Refining insulin concentrations in culture medium containing growth factors BMP15 and GDF9: An in vitro study of the effects on follicle development of goats

Dipaz-Berrocal, D.; Sá, Naíza Arcângela Ribeiro de; Guerreiro, Denise Damasceno; Celestino, J.J.H.; Leiva-Revilla, Johanna; Alves, Bênner Geraldo; Alves, Kele Amaral; Santos, Regiane R.; Cibin, Francielli Weber Santos; Rodrigues, Ana Paula Ribeiro; Figueiredo, J.R.

doi:10.1016/j.anireprosci.2017.08.011

Cited by 11 publications

(6 citation statements)

References 28 publications

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“…On the other hand, GDF-9 increased the daily growth rate from the first to the second third of culture, with higher values (P < 0.05) in the second third compared to control (Table 3). However, this difference dissipated in the last third of culture (days [12][13][14][15][16][17][18]. Unlike ours, a previous study showed that GDF-9 promoted the in vitro growth of human preantral follicles enclosed in ovarian tissue by stimulating the proliferation and differentiation of granulosa cells (5) .…”

Section: Resultsmentioning

confidence: 68%

“…In a recent study, the addition of GDF-9 at the same concentration as that used in this study also did not affect the viability of preantral follicles included in cultured ovarian fragments (15) . Moreover, supplementation of GDF-9 during in vitro culture of goat preantral follicles plus physiological concentrations of insulin (10 ng/mL) equally contained in the medium seems to have been unnecessary for follicle development (16) . The result obtained in this study is similar to those of previous works in which the addition of insulin (10 ng/mL) alone proved capable of maintaining the viability of isolated antral follicles (17,18) .…”

Section: Resultsmentioning

confidence: 99%

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Growth and differentiation factor - 9 (GDF-9) increases the in vitro growth rates of isolated goat early antral follicles

Santos,

Ferreira,

Sá

et al. 2023

Ciênc. anim. bras.

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This study aimed to investigate the effect of growth and differentiation factor 9 (GDF-9) during the in vitro culture of isolated caprine early antral follicles. The isolated and selected early antral follicles were individually cultured for 18 days, and the following treatments were tested: α-MEM+ (control treatment) or α-MEM+ supplemented with 200 ng/mL GDF-9. The following endpoints were evaluated: follicular growth and morphology, estradiol production, oocyte nuclear maturation, and relative expression of key genes related to steroidogenesis (CYP19A1, CYP17, and insulin receptor) and basement membrane remodeling (MMP-9 and TIMP-2). In both treatments, a decrease was observed in the percentage of morphologically intact follicles with a concomitant increase in the rates of extruded and degenerated follicles (P < 0.05). The GDF-9 treatment showed higher rates of extruded follicles only on day 6 of culture (P < 0.05). Follicle diameter increased progressively throughout the culture period (P < 0.05) with similar diameters between treatments at all culture times (P > 0.05). Growth and differentiation factor 9 increased the daily growth rate from the first to the second third of culture, with higher values (P < 0.05) than control in the second third. Oocyte maturation rate as well as estradiol levels and relative mRNA expression for CYP19A1, CYP17, MMP-9, TIMP-2, and insulin receptor genes were similar between treatments (P > 0.05). This study shows for the first time that GDF-9 added to a culture medium increased the follicle growth rate of goat early antral follicles cultured in vitro.

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Section: Resultsmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 99%

Growth and differentiation factor - 9 (GDF-9) increases the in vitro growth rates of isolated goat early antral follicles

Santos,

Ferreira,

Sá

et al. 2023

Ciênc. anim. bras.

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“…Num estudo recente, a adição de GDF-9 na mesma concentração que a utilizada neste estudo também não afetou a viabilidade de folículos pré-antrais incluídos em fragmentos ovarianos de catetos (15) . Além disso, a suplementação de GDF-9 durante o cultivo in vitro de folículos pré-antrais caprinos acrescida de concentrações fisiológicas de insulina (10 ng/mL) igualmente contidas no meio, parece ter sido desnecessária para o desenvolvimento folicular (16) . O resultado obtido neste estudo é semelhante aos resultados de trabalhos anteriores em que a adição de insulina (10 ng/mL), por si só, mostrou-se capaz de manter a viabilidade de folículos antrais isolados (17,18) .…”

Section: Resultsunclassified

Fator de crescimento e diferenciação - 9 (GDF-9) aumenta a taxa de crescimento in vitro de folículos antrais iniciais caprinos isolados

Santos,

Ferreira,

Sá

et al. 2023

Ciênc. anim. bras.

