Reformation of organized epidermal structure by transplantation of suspensions and cultures of epidermal and dermal cells

Worst, P.; Mackenzie, Ian C.; Fusenig, N. E.

doi:10.1007/bf00216219

Cited by 76 publications

(35 citation statements)

References 26 publications

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“…This results in the reconstitution of well-stratified epidermis (13,14). The grafting technique that we have adopted, developed by N. Fusenig and coworkers (13,14), involves the use of dome-shaped silicone transplantation chambers that are placed onto the back ofthe mouse in a way that prevents invasion of the graft bed by the surrounding host skin.…”

Section: Resultsmentioning

confidence: 99%

Malignant transformation of mouse primary keratinocytes by Harvey sarcoma virus and its modulation by surrounding normal cells.

Dotto

Weinberg

Ariza

1988

Proc. Natl. Acad. Sci. U.S.A.

107

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Section: Resultsmentioning

confidence: 99%

Malignant transformation of mouse primary keratinocytes by Harvey sarcoma virus and its modulation by surrounding normal cells.

Dotto

Weinberg

Ariza

1988

Proc. Natl. Acad. Sci. U.S.A.

107

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“…Several cellular suspensions with or without collagen were added to the chamber. It was shown that dissociated skin cells, containing both epidermal and connective tissue elements, re-form histologically normal epidermis and skin appendages (Worst et al, 1982).…”

Section: (Ai12) Allgenic and Allogenic Acellular Dermismentioning

confidence: 99%

Tissue Engineering of Skin

Pomahac¹,

Svensjö²,

Yao³

et al. 1998

Critical Reviews in Oral Biology & Medicine

102

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The skin plays a crucial role in protecting the integrity of the body's internal milieu. The loss of this largest organ is incompatible with sustained life. In reconstructive surgery or burn management, substitution of the skin is often necessary. In addition to traditional approaches such as split-or full-thickness skin grafts, tissue flaps and free-tissue transfers, skin bioengineering in vitro or in vivo has been developing over the past decades. It applies the principles and methods of both engineering and life sciences toward the development of substitutes to restore and maintain skin structure and function. Currently, these methods are valuable alternatives or complements to other techniques in reconstructive surgery. This review article deals with the evolution and current approaches to the development of in vitro and in vivo epidermis and dermis.

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“…It has been known for years that interactions between epithelial and mesenchymal tissues play a role in the differentiation and maintenance of the skin and oral epithelia Dale et al, 1990;Sengel, 1990), and further that a connective tissue component is essential for the growth and maturation of cultured (Briggaman et al, 1967;Worst et al, 1982), and grafted (Mackenzie and Hill, 1981;De Luca et al, 1990) epithelial cells. Cultured keratinocytes show full differentiation only if associated with a connective tissue or with fibroblasts (Rheinwald and Green, 1975;Mackenzie and Hill, 1984;Mackenzie and Dabelsteen, 1987).…”

Section: Discussionmentioning

confidence: 99%

Recapitulation of oral mucosal tissues in long‐term organotypic culture

et al. 2003

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To test the influence of fibroblasts on epithelial morphology and expression of keratinocyte proteins and barrier lipids, we bioengineered homotypic and heterotypic oral mucosae and skin using cultured adult human cells. Fibroblasts were allowed to modify collagen type I gels for 2 weeks before keratinocytes were added. The organotypic cultures were then grown at the air-liquid interface for 4 weeks. In homotypic combinations, epithelial morphology and protein expression closely mimicked those in vivo. In heterotypic combinations, the morphology resembled that in vivo and keratinocytes expressed their typical markers, except when skin keratinocytes were recombined with alveolar fibroblasts; they expressed K19, K4, and K13, which is similar to oral mucosal epithelia rather than to the epidermis. Morphologically, the stratum corneum layers were typical for the epithelial tissues. Grafting the bioengineered cultures to the backs of Nude mice did not change the results, suggesting that our findings are not merely a culture phenomenon. Lipid profiles of the homotypic combinations mimicked the profiles found in the normal epithelial tissues, except that the engineered alveolar epithelium expressed more ceramide 2 than that in vivo. In the heterotypic combinations, keratinocytes appeared to control the lipid profile, except in the combination of skin keratinocytes with alveolar fibroblasts, wherein the ceramide profile appeared to be partly that of alveolar epithelium and partly that of epidermis. These results suggest that cultured adult fibroblasts and keratinocytes are sufficient to recapitulate graftable oral tissues, and, except for alveolar fibroblasts, the type of fibroblast had little influence on keratinocyte differentiation. Anat Rec Part A 270A: 162-174, 2003.

show abstract

Reformation of organized epidermal structure by transplantation of suspensions and cultures of epidermal and dermal cells

Cited by 76 publications

References 26 publications

Malignant transformation of mouse primary keratinocytes by Harvey sarcoma virus and its modulation by surrounding normal cells.

Malignant transformation of mouse primary keratinocytes by Harvey sarcoma virus and its modulation by surrounding normal cells.

Tissue Engineering of Skin

Recapitulation of oral mucosal tissues in long‐term organotypic culture

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