Regenerated protein fibers. II. Viscoelastic behavior of silk fibroin solutions

Matsumoto, Kiyoichi; Uejima, Hiroyuki; Sano, Yoshiyuki; Sumino, Hiroshi

doi:10.1002/(sici)1099-0518(19970730)35:10<1955::aid-pola10>3.0.co;2-g

Cited by 8 publications

(4 citation statements)

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“…It was observed that the half-life of the SF solution could be increased from 3 weeks to more than 6 months if the solution is stored at 4 • C without removing LiBr. Removal of LiBr could allow the interchain hydrophobic interactions between the SF molecules and lead to the formation of hydrogen-bonded βsheets, which eventually convert the solution to gel and finally solid form [56,57]. The presence of LiBr in the SF solution was also crucial for the successful methacrylation of SF because the agitation during the reaction without LiBr may result in the shear-induced unfolding of the SF molecules.…”

Section: Extraction Of Sf Fibers and Preparation Of Sf Solutionmentioning

confidence: 99%

“…The presence of LiBr in the SF solution was also crucial for the successful methacrylation of SF because the agitation during the reaction without LiBr may result in the shear-induced unfolding of the SF molecules. The increased exposure of the hydrophobic domains and hence the formation of β-sheet clusters can eventually lead to physical entanglement of fibroin fibers resulting in aggregation and gelation of SF [56,57].…”

Section: Extraction Of Sf Fibers and Preparation Of Sf Solutionmentioning

confidence: 99%

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Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Sundaran,

Kok,

Chang

2023

Biomed. Mater.

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An optimal wound-healing hydrogel requires effective antibacterial properties and a favorable cell adhesion and proliferation environment. Although Bombyx mori silk fibroin (SF) possesses inherent wound-healing properties, it lacks these essential qualities. This study aimed to fabricate a novel photo-polymerizable hydrogel by utilizing SF's wound-healing efficiency and the epsilon-poly-L-lysine (EPL) antimicrobial activity. The SF was modified with three different concentrations of glycidyl methacrylate (GMA) to obtain SF-GMA(L), SF-GMA(M), and SF-GMA(H). A methacrylated EPL (EPL-GMA) was also produced. Then, SF-GMA was mixed with EPL-GMA to produce photo-crosslinkable SF-GMA-EPL hydrogels. The SF-GMA(L)-EPL, SF-GMA(M)-EPL, and SF-GMA(H)-EPL hydrogels, fabricated with 20% EPL-GMA, demonstrated maximum antimicrobial activity and mammalian cell adhesion ability. The hydroxyl radical (•OH) scavenging efficiency of the hydrogels was tested and shown to be between 69% and 74%. These hydrogels also exhibited 60% efficiency in removing bacterial lipopolysaccharides. The water absorption ability of the hydrogels was consistent with the size of their internal pores. The hydrogels exhibited a slow degradation fashion, and their degradation products appeared cytocompatible. Finally, the elastomeric properties of the hydrogels were determined, and a storage modulus (G') of 300–600 Pa was demonstrated. In conclusion, the hydrogels created in this study possess excellent biological and physical properties to support wound healing.

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Section: Extraction Of Sf Fibers and Preparation Of Sf Solutionmentioning

confidence: 99%

Section: Extraction Of Sf Fibers and Preparation Of Sf Solutionmentioning

confidence: 99%

Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Sundaran,

Kok,

Chang

2023

Biomed. Mater.

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“…The LiBr-ethanol-water system demonstrates a rapid dissolution rate and high yield. However, the viscoelasticity of the obtained solution is proportional to the concentration of RSF and the ratio of ethanol to water in the solvent [169].…”

Section: Salt-alcohol-water Systemmentioning

confidence: 99%

Advances in Preparation and Properties of Regenerated Silk Fibroin

Huang,

Shi,

Zhou

et al. 2023

IJMS

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Over the years, silk fibroin (SF) has gained significant attention in various fields, such as biomedicine, tissue engineering, food processing, photochemistry, and biosensing, owing to its remarkable biocompatibility, machinability, and chemical modifiability. The process of obtaining regenerated silk fibroin (RSF) involves degumming, dissolving, dialysis, and centrifugation. RSF can be further fabricated into films, sponges, microspheres, gels, nanofibers, and other forms. It is now understood that the dissolution method selected greatly impacts the molecular weight distribution and structure of RSF, consequently influencing its subsequent processing and application. This study comprehensively explores and summarizes different dissolution methods of SF while examining their effects on the structure and performance of RSF. The findings presented herein aim to provide valuable insights and references for researchers and practitioners interested in utilizing RSF in diverse fields.

