Reference Ranges for Lymphocyte Counts of Neonates: Associations Between Abnormal Counts and Outcomes

Christensen, Robert D.; Baer, Vickie L.; Gordon, P V; Henry, Erick; Whitaker, Cody; Andres, Robert L.; Bennett, Sterling T.

doi:10.1542/peds.2011-2661

Cited by 37 publications

(26 citation statements)

References 24 publications

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“…The publications of Christensen et al [20][21][22] on hematological reference values are a good functional fit with our study. The purpose of our study was to determine both developmental changes in hematopoiesis in the fetus after the 30th week of pregnancy and establish hematological reference values for cord blood based on gestational age, gender and the mode of delivery.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

A comprehensive study of umbilical cord blood cell developmental changes and reference ranges by gestation, gender and mode of delivery

Glasser¹,

Sutton²,

Schmeling³

et al. 2015

J Perinatol

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Section: Discussionsupporting

confidence: 89%

“…Lymphopenia with a high NRBC has been correlated with birth asphyxia and early onset sepsis while both increased and decreased LYMP counts are associated with intra-ventricular hemorrhage. 21 Virus infections may show lymphocytosis. In addition to chronic intrauterine hypoxia, increased NRBC may signify a hemolytic process.…”

Section: Discussionmentioning

confidence: 99%

A comprehensive study of umbilical cord blood cell developmental changes and reference ranges by gestation, gender and mode of delivery

Glasser¹,

Sutton²,

Schmeling³

et al. 2015

J Perinatol

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show abstract

“…The PLT counts increased for GAs of 22 to 42 weeks using a huge data system [8]. In contrast, an abnormal lymphocyte count at birth is associated with adverse outcomes, including early-onset sepsis, intraventricular hemorrhaging and retinopathy of prematurity [24]. The onset of neutropenia in the first days of life is sometimes noted in SGA infants or those born to mothers with persistent maternal hypertension or early-onset bacterial infection [25].…”

Section: Discussionmentioning

confidence: 99%

Blood Reference Intervals for Preterm Low-Birth-Weight Infants: A Multicenter Cohort Study in Japan

et al. 2016

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Preterm low-birth-weight infants remain difficult to manage based on adequate laboratory tests. The aim of this study was to establish blood reference intervals (RIs) in those newborns who were admitted to and survived in the neonatal intensive care unit (NICU). A multicenter prospective study was conducted among all infants admitted to 11 affiliated NICUs from 2010 to 2013. The clinical information and laboratory data were registered in a network database designed for this study. The RIs for 26 items were derived using the parametric method after applying the latent abnormal values exclusion method. The influence of birth weight (BW) and gestational age (GA) on the test results was expressed in terms of the standard deviation ratio (SDR), as SDRBW and SDRGA, respectively. A total of 3189 infants were admitted during the study period; 246 were excluded due to a lack of blood sampling data, and 234 were excluded for chromosomal abnormalities (n = 108), congenital anomalies requiring treatment with surgical procedures (n = 76), and death or transfer to another hospital (n = 50). As a result, 2709 infants were enrolled in this study. Both the SDRGA and SDRBW were above 0.4 in the test results for total protein (TP), albumin (ALB), alanine aminotransferase (ALT), and red blood cells (RBC); their values increased in proportion to the BW and GA. We derived 26 blood RIs for infants who were admitted to NICUs. These RIs should help in the performance of proper clinical assessments and research in the field of perinatal-neonatal medicine.

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“…Changes in WBC counts and function have been previously reported in neonatal CF‐1 and BALB/C mice . While lymphocyte counts can rise due to increased production, mechanisms behind leukocyte tissue sequestration and destruction contributing to the shifts in neutrophil/lymphocyte ratios during neonatal development are currently unclear. Another small limitation of this study is the lack of WBC differential count validation based on blood smear evaluation.…”

Section: Sysmex Xt‐2000iv Performance and Capabilities Performance Cmentioning

confidence: 98%

Evaluation of hematologic variables in newborn C57/BL6 mice up to day 35

White

Gong

Colaizy

et al. 2015

Veterinary Clinical Pathol

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Background Hematologic variables are often analyzed in animal analogues during the investigation of complex disease etiologies such as necrotizing enterocolitis. However, reference intervals (RI) can vary depending on animal strain, age, and sampling site. Reference intervals have been published for adult C57BL/6J mice, but not newborn C57BL/6J mice. Objectives The purpose of the present study was to determine hematologic RI in newborn C57BL/6J mice up to day 35. Methods C57BL/6J mice founders from The Jackson Laboratory were bred at the University of Iowa. Blood samples were obtained via facial vein sampling at postnatal days 0 (p0), p7, p14, p21, p28, or young adulthood (p35). CBCs were determined with the Sysmex XT-2000iV analyzer within 30 minutes of blood collection at a 1:10 dilution. Statistics were determined using nonparametric methods following ASVCP guidelines. Results Hematologic RI were determined for each of the 6 groups (n=247, n≥39 per group). Significantly higher values for HGB, RBC, and PLT counts were observed with advancing developmental age. Total WBC counts remained relatively stable during the first 35 days of life. However, WBC differential counts were dominated by neutrophils and lymphocytes in the younger mice, with a trend towards a lymphocytic leukogram on day 35. Conclusions These results illustrate the dynamic changes in hematologic variables during murine development after birth. Utilization of age-specific RI is advised when evaluating data derived from experimental perinatal mouse models.

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Reference Ranges for Lymphocyte Counts of Neonates: Associations Between Abnormal Counts and Outcomes

Abstract: A reference range for lymphocytes can identify neonates with abnormal counts, which can be useful because these neonates are at higher risk for certain adverse outcomes.

Cited by 37 publications

References 24 publications

A comprehensive study of umbilical cord blood cell developmental changes and reference ranges by gestation, gender and mode of delivery

A comprehensive study of umbilical cord blood cell developmental changes and reference ranges by gestation, gender and mode of delivery

Blood Reference Intervals for Preterm Low-Birth-Weight Infants: A Multicenter Cohort Study in Japan

Evaluation of hematologic variables in newborn C57/BL6 mice up to day 35

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