Regenerated silk fibroin (RSF) electrostatic spun fibre composite with polypropylene mesh for reconstruction of abdominal wall defects in a rat model

Yang, Dongchao; Song, Zhicheng; Shen, Jiali; Song, Heng; Yang, Jianjun; Zhang, Peihua; Gu, Yu

doi:10.1080/21691401.2019.1709858

Cited by 19 publications

(16 citation statements)

References 34 publications

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“…These phenomena are important features of abdominal wall repair. 6 The adhesions between graft and viscera in the experiment group were obviously lower than that in the control group (Figure 8). This may be caused by the composite hydrogel contained in P-ADM.…”

Section: Discussionmentioning

confidence: 87%

“…3,4 However, prostheses are permanent foreign materials in the body and can cause chronic inflammation, fibrosis, and other complications. 5,6 To overcome these challenges, biomaterials derived from animal sources have been explored and show potential for the surgical repair of these abdominal wall defects. 7 Although in clinical surgical practice synthetic mesh is preferred due to its strength and ingrowth, the abovementioned complications limit its further application.…”

Section: Introductionmentioning

confidence: 99%

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A facile strategy for fabricating composite patch scaffold by using porcine acellular dermal matrix and gelatin for the reconstruction of abdominal wall defects

Liu

Zhang

et al. 2020

J Biomater Appl

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Defects of the abdominal wall continue to be a significant issue in regenerative medicine and tissue engineering. Porcine acellular dermal matrix (P-ADM) and gelatin have been widely used for fabricating engineered abdominal wall due to their good biocompatibility and integration with host tissues. In this study, we demonstrate a novel and reproducible approach of using gelatin and P-ADM powder to fabricate composite patch scaffolds by combining solvent casting and enzymatic cross-linking for the abdominal wall regeneration in rats. Different concentration ratios of gelatin and P-ADM and different shapes of engineered scaffolds are studied for various abdominal wall defects. Skeletal muscle cells and human umbilical vein endothelial cells can successfully attach to the surface of the scaffolds and maintain high viability after culturing for five days. In vivo experiments demonstrate that there are no signs of infection, intra-abdominal adhesion, and severe inflammation after two weeks, which indicate good repairability of the scaffolds. In conclusion, we propose a novel method that holds great potential for building engineered scaffolds to meet the different requirements of abdominal wall regeneration.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

A facile strategy for fabricating composite patch scaffold by using porcine acellular dermal matrix and gelatin for the reconstruction of abdominal wall defects

Liu

Zhang

et al. 2020

J Biomater Appl

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“…Since the artificial mesh with a single component cannot meet the clinical requirements for incisional hernia repair of the abdominal wall, the composite mesh has made great progress and is mainly used to reduce the incidence and degree of adhesion after surgery so as to reduce the harm of adhesion complications. However, it has been reported recently that the composite mesh of synthetic materials may cause postoperative complications, such as intestinal obstruction, enterocutaneous fistulas, and pain ( Yang et al, 2020b ; Devin et al, 2021 ). Also, its degradation products may cause inflammation, erosion, and foreign body reactions.…”

Section: Discussionmentioning

confidence: 99%

“…Although the use of mesh greatly reduces the recurrence rate of incisional hernia and promotes wound healing, according to a large number of clinical data feedback, the polyester mesh is rarely used due to poor anti-infection ability and heavy foreign body reaction. The polypropylene mesh has good histocompatibility, light inflammatory response, and strong anti-infection ability, but it is easy to adhere to viscera, resulting in intestinal obstruction, intestinal leakage, and other complications ( Aydemir Sezer et al, 2019 ; Yang et al, 2020b ; Nessel et al, 2021 ; Pascual et al, 2022 ). Expanded polytetrafluoroethylene (ePTFE) is not easy to adhere to viscera, but it has poor anti-infection ability and poor firmness.…”

Section: Introductionmentioning

confidence: 99%

Construction and properties of the silk fibroin and polypropylene composite biological mesh for abdominal incisional hernia repair

Luan

Cao

et al. 2022

Front. Bioeng. Biotechnol.

