in number between 1 and 5 d in vitro (DIV) and in mass thereafter. Mature OSN (stained for olfactory marker protein, OMP) numbers decreased between 1 and 5 DIV, then increased over 5 DIV values by 12 and 15 DIV. Pulse labeling with [ 3 H]thymidine confirmed in vitro neurogenesis. To determine whether the target cells for OSNs, olfactory bulb (OB) neurons, provide trophic support, dissociated newborn rat OB cells were cocultured with ANM cells on glia. This resulted in greater numbers of OMP-positive (OMPϩ) neurons after 9 DIV than ANM-alone cultures. This neurotrophic effect was not OB specific. Addition of newborn rat cerebellar and embryonic rat ventral mesencephalic cells to ANM cells also increased OMPϩ neurons, whereas addition of newborn rat cortical cells or controls (purified glia or fibroblasts) did not. Changes in numbers of dopaminergic neurons (stained for tyrosine hydroxylase), present in OB and VM cultures, did not correlate with OMPϩ neuronal increases. Thus, cultures of adult rat OSNs demonstrate neurogenesis, and trophic/maturation support is variably provided by CNS neurons (and not glia).

show abstract

“…7 In their study, immunohistochemical investigation of biopsy specimens of healthy human nasal mucosa (from subjects aged 6 to 72 years) showed that intense concentrations of insulin-like growth factor 1 were demonstrable in areas with deficient epithelial lining and inflammatory cells. Levels of this latter hormone are also known to rise during the course of pregnancy.…”

Section: Discussionmentioning

confidence: 98%