Reference intervals for haematological and plasma biochemical parameters in sobaity sea bream juveniles (Sparidentex hasta, Valenciennes 1830)

Abstract

Sobaity sea bream (Sparidentex hasta) is distributed in the Western Indian Ocean, Persian Gulf and Oman Sea. For monitoring health status in this species in an aquaculture condition, standard reference values for haematological and plasma biochemical parameters are indispensable. Heparinized blood samples were collected from 143 healthy sobaity sea bream juveniles raised in cylindrical polyethylene tanks with filtered running seawater. Haematologic reference intervals were as follows: red blood cell (RBC) count 1.67-2.2× 10 6 μL −1 , total white blood cell (WBC) count 8.8-14.5× 10 3 μL −1 , lymphocytes 85-96 %, neutrophils 2-6 %, haemoglobin 28-60 g L −1 , haematocrit (Hct) 24.5-33.8 %, mean cell volume (MCV) 126.3-191.7 fL, mean cell haemoglobin (MCH) 14.4-26.5 pg, mean cell haemoglobin concentration (MCHC) 92-180 g L −1 , erythrocyte sedimentation rate (ESR) 1.5-5 mm h −1 and RBC/WBC ratio 119.2-207.5. Plasma chemistry reference intervals were as follows: total protein 3.2-3.9 g dL −1 , albumin 0.3-0.58 g dL −1 , globulin 2.7-3.4 g dL −1 , alkaline phosphatase 444-866 U L −1 , alanine aminotransferase 239-506.5 U L −1 , lipase 95.5-120 U L −1 , amylase 4-8 U L −1 , sodium 173-211 mmol L −1 , chloride 103-136.5 mmol L −1 , potassium 2.7-4.4 mmol L −1 , calcium 2.6-3.1 mmol L −1 , inorganic phosphorus 2.9-5.3 mmol L −1 , osmolality 430-503 mOsm kg −1 , glucose 6.16-7.88 mmol L −1 , cholesterol 4.07-7.7 mmol L −1 , triglyceride 2.22-3.91 mmol L −1 , high-density lipoprotein (HDL) 1.36-2.44 mmol L −1 , low-density lipoprotein (LDL) 0.84-1.19 mmol L −1 and very-low-density lipoprotein (VLDL) 0.27-0.78 mmol L −1 . Values reported here will be useful for evaluating the health status, interpretation and better understanding of various disease signs in sobaity sea bream juveniles.

Partial or total replacement of dietary fish oil with alternative lipid sources in silvery-black porgy (Sparidentex hasta)

“…Under current experimental conditions, differences in dietary FA resulted in changes in hemoglobin levels and RBC among groups, even though they were within the normal range of values reported for healthy juvenile of silvery-black porgy (Mozanzadeh et al, 2015b). The reasons for such differences among groups are unclear and deserve further attention.…”

Optimal dietary carbohydrate-to-lipid ratios for silvery-black porgy (Sparidentex hasta) juveniles

“…Protein, total protein, albumin and globulin levels were not influenced by different dietary CHO:L ratios and values were within the normal range of values reported for this species (Mozanzadeh et al . ). Except for HDL ( P < 0.05), all other lipid components of plasma such as cholesterol, triglyceride and LDL were not affected in silvery‐black porgy fed with different CHO:L ratio ( P > 0.05) diets.…”

“…In the current study, the plasma concentration of ALP and ALT, biomarkers of liver health condition, decreased with increasing dietary CHO:L ratios ( P < 0.05), and values in fish fed with the 0.3 and 0.6 CHO:L ratio diets were greater than those reported for healthy juvenile fish (Mozanzadeh et al . ). Dietary CHO:L ratios had also a significant and negative correlation to plasma ALP ( r = −0.819; P = 0.001) and ALT ( r = −0.732; P = 0.007) levels.…”

“…The results of the current study showed that silvery‐black porgy fed with 1.1 and 1.8 CHO:L ratio diets had higher RBC than other groups and these values were higher than those reported for healthy juvenile fish (Mozanzadeh et al . ), which may indicate metabolic burden in these groups. There was also a positive correlation between RBC counts and dietary CHO:L ratios ( r = 0.773; P = 0.003).…”

Dietary n−3 LC-PUFA requirements in silvery-black porgy juveniles (Sparidentex hasta)

“…In recent years, this species has been regularly propagated in the Mariculture Research Station of the South Iranian Aquaculture Research Center (SIARC, Sarbandar, Iran). Juvenile fish have subsequently been released into the Persian Gulf to increase stock numbers or transferred to sea cages for marine fish aquaculture extension projects (Mozanzadeh et al, 2015). In 2011, an estimated 550 metric tons of silvery-black porgy was produced from farming activities in the region (FAO, 2014).…”