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Resumo Este estudo teve como objetivo investigar o efeito do GDF-9 durante o cultivo in vitro de folículos antrais iniciais caprinos isolados. Os folículos antrais iniciais isolados e selecionados foram cultivados individualmente por 18 dias, e os seguintes tratamentos foram testados: α MEM+ (tratamento controle) ou α-MEM+ suplementado com 200 ng/mL de GDF-9 (tratamento GDF-9). Os seguintes parâmetros foram avaliados: crescimento e morfologia folicular, produção de estradiol, maturação nuclear do oócito e expressão relativa de genes-chave relacionados a esteroidogênese (CYP19A1, CYP17 e receptor de insulina) e remodelamento da membrana basal (MMP-9 e TIMP-2). Em ambos os tratamentos, observou-se diminuição na porcentagem de folículos morfologicamente intactos com aumento concomitante nas taxas de folículos extrusos e degenerados (P < 0,05). O tratamento GDF-9 apresentou maiores taxas de folículos extrusos apenas no 6º dia de cultivo (P < 0,05). O diâmetro do folículo aumentou progressivamente ao longo do período de cultivo (P < 0,05) com diâmetros semelhantes entre os tratamentos em todos os tempos de cultivo (P > 0,05). O GDF-9 aumentou a taxa de crescimento diário do primeiro para o segundo terço de cultivo, sendo maior (P < 0,05) que o controle no segundo terço. A taxa de maturação oocitária assim como os níveis de estradiol e a expressão relativa de RNAm para os genes CYP19A1, CYP17, MMP-9, TIMP-2 e receptor de insulina foram similares entre os tratamentos (P > 0,05). Em conclusão, este estudo mostra pela primeira vez que GDF-9 adicionado a um meio de cultivo aumentou a taxa de crescimento de folículos antrais iniciais caprinos cultivados in vitro.

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“…Insulin and FSH are present in almost every culture medium for IVFC, although their concentration when combined is still focus of discussion (Dipaz-Berrocal et al, 2017;Paes et al, 2018). It has been suggested that 10 ng/ml insulin, a lower concentration than that which comes in the ITS composition, could be more efficient in promoting meiotic resumption in the presence sequential FSH (Chaves et al, 2012).…”

Section: Caprinementioning

confidence: 99%

Advances in in vitro folliculogenesis in domestic ruminants

Figueiredo¹,

Lima²,

Santos³

2019

Anim Reprod

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The in vitro follicle culture (IVFC) represents an outstanding tool to enhance our understanding of the control of folliculogenesis and to allow the future use of a large number of immature oocytes enclosed in preantral follicles (PFs) in assisted reproductive techniques in humans as well as in others mammalian species including the ruminants. So far, the best results of IVFC were reported from mice with the production of live offspring from primordial follicles cultured in vitro. Live birth has been obtained after the in vitro culture of bovine early antral follicles. However, in other ruminant species, these results have been limited to the production of a variable number of mature oocytes and low percentages of embryos after in vitro culture of goat, buffalo and sheep isolated secondary preantral follicles. The present review presents and discusses the main findings, limitations, and prospects of in vitro folliculogenesis in ruminants focusing on bovine, caprine, and ovine species.

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Refining insulin concentrations in culture medium containing growth factors BMP15 and GDF9: An in vitro study of the effects on follicle development of goats

Cited by 11 publications

References 28 publications

Growth and differentiation factor - 9 (GDF-9) increases the in vitro growth rates of isolated goat early antral follicles

Growth and differentiation factor - 9 (GDF-9) increases the in vitro growth rates of isolated goat early antral follicles

Fator de crescimento e diferenciação - 9 (GDF-9) aumenta a taxa de crescimento in vitro de folículos antrais iniciais caprinos isolados

Advances in in vitro folliculogenesis in domestic ruminants

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