Regenerated Cellulose Fiber and Film Immobilized with Lysozyme

Abstract

The present work reports an initial engineering approach for fabricating lysozyme-bound regenerated cellulose fiber and film. Glycine-esterified cotton was dissolved in an ionic liquid solvent 1-Butyl-3-methylimidazolium Chloride (BMIMCl) in which lysozyme was activated and covalently attached to cotton cellulose through an enzymatic conjugation between its carboxyl groups and glycine cellulose's amino groups. The resulting solution was extruded for fiber/film formation in a water bath. After performing a bicinchoninic acid (BCA) protein assay, quantity of attached lysozyme to cellulose fiber/film was evaluated. The study exhibited that a synthesis of lysozyme conjugation on cellulose in BMIMCl could be completed in a control manor, resulting in a cellulose solution suitable for fiber/film production. It was also found that lysozyme could be successfully immobilized onto the cellulose fiber and film regenerated from solution spinning with a reasonable amount ranging from 197.6 to 343.7 μg/mL.mg.

Enzyme immobilization on cellulose matrixes

“…Esterification is one of the common and easy methods to introduce the amino groups onto cellulose surface. [51][52][53] Taking glycine for instance, as shown in Figure 2(a), a carboxyl group of glycine reacts with a hydroxyl group of cellulose to introduce an amino group on cellulose. Then, the amino group is able to couple with a carboxyl group of enzymes.…”