Reference Intervals for Blood Analytes of Adult Aquarium‐Housed Russian Sturgeon Acipenser gueldenstaedtii

Abstract

Russian Sturgeon Acipenser gueldenstaedtii are an important, critically endangered, roe‐producing species. Despite a wealth of knowledge pertaining to other members of family Acipenseridae, there is very limited published information regarding baseline blood analytes in Russian Sturgeon. The objectives of this study were (1) to establish reference intervals for a suite of hematological and biochemical data and (2) to compare plasma chemistry data to two point‐of‐care (POC) cartridges, tested on the VetScan iSTAT 1 analyzer, that use heparinized whole blood for the assessment of clinically normal, aquacultured adult Russian Sturgeon sedated with eugenol (AQUI‐S 20E) at a single institution. Reference intervals are reported. The calculated hematocrit measured by the POC analyzer tended 4–5% lower than the spun packed cell volume, confirming the importance of spun packed cell volume as a reliable measurement of red blood cell mass. Various analytes, notably whole‐blood urea nitrogen, glucose, sodium, total carbon dioxide, chloride, ionized calcium, and anion gap, were significantly different by both POC cartridges. This study successfully produced reference intervals for blood analytes in adult Russian Sturgeon under managed care and creates a foundation for future studies into the effects of extrinsic and intrinsic factors and variations of analytical methodologies on blood analytes in this species.

First Isolation of a Herpesvirus (Family Alloherpesviridae) from Great Lakes Lake Sturgeon (Acipenser fulvescens)

“…No clear trends in PCV were appreciable by site or year ( Table 2 ); however, female lake sturgeon had lower mean PCVs in both year one and two at the Black River site when compared to males ( Table 2 ). PCV measurements were comparable to reference intervals established by DiVincenti et al (2013) and Cassle et al (2013) [ 48 , 49 ].…”

“…There is little published information regarding the relationships between hematologic parameters, such as packed cell volume, and alloherpesvirus infection. Overall, this study found no substantial differences in PCV between male and female fish from any site in any year, and all PCV averages fell within published sturgeon ( A. fulvescens and A. gueldenstaiedtii ) reference intervals (17–38% and 16–34%) [ 48 , 49 ].…”

Indexing serum and mucous biochemical parameters of endangered Chinese sturgeon Acipenser sinensis with implications for health assessment

“…This study establishes blood biochemical RIs for Chinese sturgeon and is an essential first step in the application of these parameters to Aramli et al, 2013;Asadi et al, 2006;Cassle et al, 2021;Di Marco et al, 2011;DiVincenti Jr. et al, 2013;Matsche et al, 2013;Matsche et al, 2014;Shahsavani et al, 2010;Shi et al, 2006). Compared to other sturgeons, there are fewer studies on Chinese sturgeon that can be directly compared to our study.…”

Determination of Reference Intervals for Hematological Parameters in Cultured Diamond Sturgeon (Acipenser gueldenstaedtii)

“…Ancak balıklarda referans değerlerini belirlemek son derece zordur ve çoğu kan parametresinin normal aralıkları, balığın iç ortamının poikilotermiye bağlı kararsızlığı ve dış etkenlere karşı yüksek duyarlılığı nedeniyle çok geniştir (Luskova, 1997;Witeska, 2015). Bu nedenle yapılacak çalışmalarda referans aralıklarını cinsiyet, yaş, cinsi olgunluk durumu gibi biyotik ve abiyotik faktörlere göre belirlemek bir tanı aracı olarak hematolojik ve biyokimyasal analizlerin doğruluğunu ve kullanışlılığını artırır (Cassle, 2021). Bununla birlikte, numune alım tekniklerinin ve kan analizlerinin standardize edilmesi yaklaşımı da hematolojik ve biyokimyasal analizlerin doğruluğunu destekleyici nitelikte olacaktır (Fazio, 2019).…”