Refractive index measurement of single living cells using on-chip Fabry-Pérot cavity

Song, Wuzhou; Zhang, Xuming; Liu, A. Q.; Lim, Chu Sing; Hosseini, Habib Mir M.

doi:10.1063/1.2387965

Cited by 140 publications

(94 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As one of the most popular commercial refractometers, Abbé refractometer could reach 10 −4 RIU by using the total internal reflection phenomenon. The wave optics based methods exploit the wave nature of light and mainly measure the RI by diffraction, 6 forward/backward scattering 7,8 and interference ͑e.g., Fabry-Pérot, [9][10][11] Mach-Zehnder, 12,13 double slits, 14 Michelson, 15 low coherence, 16 and fiber Bragg gratings 17,18 ͒. These methods improve the sensitivity to 10 −4 -10 −6 RIU since the wavelength acts naturally as the absolute reference of length.…”

Section: Introductionmentioning

confidence: 99%

Optofluidic refractometer using resonant optical tunneling effect

et al. 2010

Self Cite

View full text Add to dashboard Cite

This paper presents the design and analysis of a liquid refractive index sensor that utilizes a unique physical mechanism of resonant optical tunneling effect ͑ROTE͒. The sensor consists of two hemicylindrical prisms, two air gaps, and a microfluidic channel. All parts can be microfabricated using an optical resin NOA81. Theoretical study shows that this ROTE sensor has extremely sharp transmission peak and achieves a sensitivity of 760 nm/refractive index unit ͑RIU͒ and a detectivity of 85 000 RIU −1 . Although the sensitivity is smaller than that of a typical surface plasmon resonance ͑SPR͒ sensor ͑3200 nm/RIU͒ and is comparable to a 95% reflectivity Fabry-Pérot ͑FP͒ etalon ͑440 nm/RIU͒, the detectivity is 17 000 times larger than that of the SPR sensor and 85 times larger than that of the FP etalon. Such ROTE sensor could potentially achieve an ultrahigh sensitivity of 10 −9 RIU, two orders higher than the best results of current methods.

show abstract

Section: Introductionmentioning

confidence: 99%

Optofluidic refractometer using resonant optical tunneling effect

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…Moreover, it can enable direct label-free optical detection of subtle biochemical responses in the time range of milliseconds to nanoseconds, which would be useful for measurements of binding kinetics, molecular dynamics, secretion and drug responses of cells and many other processes. 4,[38][39][40][41] …”

Section: Discussionmentioning

confidence: 99%

“…Ultrafine 4 Comparison of the measured thermo-optic coefficients From the slopes and the error ranges of the curves in Figure 6 and using Equations (2) and (3), the thermo-optic coefficient (TOC) of ethanol is calculated to be dn/dT 5 (24.9 6 2.2) 3 10 24 6 C 21 by the OSA method and (24.328 6 0.009) 3 10 24 6 C 21 by the UFOFC method. The error is an intolerable 45% for the OSA method, but it is only 0.2% for the UFOFC method, which demonstrates that the UFOFC is more precise than the OSA method by a factor of 225.…”

Section: Sensing Arm N Lmentioning

confidence: 99%

A digitally generated ultrafine optical frequency comb for spectral measurements with 0.01-pm resolution and 0.7-µs response time

Bao

et al. 2015

Light Sci Appl

View full text Add to dashboard Cite

Optical spectral measurements are crucial for optical sensors and many other applications, but the prevailing methods, such as optical spectrum analysis and tunable laser spectroscopy, often have to make compromises among resolution, speed, and accuracy. Optical frequency combs are widely used for metrology of discrete atomic and molecular spectral lines. However, they are usually generated by optical methods and have large comb spacing, which limits the resolution for direct sampling of continuous spectra. To overcome these problems, this paper presents an original method to digitally generate an ultrafine optical frequency comb (UFOFC) as the frequency ruler for spectral measurements. Each comb line provides one sampling point, and the full spectrum can be captured at the same time using coherent detection. For an experimental demonstration, we adopted the inverse fast Fourier transform to generate a UFOFC with a comb spacing of 1.46 MHz over a 10-GHz range and demonstrated its functions using a Mach-Zehnder refractive index sensor. The UFOFC obtains a spectral resolution of 0.01 pm and response time of 0.7 ms; both represent 100-fold improvements over the state of the art and could be further enhanced by several orders of magnitude. The UFOFC presented here could facilitate new label-free sensor applications that require both high resolution and fast speed, such as measuring binding kinetics and single-molecule dynamics.

show abstract

“…32 Different from the normal on-chip interferometer that requires optical fibers and complicated package. 29,33 Recently, we induced the imaging based interferometer integrated on chip based on the mechanism of localized thin film interference. 34,35 In this work, we demonstrated a versatile imaging based method for simultaneously determining the microfluidic pressure and flow rate on a chip with the integrated optofluidic membrane interferometers (OMIs).…”

Section: Introductionmentioning

confidence: 99%

“…Lien and Vollmer reported a microfluidic flow sensor based on integrated optical fiber cantilever, but it also requires complicated fabrication and packaging process. 16 As an emerging field, optofluidics has enabled a number of novel optical functional devices built on microfluidic systems, [17][18][19][20][21][22][23] such as optofluidic lens, 24 light sources, [25][26][27] molecule sensors, 28 interferometers, 29,30 optofluidic prism, 31 and color filter. 32 Different from the normal on-chip interferometer that requires optical fibers and complicated package.…”

Section: Introductionmentioning

confidence: 99%

Optofluidic membrane interferometer: An imaging method for measuring microfluidic pressure and flow rate simultaneously on a chip

Song

2011

Biomicrofluidics

View full text Add to dashboard Cite

We present a novel image-based method to measure the on-chip microfluidic pressure and flow rate simultaneously by using the integrated optofluidic membrane interferometers (OMIs). The device was constructed with two layers of structured polydimethylsiloxane (PDMS) on a glass substrate by multilayer soft lithography. The OMI consists of a flexible air-gap optical cavity which upon illumination by monochromatic light generates interference patterns that depends on the pressure. These interference patterns were captured with a microscope and analyzed by computer based on a pattern recognition algorithm. Compared with the previous techniques for pressure sensing, this method offers several advantages including low cost, simple fabrication, large dynamic range, and high sensitivity. For pressure sensing, we demonstrate a dynamic range of 0-10 psi with an accuracy of 62% of full scale. Since multiple OMIs can be integrated into a single chip for detecting pressures at multiple locations simultaneously, we also demonstrated a microfluidic flow sensing by measuring the differential pressure along a channel. Thanks to the simple fabrication that is compatible with normal microfluidics, such OMIs can be easily integrated into other microfluidic systems for in situ fluid monitoring.

show abstract

Refractive index measurement of single living cells using on-chip Fabry-Pérot cavity

Cited by 140 publications

References 14 publications

Optofluidic refractometer using resonant optical tunneling effect

Optofluidic refractometer using resonant optical tunneling effect

A digitally generated ultrafine optical frequency comb for spectral measurements with 0.01-pm resolution and 0.7-µs response time

Optofluidic membrane interferometer: An imaging method for measuring microfluidic pressure and flow rate simultaneously on a chip

Contact Info

Product

Resources

About