Refolding of thermally denatured cholesterol oxidases by magnetic nanoparticles

Ghosh, Sudipta; Ahmad, Razi; Khare, Sunil Kumar

doi:10.1016/j.ijbiomac.2019.07.103

Cited by 20 publications

(7 citation statements)

References 41 publications

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“…The Vinogradov group reported alumina NPs as agents to promote renaturation of misfolded enzymes with overall anionic charge [37]. More recently, Khare demonstrated magnetic iron oxide nanoparticles featuring aminopropyl triethoxysilane (APTES) modifications that were capable of refolding thermally denatured enzymes [38]. After incubation between APTES-NPs and thermally denatured cholesterol oxidases, dynamic light scattering (DLS), zeta potential measurements, fluorescence and CD spectroscopy confirmed enzyme refolding to the native state.…”

Section: Regulation Of Enzyme Activitymentioning

confidence: 99%

Engineering the Interface between Inorganic Nanoparticles and Biological Systems through Ligand Design

et al. 2021

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Nanoparticles (NPs) provide multipurpose platforms for a wide range of biological applications. These applications are enabled through molecular design of surface coverages, modulating NP interactions with biosystems. In this review, we highlight approaches to functionalize nanoparticles with ”small” organic ligands (Mw < 1000), providing insight into how organic synthesis can be used to engineer NPs for nanobiology and nanomedicine.

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Section: Regulation Of Enzyme Activitymentioning

confidence: 99%

Engineering the Interface between Inorganic Nanoparticles and Biological Systems through Ligand Design

et al. 2021

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“…Nanoparticles have been widely used for decades due to their nanoscale size, mobility, multifunctionality, ability to adapt, increased solubility, personalized medications, early detection, and disease prevention ( Ahmad and Sardar, 2015 ; Ahmad and Khare, 2018 ; Ghosh et al, 2018a , b ; Soares et al, 2018 ). Nanomedicine has been used successfully to enhance treatment for a wide range of illnesses including neural, cancer, cardiopulmonary, and communicable diseases like HIV-1, HBV, influenza virus, and respiratory syncytial virus ( Ahmad et al, 2014b ; Ghosh et al, 2019 , 2021a , b ; Abd Ellah et al, 2020 ; Srivastava et al, 2021 ). Nanotechnology could play a potential role in diagnosis, treatment, and prevention of COVID-19 ( Bhavana et al, 2020 ; Campos et al, 2020 ; Noori et al, 2020 ).…”

Section: Potential Strategies To Treat Covid-19mentioning

confidence: 99%

COVID-19 and SARS-CoV-2 Variants: Current Challenges and Health Concern

et al. 2021

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The ongoing coronavirus disease 2019 (COVID-19) outbreak in Wuhan, China, was triggered and unfolded quickly throughout the globe by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The new virus, transmitted primarily through inhalation or contact with infected droplets, seems very contagious and pathogenic, with an incubation period varying from 2 to 14 days. The epidemic is an ongoing public health problem that challenges the present global health system. A worldwide social and economic stress has been observed. The transitional source of origin and its transport to humans is unknown, but speedy human transportation has been accepted extensively. The typical clinical symptoms of COVID-19 are almost like colds. With case fatality rates varying from 2 to 3 percent, a small number of patients may experience serious health problems or even die. To date, there is a limited number of antiviral agents or vaccines for the treatment of COVID-19. The occurrence and pathogenicity of COVID-19 infection are outlined and comparatively analyzed, given the outbreak’s urgency. The recent developments in diagnostics, treatment, and marketed vaccine are discussed to deal with this viral outbreak. Now the scientist is concerned about the appearance of several variants over the globe and the efficacy of the vaccine against these variants. There is a need for consistent monitoring of the virus epidemiology and surveillance of the ongoing variant and related disease severity.

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“…Disruption of the protein folding process due to external or internal stresses may result in protein misfolding. Owing to the ability of protein interaction, ENPs can severely affect the process of protein folding [ 71 , 72 ], aggregation [ 73 , 74 ] and fibrillation [ 75 , 76 ]. This characteristic of ENPs has been studied extensively due to the potential effect of ENPs on various essential proteins.…”

Section: Enps Interaction With Proteinsmentioning

confidence: 99%

Engineered Nanoparticle-Protein Interactions Influence Protein Structural Integrity and Biological Significance

et al. 2022

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Engineered nanoparticles (ENPs) are artificially synthesized particles with unique physicochemical properties. ENPs are being extensively used in several consumer items, elevating the probability of ENP exposure to biological systems. ENPs interact with various biomolecules like lipids, proteins, nucleic acids, where proteins are most susceptible. The ENP-protein interactions are mostly studied for corona formation and its effect on the bio-reactivity of ENPs, however, an in-depth understanding of subsequent interactive effects on proteins, such as alterations in their structure, conformation, free energy, and folding is still required. The present review focuses on ENP-protein interactions and the subsequent effects on protein structure and function followed by the therapeutic potential of ENPs for protein misfolding diseases.

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Refolding of thermally denatured cholesterol oxidases by magnetic nanoparticles

Cited by 20 publications

References 41 publications

Engineering the Interface between Inorganic Nanoparticles and Biological Systems through Ligand Design

Engineering the Interface between Inorganic Nanoparticles and Biological Systems through Ligand Design

COVID-19 and SARS-CoV-2 Variants: Current Challenges and Health Concern

Engineered Nanoparticle-Protein Interactions Influence Protein Structural Integrity and Biological Significance

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