Reflections on the use of controls in immunohistochemistry and proposal for application of a multitissue spring-roll control block

Chan, John K. C.; Wong, Carmen; Ku, Wang; Kwan, Man-Yee

doi:10.1053/adpa.2000.17892

Cited by 26 publications

(7 citation statements)

References 3 publications

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“…Immunohistochemistry, as with any assay, needs to have the appropriate controls, both positive and negative (Leong and Leong, 2006; Chan et al, 2000; Maxwell and McCluggage, 2000). This was difficult to achieve with the whole hair methodology as each hair follicle could only be stained once.…”

Section: Discussionmentioning

confidence: 99%

The Demonstration of Immunohistochemical Biomarkers in Methyl Methacrylate-Embedded Plucked Human Hair Follicles

Randall

Foster

2007

Toxicol Pathol

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Plucked human hair follicles have been proposed as a potential surrogate for tumour tissue for measuring the effect of drugs on pharmacodynamic biomarkers in drug intervention studies. We describe a new technique of embedding plucked hair follicles in the acrylic resin, methyl methacrylate, and the immunohistochemical demonstration of six potential biomarkers (Ki67, EGFR, phospho-p27, phospho-histone H3, phospho-MAPK and phospho-Rb) in de-plasticised sections. The advantages of this technique over those that have been used in support of clinical drug trials, such as skin and tumour biopsies, whole blood and whole hair samples is discussed.

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Section: Discussionmentioning

confidence: 99%

The Demonstration of Immunohistochemical Biomarkers in Methyl Methacrylate-Embedded Plucked Human Hair Follicles

Randall

Foster

2007

Toxicol Pathol

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“…7,8 These may be constructed as multiple samples of one tumour type that cover a wide range of antigen expression (e.g. The latter include multi-tissue sandwich or sausage control blocks, multi-tissue spring roll blocks, tissue microarrays and punch biopsy control blocks.…”

Section: Positive and Negative Controlsmentioning

confidence: 99%

Quality management in immunohistochemistry

Eisen

2008

Diagnostic Histopathology

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“…Staining intensity was scored as follows: 0 = no staining, 1 = weak staining, 2 = moderate staining, and 3 = strong staining. The expression of CD56 assessed by multiple organ sausage block, including the central nervous system, served as a positive control [4]. We studied liver specimens from patients with prenatally diagnosed CC, type-1 cystic BA, and the control group, and classified them immunohistologically and investigated retrospectively whether the pathological findings of CD56-immunostaining of these liver specimens could discriminate between prenatally diagnosed CC and type-1 cystic BA.…”

Section: Immunohistochemistry Of the Cd56-stained Liver Biopsy Specimensmentioning

confidence: 99%

Comparison between Prenatally Diagnosed Choledochal Cyst and Type-1 Cystic Biliary Atresia by CD56-Immunostaining Using Liver Biopsy Specimens

Okada¹,

Itoh²,

Sasaki³

et al. 2007

Eur J Pediatr Surg

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Because it is difficult to distinguish preoperatively between a prenatally diagnosed choledochal cyst (CC) and type-1 cystic biliary atresia (BA) by ultrasound scanning or magnetic resonance imaging, some mode of discriminating between the 2 entities is required. The aim of this study was to investigate the immunohistological differences between prenatally diagnosed CC and type-1 cystic BA, using liver biopsy specimens immunostained for CD56. Five children with prenatally diagnosed CC and two children with prenatally diagnosed type-1 cystic BA were identified by fetal ultrasonography between 1985 and 2004. The control group included two children who were operated on at an earlier period due to postnatally diagnosed BA. Liver wedge biopsy in the right lobe was performed at the time of the radical operation. Histological findings of the CD56-stained liver biopsy specimens were classified into 4 categories each, with particular focus on staining distribution and intensity. The staining distribution was classified according to the scale 0 = no staining; 1 = some staining of bile ducts/ductules but staining in less than one-third of portal tracts; 2 = staining in one-third to less than two-thirds of portal tracts; and 3 = staining in more than two-thirds of portal tracts. Staining intensity was scored as follows: 0 = no staining, 1 = weak staining, 2 = moderate staining, and 3 = strong staining. The staining intensity and distribution in the CC group was zero in all 5 cases. The type-1 cystic BA group consisted of patients with scale 1 or 3 staining distribution and score 1 or 2 staining intensity. In the control group, staining distribution was 1 or 3, and staining intensity was 1 or 3. These results indicate that CD56-positive biliary duct cells are present in prenatally diagnosed type-1 cystic BA. The authors suggest that exploratory laparotomy might be avoided and, instead, immunohistological examination using liver biopsy specimens may be a reliable test for the differential diagnosis of CC and type-1 cystic BA in prenatally diagnosed neonates.

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Reflections on the use of controls in immunohistochemistry and proposal for application of a multitissue spring-roll control block

Cited by 26 publications

References 3 publications

The Demonstration of Immunohistochemical Biomarkers in Methyl Methacrylate-Embedded Plucked Human Hair Follicles

The Demonstration of Immunohistochemical Biomarkers in Methyl Methacrylate-Embedded Plucked Human Hair Follicles

Quality management in immunohistochemistry

Comparison between Prenatally Diagnosed Choledochal Cyst and Type-1 Cystic Biliary Atresia by CD56-Immunostaining Using Liver Biopsy Specimens

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