Reflections on Ten Years of History of, and Future Prospects for, GW182 and GW/P Body Research

Chan, Edward K. L.; Yao, Bing; Fritzler, Marvin J.

doi:10.1007/978-1-4614-5107-5_15

Cited by 5 publications

(3 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ago-Abs are known to be involved in RNA metabolism, as several other antibodies identified in rheumatological disorders 5 10. Their presence has been recently reported in other autoimmune diseases, including neurological conditions, underlining their connection with a dysregulation of the immune system 6 7. In our cohort, almost all Ago-Abs-positive patients presented AQP4-NMOSD, whose association with other systemic autoimmune diseases is well established 3.…”

Section: Discussionsupporting

confidence: 54%

“…Argonaute proteins are part of RNA-binding proteins involved in the RNA silencing pathway 5. Anti-Argonaute antibodies (Ago-Abs) were first described in patients with rheumatological conditions, including systemic lupus erythematosus, scleroderma, polymyositis/dermatomyositis and Sjögren’s syndrome, displaying a strong link with systemic autoimmunity 6. Recently, Ago-Abs were reported as a potential new biomarker in patients with neurological disorders including sensory neuronopathies, limbic encephalitis and, less frequently, cerebellar syndrome, opsoclonus–myoclonus and length-dependent polyneuropathies.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-Argonaute antibodies as a potential biomarker in NMOSD

Carta

Rogemond

et al. 2023

J Neurol Neurosurg Psychiatry

View full text Add to dashboard Cite

Background and objectivesNeuromyelitis optica spectrum disorders (NMOSDs) are a group of diseases mainly characterised by recurrent optic neuritis and/or myelitis. Most cases are associated with a pathogenic antibody against aquaporin-4 (AQP4-Ab), while some patients display autoantibodies targeting the myelin oligodendrocyte glycoprotein (myelin oligodendrocyte glycoprotein antibodies (MOG-Abs)). Anti-Argonaute antibodies (Ago-Abs) were first described in patients with rheumatological conditions and were recently reported as a potential biomarker in patients with neurological disorders. The aims of the study were to investigate if Ago-Abs can be detected in NMOSD and to evaluate its clinical usefulness.MethodsSera from patients prospectively referred to our centre with suspected NMOSD were tested for AQP4-Abs, MOG-Abs and Ago-Abs with cell-based assays.ResultsThe cohort included 104 prospective patients: 43 AQP4-Abs-positive cases, 34 MOG-Abs positive cases and 27 double-negative patients. Ago-Abs were detected in 7 of 104 patients (6.7%). Clinical data were available for six of seven patients. The median age at onset of patients with Ago-Abs was 37.5 [IQR 28.8–50.8]; five of six patients tested positive also for AQP4-Abs. Clinical presentation at onset was transverse myelitis in five patients, while one presented with diencephalic syndrome and experienced a transverse myelitis during follow-up. One case presented a concomitant polyradiculopathy. Median EDSS score at onset was 7.5 [IQR 4.8–8.4]; median follow-up was 40.3 months [IQR 8.3–64.7], and median EDSS score at last evaluation was 4.25 [IQR 1.9–5.5].ConclusionAgo-Abs are present in a subset of patients with NMOSD and, in some cases, represent the only biomarker of an autoimmune process. Their presence is associated with a myelitis phenotype and a severe disease course.

show abstract

Section: Discussionsupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Anti-Argonaute antibodies as a potential biomarker in NMOSD

Carta

Rogemond

et al. 2023

J Neurol Neurosurg Psychiatry

View full text Add to dashboard Cite

show abstract

“…Replication of DENV RNA in vivo is associated with P-body. P-bodies are distinct foci within the cytoplasm of the eukaryotic cell and play fundamental roles in mRNA translation, RNA silencing, and RNA degradation as well as viral infection processes (20)(21)(22). In the present study, we examined whether the positive regulating role of LSm1 in DENV RNA replication was associated with P-body.…”

Section: Lsm1 Depletion Inhibits the Replication Of Denv Rna And Relementioning

confidence: 96%

LSm1 binds to the Dengue virus RNA 3′ UTR and is a positive regulator of Dengue virus replication

Dong

Yang

et al. 2015

International Journal of Molecular Medicine

View full text Add to dashboard Cite

Dengue virus (DENV) is a mosquito-transmitted flavivirus that can cause severe disease in humans. The DENV positive strand RNA genome contains 5' and 3' untranslated regions (UTRs) that have been shown to be required for virus replication and interaction with host cell proteins. In the present study LSm1 was identified as a host cellular protein involved in DENV RNA replication. By using two independent methodologies, we demonstrated a critical interaction between LSm1 and the 3' UTR of DENV. Furthermore, the confocal immunofluorescence analysis showed that the interaction between LSm1 and viral RNA is located in P-body around nucleoli in the cytoplasm. LSm1 knockdown by siRNA specifically reduced the levels of viral RNA in DENV-infected cells and infectious DENV particles in the supernatant. These results provide evidence that LSm1 binding to the DENV RNA 3' UTR positively regulates DENV RNA replication.

show abstract

The association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formation

et al. 2023

View full text Add to dashboard Cite

Stress granules (SGs) are dynamic, non-membranous structures composed of non-translating mRNAs and various proteins and play critical roles in cell survival under stressed conditions. Extensive proteomics analyses have been performed to identify proteins in SGs; however, the molecular functions of these components in SG formation remain unclear. In this report, we show that ubiquitin-associated protein 2-like (UBAP2L) is a crucial component of SGs. UBAP2L localized to SGs in response to various stresses, and its depletion significantly suppressed SG organization. Proteomics and RNA sequencing analyses found that UBAP2L formed a protein-RNA complex with Ras-GTP-activating protein SH3 domain binding protein 1 (G3BP1) and small nucleolar RNAs (snoRNAs). In vitro binding analysis demonstrated that snoRNAs were required for UBAP2L association with G3BP1. In addition, decreased expression of snoRNAs reduced the interaction between UBAP2L and G3BP1 and suppressed SG formation. Our results reveal a critical role of SG component, the UBAP2L/snoRNA/G3BP1 protein-RNA complex, and provide new insights into the regulation of SG assembly.

show abstract

Reflections on Ten Years of History of, and Future Prospects for, GW182 and GW/P Body Research

Cited by 5 publications

References 50 publications

Anti-Argonaute antibodies as a potential biomarker in NMOSD

Anti-Argonaute antibodies as a potential biomarker in NMOSD

LSm1 binds to the Dengue virus RNA 3′ UTR and is a positive regulator of Dengue virus replication

The association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formation

Contact Info

Product

Resources

About