Reflective walled sample compartment and optimization of data acquisition geometry towards sensitive fluorescence measurement in fiber-optic setup

Panigrahi, Suraj Kumar; Mishra, Ashok Kumar

doi:10.1016/j.measurement.2019.02.076

Cited by 4 publications

(1 citation statement)

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“…In front-face fluorescence measurement, both the excitation and emission radiation is least attenuated due to frontal detection of fluorescence emission. Researchers prefer front-face fluorescence measurement for optically dense and turbid samples, where sample concentration is very high. , Although the significant attenuation of excitation and emission radiation is avoided (i.e., IFE is avoided) in front-face fluorescence measurement, still the resolution of fluorescence emission is not so great due to scattering contaminations from the cuvette surface, edges and sample itself.…”

Section: Resultsmentioning

confidence: 99%

Derived Absorbance Spectral Parameter as a Tool for Sensitive Fluorescence Measurements of Optically Dense Systems

Panigrahi

Mishra

2019

J. Phys. Chem. A

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The present study proposes an approach to use the derived absorbance spectral parameter (D abs) as a tool for sensitive fluorescence measurement of optically dense multifluorophoric systems. Mishra and group have demonstrated the use of D abs in obtaining optimum offset wavelength for sensitive fluorescence measurement in synchronous fluorescence spectra (SFS) (Anal. Chim. Acta 2016, 940, 113–119; Anal. Chim. Acta 2008, 630 (1), 47–56). The D abs is obtained with the knowledge of UV–vis absorbance spectral data of the system under study and fluorescence observation filed (FOF) parameters of the instrument. D abs is proportional to the observed fluorescence intensity of the system. Excitation of an optically dense sample at D abs maximum leads to maximum fluorescence intensity obtainable for that system even in the presence of considerable inner filter effect (IFE). The earlier studies were applicable to SFS at right-angle fluorescence measurement geometry and for samples showing only primary IFE. In the present study, the work has been expanded to general fluorescence measurements, even when both primary and secondary IFE is present and for different fluorescence measurement geometries (right-angle, 45° and front-face). The procedure for obtaining D abs is simple and can be applied to other analyte-specific/unconventional fluorescence measurement strategies reported in the literature.

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Section: Resultsmentioning

confidence: 99%