Refined technique for intestinal transplantation in the rat

Zhong, Robert; Grant, David; Sutherland, Francis; Wang, Pengzhi; Chen, Huifang; Lo, Steven; Stiller, C. R.; Duff, John

doi:10.1002/micr.1920120408

Cited by 68 publications

(54 citation statements)

References 23 publications

(8 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The success rate found in this study was 83.3%, which is comparable to those from literature. are also within the range reported in literature (15 to 25min 7,12 ; 45 to 67min 6,10,11 , 25min 9 and 113 to 120min 9,11 , respectively). Mean donor operation time in this study (40min) is shorter than found in others studies (57 12 , 81 11 and 89min 9 ).…”

Section: Clinical Indication Of Intestinal Transplantation Hassupporting

confidence: 90%

Small bowel transplantation in outbred rats

Waisberg¹,

Lee²,

Santos³

et al. 2011

Acta Cir. Bras.

View full text Add to dashboard Cite

PURPOSE:To investigate the clinical evolution of orthotopic small bowel transplantation in outbred rats. METHODS:Seventy-two outbred Wistar rats weighting from 250 to 300g were used as donor and recipient in 36 consecutives ortothopic small intestine transplantation without immunosuppression. The graft was transplanted into the recipient using end-to-side aortic and portacaval microvascular anastomosis. Procedure duration, animal clinical course and survival were evaluated. Survival shorter than four days was considered technical failure. Recipients were sacrificed with signs of severe graft rejection or survival longer than 120 days. Necropsies were performed in all recipients to access histopathological changes in the graft. RESULTS: Median time for the procedure was 107 minutes. Six recipients (16.7%) presented technical failure. Twenty-seven recipients were sacrificed due to rejection, being nineteen (52.7%) between 7 th and 15 th postoperative day and eight (22.2%) between 34 th and 47 th postoperative day. Graft histology confirmed severe acute cellular rejection in those recipients. Uneventful evolution and survival longer than 120 days without rejection were observed in three recipients (8.3%). CONCLUSION:Intestinal transplantation in outbred rats without immunosuppressant regiment accomplishes variable clinical evolution. Keywords

show abstract

Section: Clinical Indication Of Intestinal Transplantation Hassupporting

confidence: 90%

Small bowel transplantation in outbred rats

Waisberg¹,

Lee²,

Santos³

et al. 2011

Acta Cir. Bras.

View full text Add to dashboard Cite

show abstract

“…21 The intestinal transplantation techniques with the performance of vascular anastomoses are highly described in the literature. The method that supports our work was mostly the one used by Zong et al 7 Again, there were difficulties in applying the method, leading us to research for solutions and the introduction of some changes: The modification of some surgical technique steps was advantageous. Slight left median incision laparotomy, instead of a median one used by Zong et al, 7 made the access to the vein porta region easier.…”

Section: Discussionmentioning

confidence: 96%

“…20 However, most of the investigators prefer to revascularize the graft by aorta-aorta and porto-cava anastomoses. 1,7 The invoked reasons are technical simplicity, lack of significant changes on the hepatic metabolism, and high survival of the graft. 21 The intestinal transplantation techniques with the performance of vascular anastomoses are highly described in the literature.…”

