Refining the application of direct embryogenesis in sugarcane: effect of the developmental phase of leaf disc explants and the timing of DNA transfer on transformation efficiency

Snyman, S. J.; Meyer, G. M.; Richards, J. M.; Haricharan, N.; Ramgareeb, S.; Huckett, B. I.

doi:10.1007/s00299-006-0148-z

Cited by 69 publications

(64 citation statements)

References 39 publications

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“…For this study, 4 sugarcane varieties were selected, namely cultivars CPF-234, CPF-213, HSF-240, and CPF-246, and their young immature leaves were used as explants to induce calli. Snyman et al (2006) also suggested that immature leaves of sugarcane prove to be an excellent explant for production of an embryogenic, friable callus. Optimization for friable, embryogenic calli is not only mandatory for genetic manipulation of sugarcane but also strengthens the development of the transformation system in sugarcane (Fitch et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Herbicide-tolerant sugarcane (Saccharum officinarum L.) plants: an unconventional method of weed removal

Nasir¹,

Tabassum²,

Qamar³

et al. 2014

Turk J Biol

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Unnecessary weed growth in sugarcane fields forces plants to compete for nutrients and sunlight for survival, which most often leads to significant yield losses. As chemical herbicides cannot differentiate between crop plants and weeds, the development of herbicide-tolerant crops is anticipated. Four sugarcane varieties, CPF-234, CPF-213, HSF-240, and CPF-246, were used to develop glyphosate herbicide tolerance. A glyphosate-tolerant gene of 1368 bp cloned directionally under the 35S promoter with the β-glucuronidase (GUS) reporter gene was used as the transgene. Through the biolistic transformation of sugarcane, calli of all cultivars were transformed with glyphosate-tolerant gene constructs. Efficient regeneration conditions were optimized on 1.5-2.5 mg/L kinetin, 1.5-2.5 mg/L 6-benzylaminopurine (BAP), and 1 mg/L gibberellic acid (GA 3 ). The transformants exhibited the best shoot regeneration on a medium containing 2 mg/L kinetin, 2 mg/L BAP, 2 mg/L GA 3 , and 1 mg/L indole-3-acetic acid. Based on initial screenings through GUS assay, the transformation efficiency was 22%, 32%, 17%, and 13% for cultivars 246, 234, 213, and 240, respectively. In transformed sugarcane plants, the transgene of 1368 bp was amplified. ELISA with gene-specific coated monoclonal IgG confirmed the transgene protein expression. It was revealed that all acclimatized transgenic sugarcane plants survived the glyphosate spray application of 900 mL/0.404 ha, except for the control nontransformed plants. However, at spray application of 1100 mL/0.404 ha, transgenic plants having the transgene protein OD of 0.2 to 1.0 did not survive, while those that had a transgene protein OD range of between 1.0 and 2.0 did. In addition, weeds growing alongside transgenic sugarcane plants turned brown and subsequently died at glyphosate spray applications of both 900 and 1100 mL/0.404 ha.

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Section: Discussionmentioning

confidence: 99%

Herbicide-tolerant sugarcane (Saccharum officinarum L.) plants: an unconventional method of weed removal

Nasir¹,

Tabassum²,

Qamar³

et al. 2014

Turk J Biol

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“…Sob ausência de auxina no meio de regeneração, os brotos regenerados provenientes de embriões somáticos foram produzidos neste experimento, assim como os trabalhos de Falco et al (2000) e Snyman et al (2006). Visando obter plantas bem desenvolvidas, os brotos regenerados foram subcultivados novamente em meio de regeneração por 21 dias.…”

Section: Methodsunclassified

Indução de embriogênese somática em cana-de-açúcar RB935744

Almeida

Silva

Vargas

et al. 2014

AGGA

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O objetivo, com este trabalho, foi avaliar a resposta morfogênica do genótipo RB935744 à indução de embriogênese somática com subsequente regeneração dos embriões. Segmentos transversais de folhas jovens medindo 2-3 mm de espessura foram cultivados em meio MS sólido contendo três diferentes concentrações de ácido 2,4-diclorofenoxiacético (2,4-D) para indução de calos embriogênicos. Os -1 melhores resultados foram obtidos usando 31,5 µM L de 2,4-D em 45 ou 60 dias de incubação em meio de indução, realizando de um a três subcultivos e incubando em meio de regeneração por período de 15 a 45 dias. Esse resultado mostra que o protocolo de embriogênese somática para o genótipo RB935744 necessita de ajustes para se alcançar uma satisfatória produção de embriões somáticos.Palavras-chave: Saccharum spp. Cultura de tecido vegetal. Regeneração in vitro.

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“…It has now become a valuable alternative to the conventional clonal propagation methods for seed production. Tissue culture can increase the propagation potential by 20-35 times (Geijskes et al, 2003, Snyman et al, 2006. In addition, plants can be disease-indexed (Snyman et al, 2007) and healthy material multiplied in much less time compared to the conventional vegetative route.…”

Section: Introductionmentioning

confidence: 99%

Comparative performance of micropropagated and conventional plants of sugarcane

2016

Int. J. Adv. Res. Biol. Sci.

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An experiment was performed to study the comparative performance of micropropagated and conventional plants. A total of 200 micropropagated plantlets of each variety, hardened for one month, were transplanted in the field. Simultaneously, the donor varieties were also planted using 1 bud-setts in three rows for comparison of various qualitative traits like Brix %, Sucrose % and Purity %. A comparative perusal of data showed that the sugarcane variety CoSe 01235 had higher Brix % as well as Sucrose % than the variety CoS 99259 in their respective counterparts, in a particular month. The Brix % and Sucrose % were marginally higher in tissue cultured plants than their donors in all the three months. However, the purity % was recorded to be marginally higher in CoS 99259 in both micropropagated and donor plants as compared to the respective plants of variety CoSe 01235.

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Refining the application of direct embryogenesis in sugarcane: effect of the developmental phase of leaf disc explants and the timing of DNA transfer on transformation efficiency

Cited by 69 publications

References 39 publications

Herbicide-tolerant sugarcane (Saccharum officinarum L.) plants: an unconventional method of weed removal

Herbicide-tolerant sugarcane (Saccharum officinarum L.) plants: an unconventional method of weed removal

Indução de embriogênese somática em cana-de-açúcar RB935744

Comparative performance of micropropagated and conventional plants of sugarcane

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