Regenerating cellulose from ionic liquids for an accelerated enzymatic hydrolysis

Zhao, Hua; Jones, Cecil L.; Baker, Gary A.; Xia, Shuqian; Olubajo, Olarongbe; Person, Vernecia

doi:10.1016/j.jbiotec.2008.08.009

Cited by 439 publications

(283 citation statements)

References 45 publications

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“…To select the IL which can conduct cellulase compatability, cellulase activity and enzymatic saccharification of untreated corn cob in the presence of 20% (v/v) Recently, two kinds of processes were developed in ionic liquid-involved cellulosic material pretreatment and saccharification process: pretreatment/saccharification process (Dadi et al 2006;Zhao et al 2009) and in situ saccharification process (Kamiya et al 2008). In our case, saccharification of corn cob was carried out by the two kinds of processes to evaluate [Mmim]DMP pretreatment on saccharification.…”

Section: Selection Of Green Cellulase-friendly Ilsmentioning

confidence: 99%

“…Furthermore, increased rate of cellulose hydrolysis via cellulase in ionic liquids could lead to increased production of fermentable sugars that can be converted into fuels. In addition, ionic liquids involved processes are less energy demanding, easier to operate, and more environmentally friendly than current dissolution processes (Zhao et al 2009;Rogers and Seddon 2003;Hélène and Lionel 2002).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corn cob for improved saccharification

Jiang

et al. 2010

Appl Microbiol Biotechnol

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Ionic liquid (IL) pretreatment of lignocellulose materials is a promising process in biomass conversion to renewable biofuel. More in-depth research involving environment-friendly IL is much needed to explore pretreatment green route. In our case, IL 1-methyl-3-methylimidazolium dimethylphosphite ([Mmim]DMP) was chosen as an environment-friendly solvent to pretreat corn cob in view of its biocompatibility with both lignocellulose solubility and cellulase activity. The pretreatment/saccharification process and in situ saccharification process involving [Mmim]DMP were efficiently performed in bioconversion of corn cob to sugars, and more than 70% saccharification rates were obtained. Furthermore, the fermentability of reducing sugars obtained from the hydrolyzates was evaluated using Rhodococcus opacus strain ACCC41043 (R. opacus). High lipid production 41-43% of cell dry matter was obtained after 30 h of cultivation. GC/ MS analysis indicated that lipids from R. opacus contained mainly long-chain fatty acids with four major constituent/ oleic acid, stearic acid, palmitic acid, palmitoleic acid which are good candidates for biodiesel. These elucidated that corn cob pretreated by IL [Mmim]DMP did not bring negative effects on saccharification, cell growth, and accumulation of lipid of R. opacus. In conclusion, the IL [Mmim]DMP shows promise as green pretreatment solvent for cellulosic materials.

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Section: Selection Of Green Cellulase-friendly Ilsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corn cob for improved saccharification

Jiang

et al. 2010

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

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“…A reduction in crystallinity was observed after IL pretreatment of switchgrass, maple wood, yellow pine, and hardwood red oak (Lee et al 2009;Li et al 2010;Sun et al 2009). Moreover, in IL pretreated biomass samples, cellulose can be easily recovered with the addition of anti-solvents, such as water, methanol, ethanol, and acetone, and the enzymatic hydrolysis of the recovered cellulose is much greater than that of untreated cellulose (da Costa Lopes et al 2013;Dadi et al 2006;Lee et al 2009;Li et al 2009;Singh et al 2009;Sun et al 2009;Zhao et al 2009a). …”

Section: Introductionmentioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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Chemical and physical pretreatment of biomass is a critical step in the conversion of lignocellulose to biofuels and bioproducts. Ionic liquid (IL) pretreatment has attracted significant attention due to the unique ability of certain ILs to solubilize some or all components of the plant cell wall. However, these ILs not only inhibit enzyme activities, but also the growth and productivity of microorganisms used in downstream hydrolysis and fermentation processes.While pretreated biomass can be washed to remove residual IL and reduce inhibition, extensive washing is costly and not feasible in large-scale processes. IL tolerant microorganisms and microbial communities have been discovered from environmental samples and studies begun to elucidate mechanisms of IL tolerance. The discovery of IL tolerance in environmental microbial communities and individual microbes has lead to the proposal of molecular mechanisms of resistance. In this article, we review recent progress on discovering IL tolerant microorganisms, identifying metabolic pathways and mechanisms of tolerance, and engineering microorganisms for IL tolerance. Research in these areas will yield new approaches to overcome inhibition in lignocellulosic biomass bioconversion processes and increase opportunities for the use of ILs in biomass pretreatment.

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“…Imidazolium-and halogen-based ILs were reported to decrease the cellulase activity (Bose et al 2012;Li et al 2013). Even trace amounts of these ILs may worsen significantly the cellulase activity (Zhao et al 2009) and this makes an extensive removal necessary for the residual IL before enzymatic conversion (Datta et al 2010;Shi et al 2013). This seems to be not the case in the experiments in the present paper, where only a simple regeneration and washing processes was applied.…”

Section: Effect Of Aail/cosolvent Pretreatment On Enzymatic Hydrolysismentioning

confidence: 67%

Novel cellulose pretreatment solvent: phosphonium-based amino acid ionic liquid/cosolvent for enhanced enzymatic hydrolysis

et al. 2016

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Abstract:The potential of halogen-free and imidazoliumfree phosphonium-based amino acid ionic liquids (AAILs) has been investigated as new solvents for cellulose pretreatment for the subsequent enzymatic hydrolysis of cellulose. AAILs alone did not dissolve cellulose (Avicel), even at 120°C. However, when polar solvents such as dimethylsulfoxide (DMSO) were added as cosolvents, AAILs became an acceptable solvent for cellulose at 30°C. The solubility of cellulose in tetrabutylphosphonium glycine ([TBP][Gly])/cosolvent reached 15%. The enzymatic hydrolysis of cellulose was dramatically enhanced by pretreatment with AAIL/cosolvent, and the glucose yield reached 100% when the novel AAIL tetrabutylphosphonium N,N-dimethylglycine ([TBP][DMGly]) was used in combination with DMSO as cosolvent. The enzymatic conversion of cellulose to glucose in 6% and 13% [TBP] [DMGly]/DMSO buffer solutions reached 98% and 79%, respectively. The decrease in cellulase activity owing to residual [TBP][DMGly]/DMSO was not significant. Hence, it is possible to conduct the dissolution and enzymatic hydrolysis of cellulose in a one-batch process in a phosphonium-based AAIL/cosolvent system.

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Regenerating cellulose from ionic liquids for an accelerated enzymatic hydrolysis

Cited by 439 publications

References 45 publications

Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corn cob for improved saccharification

Evaluation of the biocompatibile ionic liquid 1-methyl-3-methylimidazolium dimethylphosphite pretreatment of corn cob for improved saccharification

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Novel cellulose pretreatment solvent: phosphonium-based amino acid ionic liquid/cosolvent for enhanced enzymatic hydrolysis

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