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“…Preparo e avaliação comparativa das propriedades físico-químicas de hidrogéis de fibroína de seda com conteúdo variado de sericina obtidos a partir dos cloretos de cálcio e lítio em sistemas distintos de solventes Natália Marchesan Bexiga 2 A sericina exerce a função de manter os fios de fibroína unidos, e está associada à resposta imunológica do Bombyx mori, isto é, possui a função de proteção do casulo durante o período de crescimento (WENK et al, 2010;ALTMAN et al, 2003). et al, 1990;LI et al, 2001;MATHUR et al, 1997;MATSUMOTO et al, 1997;AGARVAL et al, 1997;SUN et al, 1997).…”

Section: Ficha Catalográficaunclassified

Preparo e avaliação comparativa das propriedades físico-químicas de hidrogéis de fibroína de seda com conteúdo variado de sericina obtidos a partir dos cloretos de cálcio e lítio em sistemas distintos de solventes

Bexiga¹

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Preparo e avaliação comparativa das propriedades físicoquímicas de hidrogéis de fibroína de seda com conteúdo variado de sericina obtidos a partir dos cloretos de cálcio e lítio em sistemas distintos de solventes Natália Marchesan Bexiga Dissertação para obtenção do grau de MESTRE Orientador: Prof. Dr. Bronislaw Polakiewicz São Paulo 2014 Prof. Dr. Bronislaw Polakiewicz orientador/presidente ____________________________ 1° Examinador ____________________________ 2° Examinador São Paulo, __________ de ______. iii AGRADECIMENTOS Ao meu orientador Professor Bronislaw Polakwiecz, pela confiança, compreensão, auxílio e orientação durante todo o decorrer do trabalho. Ao professor Adalberto Pessoa Jr por todo o auxílio e incentivo. Aos professores do departamento de Tecnologia Bioquímico-Farmacêutico pelo incentivo e colaboração. À amiga Adriana Maia Bezerra, por todo companheirismo, paciência, dedicação e incentivos constantes. À amiga Mariana Agostini de Moraes, por todo auxílio e amizade. Ao amigo Marcos Camargo Knirsch, pelo apoio incondicional. Aos funcionários do departamento, por toda ajuda. Aos amigos e companheiros de laboratório, pela amizade e companheirismo. Ao meu pai João Roberto Bexiga, que sempre me apoiou e acreditou no meu potencial. 4.3.1 ENSAIOS DE TEXTURA 49 4.3.2 ENSAIOS REOLÓGICOS 49 4.6 PREPARO DOS HIDROGÉIS 50 4.7 CARACTERIZAÇÃO DOS HIDROGÉIS 51 4.7.1 TERMOGRAVIMETRIA (TGA) 51 v 4.7.2 DIFRAÇÃO DE RAIOS -X (DRX) 51 4.7.3 INFRAVERMELHO (FTIR) 52 4.7.4 MICROSCOPIA ELETRÔNICA DE VARREDURA (MEV) 52 5 RESULTADOS E DISCUSSÃO 53 5.1 REMOÇÃO DA SERICINA 54 5.2 PREPARO DAS DISPERSÕES DE FIBROÍNA 55 5.3 CARACTERIZAÇÃO DAS DISPERSÕES 61 5.3.1 ENSAIOS DE TEXTURA 62 5.3.2 ENSAIOS DE REOLOGIA 63 5.4 PREPARO DOS HIDROGÉIS 72 5.5 CARACTERIZAÇÃO DOS HIDROGÉIS 78 5.5.1 TERMOGRAVIMETRIA (TGA) 79 5.5.2 DIFRAÇÃO DE RAIOS-X (DRX) 86 5.5.3 INFRAVERMELHO (FTIR) 94 5.5.4 MICROSCOPIA ELETRÔNICA DE VARREDURA (MEV) 101 6 CONCLUSÂO 102 REFERÊNCIAS BIOBLIOGRÁFICAS 114 ANEXOS 122 APÊNDICE 65

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Regenerated protein fibers. II. Viscoelastic behavior of silk fibroin solutions

Cited by 8 publications

References 0 publications

Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Fabrication and in vitro evaluation of photo cross-linkable silk fibroin–epsilon-poly-L-lysine hydrogel for wound repair

Advances in Preparation and Properties of Regenerated Silk Fibroin

Preparo e avaliação comparativa das propriedades físico-químicas de hidrogéis de fibroína de seda com conteúdo variado de sericina obtidos a partir dos cloretos de cálcio e lítio em sistemas distintos de solventes

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