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Background: In this study, a new composite biological mesh named SFP was prepared by combining silk fibroin with polypropylene mesh. The mechanism and clinical application value of the SFP composite mesh were explored.Methods: The fibrous membrane was prepared by electrospinning of silk fibroin. The silk fibrous membrane was adhered to the polypropylene mesh by fibrin hydrogel to make a new composite mesh. The characterizations were verified by structural analysis and in vitro cell experiments. A total of 40 Sprague–Dawley rats were randomly divided into two groups, and 20 rats in each group were implanted with the SFP mesh and pure polypropylene mesh, respectively. The rats were sacrificed in batches on the 3rd, 7th, 14th, and 90th days after surgery. The adhesion degree and adhesion area on the mesh surface were compared, and a histopathological examination was carried out.Results:In vitro cell function experiments confirmed that the SFP mesh had good cell viability. The control group had different degrees of adhesion on the 3rd, 7th, 14th, and 90th days after surgery. However, there was almost no intraperitoneal adhesions on the 3rd and 7th days after surgery, and some rats only had mild adhesions on the 14th and 90th days after surgery in the SFP group. There were statistically significant differences in the postoperative intraperitoneal adhesion area and adhesion degree between the two groups (p < 0.05). Histopathological examination confirmed that the mesenchymal cells were well arranged and continuous, and there were more new capillaries and adipocyte proliferation under the mesenchymal cells in the SFP group.Conclusion: The SFP mesh shows good biocompatibility and biofunction in vitro and in vivo. It can promote the growth of peritoneal mesenchymal cells. The formation of a new mesenchymal cell layer can effectively reduce the extent and scope of adhesion between the mesh and abdominal organs. The SFP mesh will have a good application prospect in the field of abdominal wall hernia repair.

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“…Comparing the PCL coatings and uncoated PP meshes, the RSF coating prevented abdominal adhesions and effectively reduced inflammation. [ 221 ]…”

Section: Inhibitory Biological Process For Anti‐adhesionmentioning

confidence: 99%

Two Sides of Electrospun Fiber in Promoting and Inhibiting Biomedical Processes

Wang

Cui

2020

Advanced Therapeutics

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Electrospinning is a versatile technique for generating ultrathin fibers, which is widely applied in various biomedical applications. The properties and structures of the fibers are specifically engineered to meet the needs of particular applications. The impact of nanofibrous scaffolds on biological processes is related to the promotion or inhibition of specific cell or bioactive substance functions. Here, a novel analysis of the diverse roles of electrospun nanofibrous scaffolds is provided and recent advances in exploiting their binary attributes for applications in biomedicine are summarized. This review initially introduces the development techniques for electrospinning, specifically the engineering of electrospun nanofibers. Then, scaffold applications for cell migration, adhesion, proliferation, and accelerating regeneration in cardiac, nerve, skeletal muscle, bone tissue, and wound healing are presented. Moreover, their inhibitory properties in cancer treatments, antibacterial applications, anti‐adhesion, anti‐scarring, and inhibition of thrombus formation are summarized. Eventually, the potential for future work using multifunctional, electrospun, nanofibrous scaffolds, in order to further inspire the development of scaffolds for biomedical treatments is summarized and discussed.

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Regenerated silk fibroin (RSF) electrostatic spun fibre composite with polypropylene mesh for reconstruction of abdominal wall defects in a rat model

Abstract: Gu (2020) Regenerated silk fibroin (RSF) electrostatic spun fibre composite with polypropylene mesh for reconstruction of abdominal wall defects in a rat model, Artificial Cells,

Cited by 19 publications

References 34 publications

A facile strategy for fabricating composite patch scaffold by using porcine acellular dermal matrix and gelatin for the reconstruction of abdominal wall defects

A facile strategy for fabricating composite patch scaffold by using porcine acellular dermal matrix and gelatin for the reconstruction of abdominal wall defects

Construction and properties of the silk fibroin and polypropylene composite biological mesh for abdominal incisional hernia repair

Two Sides of Electrospun Fiber in Promoting and Inhibiting Biomedical Processes

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