Regarding “randomized trial and local biological effect of autologous platelets used as adjuvant therapy for chronic venous leg ulcers”

Borzini, P; Mazzucco, Laura; Panizza, Renzo; Rivara, Giorgio

doi:10.1016/j.jvs.2004.01.047

Cited by 9 publications

(20 citation statements)

References 2 publications

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“…10 In other reports, no detail on leukodepletion was given. 6,8,[11][12][13][14][15] Human PLTs were used thoroughly with a unique exception where equine PLTs were used. 11 In most studies, no detail was given on donor selection criteria.…”

Section: Plt Preparationmentioning

confidence: 99%

“…12 In one study, PLTs were collected from both donors and patients. 13 The number of subjects who were actually investigated was reported only in two studies, 213 and 5, respectively. 6,8 The concentration of the PLT suspension was reported in all cases but one.…”

Section: Plt Preparationmentioning

confidence: 99%

“…Most authors have used PLTs at the concentration of 1 ¥ 10 9 to 2 ¥ 10 9 per mL. 6,8,10,13,14 Others have used PLTs at a lower concentration, ranging from 1.0 ¥ 10 6 to 6.53 ¥ 10 8 per mL. 9,11,12 In a single case, instead of PLT concentration, the authors described the protein content of the PLT releasate.…”

Section: Plt Preparationmentioning

confidence: 99%

“…12 In two cases, washed PLTs (phosphate-buffered saline and PBS-ethylenediaminetetraacetate, respectively) were used. 9,13 One report described the use of 4 percent paraformaldehyde-fixed PLTs. 9 Frozen PLTs (from -30∞C to -80∞C) were used by several authors.…”

Section: Plt Preparationmentioning

confidence: 99%

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Tissue regeneration and in loco administration of platelet derivatives: clinical outcome, heterogeneous products, and heterogeneity of the effector mechanisms

Borzini

Mazzucco

2005

Transfusion

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So many variables were found making standardization or confrontation of the in vitro and the in vivo studies barely conceivable or manageable. The mechanisms of action are very complex. The attribution of tissue regeneration capacity of PLT derivatives solely to the PLT-derived growth factors is simplistic. The results obtained through in vitro experiments are indicative for general mechanisms. Their simplistic hold to the complex in vivo environment may be misleading.

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Section: Plt Preparationmentioning

confidence: 99%

Section: Plt Preparationmentioning

confidence: 99%

Section: Plt Preparationmentioning

confidence: 99%

Section: Plt Preparationmentioning

confidence: 99%

See 2 more Smart Citations

Tissue regeneration and in loco administration of platelet derivatives: clinical outcome, heterogeneous products, and heterogeneity of the effector mechanisms

Borzini

Mazzucco

2005

Transfusion

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“…These platelet preparations have been applied in diverse clinical fields. They have been used locally by ophthalmologists as an adjuvant to the ophthalmologic treatment of macular holes [10,11]; they have been used in cases of bone grafting showing to promote bone formation [12,13]; and they have proved effective in healing of chronic venous ulcers in cases of vascular surgery and dermatology [14,15]. Platelet concentrates has also been found to enhance healing of various kinds of wounds [16,17].…”

Section: Discussionmentioning

confidence: 99%

Determining the Effect of Preparation and Storage: An Effort to Streamline Platelet Components as a Source of Growth Factors for Clinical Application

Sonker

Dubey

2015

Transfus Med Hemother

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Background: In the present study, different methods for preparation of platelet-rich plasma (PRP) are investigated in order to standardize the component in terms of growth factor content. The effects of concentration technique and storage duration are also analyzed. Methods: PRP was collected from 40 donors by plateletpheresis as well as by the buffy coat and tube method. Concentration of growth factors was performed using double freeze thaw- and CaCl₂-induced degranulation techniques. Growth factor estimation was performed using ELISA. Results: The levels of growth factors were highest in PRP from buffy coat, moderately lower in plasma gained by plateletpheresis and lowest in that obtained by the tube method. Mean levels of platelet-derived growth factors (PDGF) AB and BB are significantly higher when CaCl₂ was used for concentrating the growth factors. The mean levels of transforming growth factor β1 and insulin-like growth factor I were higher when applying the double freeze thaw technique. There was a substantial decline in the levels of growth factors during storage. Conclusion: The buffy coat method is suitable as preparation method for PRP in most settings. The double freeze thaw technique is better suited as concentration technique as it causes lysis of both platelets and white blood cells for releasing growth factors and is easier to perform. Growth factors are not stable in plasma, thus PRP should be frozen immediately after preparation.

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Leucocyte-Platelet Haemocomponents for Topical Use: Regenerative Potentiality

Caloprisco¹,

Borean²

2010

Advances in Minimally Invasive Surgery and Therapy for Spine and Nerves

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Wider and wider is the interest in different clinical ambits in the application of haemocomponents produced from peripheral blood for regenerative purposes. These are mainly made up of concentrated platelets capable of releasing locally growth factor (GF) that stimulates tissue regeneration. Our group has devised a method to produce a new leucocyte-platelet haemocomponent enriched in fibrinogen that integrates the GF stimulus with the presence of cells involved in the regenerative processes: monocytes and stem cells. The use of the cell separator to collect these haemocomponents from peripheral blood has allowed us to realize a safe standardized product, with good regenerative potentiality and reasonable costs. This is obtained by modifying some parameters of separation, and without cell manipulation.

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Regarding “randomized trial and local biological effect of autologous platelets used as adjuvant therapy for chronic venous leg ulcers”

Cited by 9 publications

References 2 publications

Tissue regeneration and in loco administration of platelet derivatives: clinical outcome, heterogeneous products, and heterogeneity of the effector mechanisms

Tissue regeneration and in loco administration of platelet derivatives: clinical outcome, heterogeneous products, and heterogeneity of the effector mechanisms

Determining the Effect of Preparation and Storage: An Effort to Streamline Platelet Components as a Source of Growth Factors for Clinical Application

Leucocyte-Platelet Haemocomponents for Topical Use: Regenerative Potentiality

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