Section: Discussionmentioning

confidence: 99%

Techniques of intestinal transplantation in rat

et al. 1998

View full text Add to dashboard Cite

Two surgical models of intestinal transplantation in the rat are described. One is the implantation of fetal and newborn intestine as free grafts into the omentum of adult recipients, the other the adult intestine transplantation as an accessory graft using vascular anastomoses. A hundred and sixteen smallbowel transplantations were done; 36 of which were fetal intestine (group I), 40 of newborn intestine (group II), and 40 of adult intestine (group III). In the fetal and newborn intestinal transplantation, we emphasize the practices that allowed us to avoid ischemic and traumatic injury to the graft. In the adult intestine transplantation with vascular anastomoses, we heighten the modifications in the surgical technique that made the operation easier and the strategies used to prevent hypothermia and hypovolemic shock. Once experienced with the two chosen surgical techniques, transplantation using an avascular segment became much easier and quicker than transplantation with vascular anastomoses.Os autores descrevem dois modelos cirú rgicos de transplantaçã o intestinal no rato. Um corresponde à implantação de intestino de feto e de intestino de recé m-nascido, como enxertos livres, no epíploon de receptores adultos, e o outro corresponde à transplantaçã o de intestino de adulto, como enxerto acessório, usando anastomoses vasculares. Fizémos 116 transplantaçõ es intestinais: 36 de intestino fetal (grupo I), 40 de intestino de recé m-nascido (grupo II) e 40 de intestino de adulto (grupo III). Realçá mos os mé todos utilizados para evitar a isqué mia e a lesã o traumá tica do enxerto, nas transplantaçõ es de intestino de feto e de recé m-nascido. Na transplantaçã o de intestino de adulto com anastomoses vasculares, enfatizá mos as modificações na té cnica cirú rgica, que facilitaram a operaçã o, e as estraté gias usadas para evitar a hipotermia e o choque hipovolé mico. Apó s a familiarizaçã o com os dois modelos cirú rgicos, a transplantação intestinal como enxerto avascular revelou-se de execução mais simples e mais rá pida do que a transplantaçã o intestinal com realizaçã o de anastomoses vasculares.© 1998 Wiley-Liss, Inc. MICROSURGERY 18:424-429 1998Nowadays, the laboratory rat (Rattus norvegicus) is one of the most popular models for intestinal transplantation. Its use started with Monchick and Russell's work 1,2 in the 1970s, in which they applied the surgical techniques with vascular anastomoses, previously developed in bigger models by other investigators. 3,4 The intestinal transplantation technique varies according to the kind of graft and the investigation. While studying immunogenicity of fetal, newborn, and adult jejunal allografts in the rat, the authors used two methods of small-bowel transplantation. Fetal and newborn grafts were implanted as an avascular segment into the omentum of adult recipients. Adult allograft was transplanted to an adult recipient as a heterotopic accessory graft using aorta-aorta and porto-caval anastomoses. 5 The aim of this study is to review the intestinal transplantatio...

show abstract

“…20 Over time, modifications have been made to this model to shorten procedure time and increase survival rate. [21][22][23][24][25] However, intestinal transplant has had a slower development versus other organs because of its complexity and severe immune response. 26 Heart transplant models can be established by several surgical methods, such as the abdominal double vascular anastomosis model of Ono and Lindsey, 27 the abdominal single artery anastomosis model of Lee and associates, 28 and the double cuff vascular anastomosis model of Heron.…”

Section: Validation Of Donor-specific Tolerance Of Intestinal Transplmentioning

confidence: 99%

Untitled

2017

Exp Clin Transplant

View full text Add to dashboard Cite

Objectives: It is well accepted that survival after a second organ transplant without immunosuppressive agents indicates tolerance for the first transplant. To validate donor-specific tolerance, we established a rat model with a secondary heart transplant after intestinal transplant, which has so far not been described in the literature. Materials and Methods: We transplanted intestine from Fischer F344 rats to Lewis rats orthotopically. Lewis rats received tacrolimus pretreatment before transplant and a 14-day course of rapamycin 1 month after transplant. At 120 days after primary intestinal transplant, hearts from 6 F344 rats (group A) or 6 Brown Norway rats (group B) were transplanted to Lewis rats that had survived intestinal transplant and without additional immunosuppressive agents. We analyzed survival data, histologic changes, cells positive for the ED1 macrophage marker in transplanted hearts, and 3 lymphocyte levels in both groups. Results: Thirty days after secondary heart transplant, group A hearts were continuously beating; however, group B hearts stopped beating at around 10 days after transplant (8.5 ± 1.5 d; P < .05). Our histologic study showed that both groups had muscle damage and cellular infiltration in hearts that were distinctly different from normal hearts, with ED1-positive cells counted in both groups (85 ± 16 in group A, 116 ± 28 in group B; P > .05). Fluorescence-activated cell sorting showed that CD4/CD25-positive regulatory T cell, CTLA4/CD4/CD25-positive regulatory T cell, and Natural killer T-cell levels were significantly higher level in group A versus B (P < .05). Conclusions:The donor-specific tolerance that we observed was possibly a state of "clinical tolerance" rather than "immunologic tolerance." Our rat model is a feasible and reliable model to study donor-specific tolerance. The higher levels of lymphocytic T cells shown in intestinal transplant recipients were associated with longer allograft survival, possibly contributing to donor-specific tolerance.

show abstract

Refined technique for intestinal transplantation in the rat

Cited by 68 publications

References 23 publications

Small bowel transplantation in outbred rats

Small bowel transplantation in outbred rats

Techniques of intestinal transplantation in rat

Untitled

Contact Info

Product

Resources

About