Reference Materials as a Crucial Tools for Validation and Verification of the Analytical Process

Zakaria, Osman; Rezali, Mohd Fazlin

doi:10.1016/j.sbspro.2014.01.1121

Cited by 10 publications

(6 citation statements)

References 10 publications

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“…This evaluation of performance should be based on challenging the pipeline with input representing a carefully selected set of resistance determinants and bacterial hosts. These sets of NGS reads should be fully characterised regarding their genetic content and serve as ( in silico ) reference materials for the validation and quality control of the bioinformatics component of the methods (see, for other host models, 66 , 67 ).…”

Section: Design Of the Scope-specific Benchmark Resourcesmentioning

confidence: 99%

A roadmap for the generation of benchmarking resources for antimicrobial resistance detection using next generation sequencing

et al. 2022

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Next Generation Sequencing technologies significantly impact the field of Antimicrobial Resistance (AMR) detection and monitoring, with immediate uses in diagnosis and risk assessment. For this application and in general, considerable challenges remain in demonstrating sufficient trust to act upon the meaningful information produced from raw data, partly because of the reliance on bioinformatics pipelines, which can produce different results and therefore lead to different interpretations. With the constant evolution of the field, it is difficult to identify, harmonise and recommend specific methods for large-scale implementations over time. In this article, we propose to address this challenge through establishing a transparent, performance-based, evaluation approach to provide flexibility in the bioinformatics tools of choice, while demonstrating proficiency in meeting common performance standards. The approach is two-fold: first, a community-driven effort to establish and maintain “live” (dynamic) benchmarking platforms to provide relevant performance metrics, based on different use-cases, that would evolve together with the AMR field; second, agreed and defined datasets to allow the pipelines’ implementation, validation, and quality-control over time. Following previous discussions on the main challenges linked to this approach, we provide concrete recommendations and future steps, related to different aspects of the design of benchmarks, such as the selection and the characteristics of the datasets (quality, choice of pathogens and resistances, etc.), the evaluation criteria of the pipelines, and the way these resources should be deployed in the community.

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Section: Design Of the Scope-specific Benchmark Resourcesmentioning

confidence: 99%

A roadmap for the generation of benchmarking resources for antimicrobial resistance detection using next generation sequencing

et al. 2022

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“…Firstly, CRMs enable the assessment of a method trueness which is also known as bias. By comparing the measured concentration of the analyte in the CRM with its certified value, we can evaluate if the method consistently yields accurate results [24]. This paper presents a comprehensive validation of an HS-GC-FID method for the measurement of ethanol in water utilizing a NIST standard reference material.…”

Section: Introductionmentioning

confidence: 99%

Validation of a Method for Characterization of Ethanol in Water by HS-GC-FID to Serve the Traceability of Halal Measurements

Shehata,

Askar,

Rasheed

et al. 2024

GSC

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The determination of the ethanol content in food products is of fundamental importance for HALAL certification. In this work, an analytical method for the determination of ethanol in water by headspace gas chromatography with flame ionization detector (HS-GC-FID) has been developed and validated for the use in characterization of ethanol reference materials. The validation study was carried out in the linear calibration range 100 -1500 mg/kg using the NIST SRM 2900, nominal 95.6%. The studied performance characteristics of the method were the limit of detection, LOD, the limit of quantification LOQ, selectivity, linearity, precision, recovery and bias. The validation results showed that the method is selective, precise, accurate and free from any significant bias. The LOD and LOQ were 1.27 and 3.86 mg/kg respectively and the estimated expanded uncertainty was 2% indicating that the method is fit for the purpose of certification of ethanol in water reference materials.

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“…In this sense, it is clear that reference materials or available standards could be of substantial importance to control method performance and maintain the reliability of the data produced (International atomic energy agency, 2003). However, the high cost for CRM production is a factor that influences the development of new reference materials (Lauwaars and Anklam, 2005;Zakaria and Rezali, 2014). Therefore, it is important to carry out research to produce new stable materials, characterized by their low cost, availability, and high concentration of easily extractable carotenoid components.…”

Section: Introductionmentioning

confidence: 99%

Interlaboratory exercise for the analysis of carotenoids and related compounds in dried mango fruit (Mangifera indica L.)

Villacís-Chiriboga

Jacobs

Camp

et al. 2022

Journal of Food Composition and Analysis

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Reference Materials as a Crucial Tools for Validation and Verification of the Analytical Process

Cited by 10 publications

References 10 publications

A roadmap for the generation of benchmarking resources for antimicrobial resistance detection using next generation sequencing

A roadmap for the generation of benchmarking resources for antimicrobial resistance detection using next generation sequencing

Validation of a Method for Characterization of Ethanol in Water by HS-GC-FID to Serve the Traceability of Halal Measurements

Interlaboratory exercise for the analysis of carotenoids and related compounds in dried mango fruit (Mangifera indica L.)

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