Refractive indices of wheat starch granules at various moisture levels determined with an interference microscope

Wolf, Martine; Ruggles, Veronica J.; Macmasters, M. M.

doi:10.1016/0006-3002(62)91089-2

Cited by 18 publications

(11 citation statements)

References 5 publications

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“…Starch granules were detected using a flow cytometer. Moist starch granules have a refractive index of 1.52 ( 68 ). Here, we use a CytoFlex [settings: forward scatter (FSC) gain, 20; threshold FSC, 6000; max, 3000 events s −1 ; λ = 488 nm] to measure 50,000 granules per sample.…”

Section: Methodsmentioning

confidence: 99%

Accelerated ex situ breeding of GBSS - and PTST1 -edited cassava for modified starch

et al. 2018

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Section: Methodsmentioning

confidence: 99%

Accelerated ex situ breeding of GBSS - and PTST1 -edited cassava for modified starch

et al. 2018

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“…Materials and Methods, three tables, five figures, and references (12,33,50) are available at http://www.biophysj.org/biophysj/supplemental//S0006-3495(12) 00801-6.…”

Section: Supporting Materialsmentioning

confidence: 99%

Cell-to-Cell Diversity in a Synchronized Chlamydomonas Culture As Revealed by Single-Cell Analyses

Garz

Sandmann

Rading

et al. 2012

Biophysical Journal

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In a synchronized photoautotrophic culture of Chlamydomonas reinhardtii, cell size, cell number, and the averaged starch content were determined throughout the light-dark cycle. For single-cell analyses, the relative cellular starch was quantified by measuring the second harmonic generation (SHG). In destained cells, amylopectin essentially represents the only biophotonic structure. As revealed by various validation procedures, SHG signal intensities are a reliable relative measure of the cellular starch content. During photosynthesis-driven starch biosynthesis, synchronized Chlamydomonas cells possess an unexpected cell-to-cell diversity both in size and starch content, but the starch-related heterogeneity largely exceeds that of size. The cellular volume, starch content, and amount of starch/cell volume obey lognormal distributions. Starch degradation was initiated by inhibiting the photosynthetic electron transport in illuminated cells or by darkening. Under both conditions, the averaged rate of starch degradation is almost constant, but it is higher in illuminated than in darkened cells. At the single-cell level, rates of starch degradation largely differ but are unrelated to the initial cellular starch content. A rate equation describing the cellular starch degradation is presented. SHG-based three-dimensional reconstructions of Chlamydomonas cells containing starch granules are shown.

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“…This amount of water (approximately 11%) in the starch grain is significant in several aspects of starch behavior. It is the limiting value of hydration with no change in the refractive index of wheat starch (Wolf et al 1962); it is the point at which the negative standard differential entropy and differential enthalpy of adsorption are at a maximum in B potato starch (Macchia and Bettelheim 1964;Morsi et al 1967); and it is the value at which the intensity of X-ray reflections reaches an initial peak (Guilbot etal. 1961).…”

Section: Water Vapor Sorption By Dry Starchmentioning

confidence: 99%

Textural Qualities and Molecular Structure of Starch Products

Sterling¹

1978

Journal of Texture Studies

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This review deals with the physical properties o f starch important in foods and with some o f the factors which influence those properties. The major topics discussed are the molecular structure of the starch grain, the interaction of starch with water and with other compounds, the rheology of starch and starch products, and textural problems of starch in some representative foods.Journal of Texture Studies 9 (1978) 225-255. All Rights Reserved @Copyright 1978 by Food

show abstract

Refractive indices of wheat starch granules at various moisture levels determined with an interference microscope

Cited by 18 publications

References 5 publications

Accelerated ex situ breeding of GBSS - and PTST1 -edited cassava for modified starch

Accelerated ex situ breeding of GBSS - and PTST1 -edited cassava for modified starch

Cell-to-Cell Diversity in a Synchronized Chlamydomonas Culture As Revealed by Single-Cell Analyses

Textural Qualities and Molecular Structure of Starch Products

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