Here we report two cases of first-trimester parvovirus B19
CASE REPORTS Case 1A 32-year-old primigravida was referred to our unit after routine ultrasound examination revealed increased fetal nuchal translucency (NT) at 13 + 1 weeks' gestation. A detailed sonographic evaluation demonstrated an NT of 4.4 mm, biventricular myocardial hypertrophy, moderate skin edema, mild ascites and bilateral pleural effusion. The ductus venosus (DV) blood flow was normal (positive awave, pulsatility index for veins (PIV): 1.56) 1 . However, the measurement of the fetal middle cerebral artery peak systolic velocity (MCA-PSV) showed an increased velocity of 37 cm/s, suggestive of fetal anemia (Figure 1). Maternal parvovirus (PV) infection was diagnosed by determining PV-specific immunoglobulin-G (IgG) and IgM as well as PV-B19 DNA by polymerase chain reaction (PCR). Fetal karyotype was normal. Subsequently, fetal therapy by cordocentesis was offered. The placenta was located laterally. Because of the softness of the 25-G spinal needle and an inability to correct the intrauterine position, three attempts were needed to place the needle next to the umbilical cord insertion site. No paralyzing agent was used. The umbilical vein was then punctured in one attempt and 3 mL of packed red cells were transfused. The initial hemoglobin count was 0.8 g/dL. No bradycardia was observed during the procedure, which took about 2 minutes.After 2 days, decline of the MCA-PSV and complete resolution of the fetal hydrops were observed. Ultrasound and Doppler examinations were continued for a period of 10 weeks at 3-week intervals. Measurements of MCA-PSV continued to show velocities in the upper percentile range, but no additional signs of fetal anemia were detected. However, at 24 + 5 weeks' gestation the fetus presented again with severe hydrops fetalis and cardiomegaly. The measurement of MCA-PSV showed an increased velocity of 85 cm/s. Cordocentesis and intrauterine blood transfusion were performed. The initial hemoglobin count was 1.4 g/dL (reticulocytes 13.2%, thrombocytes 22/nL). PCR for PV-B19 DNA was positive in maternal blood as well as in fetal ascites and blood. Two further intrauterine blood transfusions followed (at 25 + 3 and 26 + 2 weeks' gestation) (Table 1) and finally