Reference intervals for absolute and percentage immature platelet fraction using the Sysmex XN-10 automated haematology analyser in a UK population

Ali, Usman; Knight, Gavin; Gibbs, Roz; Tsitsikas, Dimitris A.

doi:10.1080/00365513.2017.1394488

Cited by 14 publications

(14 citation statements)

References 52 publications

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“…The data for males and females were also treated as a single entity when considering the IPF%. The normal reference range established in the present study was 1.3-12.8%, which was in keeping with that derived by Ali et al (2017) [21]. However Ali et al did report on small but significant differences in IPF% between healthy males and females, and therefore derived two separate reference ranges.…”

Section: Resultssupporting

confidence: 92%

“…However Ali et al did report on small but significant differences in IPF% between healthy males and females, and therefore derived two separate reference ranges. The reason for the different findings between studies may simply be down to sample size, with the present study deriving the reference range from 171 controls, while Ali et al derived from a control population of 2366 [21].…”

Section: Resultsmentioning

confidence: 66%

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Immature platelet fraction as a useful marker in the etiological determination of thrombocytopenia

Ali

Graham

Dempsey-Hibbert

2019

Experimental Hematology

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Background: The aetiology of thrombocytopenia is important in treatment and management of the condition. Most platelet parameters that are routinely analysed in the diagnostic laboratory have not proven useful in identifying the aetiology while specialised assays suffer from poor standardisation and lack of agreement between laboratories. The immature platelet fraction (IPF), which indirectly provides a measure of bone marrow function is showing promise as a valuable marker of thrombopoietic responses. Objectives: To determine whether the IPF could effectively identify specific underlying aetiologies of thrombocytopenia in a large thrombocytopenic cohort, to allow for quicker, more effective management of the condition. Patients and Methods: The IPF was analysed in a large cohort of 637 thrombocytopenic patients and 171 healthy control patients on the Sysmex XN 10 haematology analyser using the specialised fluorescence optical analysis. The thrombocytopenic patients were divided into six cohorts based on aetiology. Results: The IPF%, was significantly higher in cases of increased platelet consumption (median=9.55, min=1.1, max= 77.9), or pseudothrombocytopenia (median=13.1, min=0.4, max=28.8) compared with control (median=4.2, min=1.3, max=12.8). Furthermore, the IPF% was also able to identify idiopathic thrombocytopenic purpura (ITP) (P<0.05) (median=13.4, min=2.8, max=77.9) from other causes of increased platelet consumptive disorders (Infection; median=6.4, min=1.1, max=21.6; haemorrhage, median=8.9, min=1.2, max=20.2). Conclusion: Using this large thrombocytopenic cohort, the IPF% has been shown to be of significant diagnostic value, providing a useful rapid test in the aetiological investigation of platelet disorders.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 66%

Immature platelet fraction as a useful marker in the etiological determination of thrombocytopenia

Ali

Graham

Dempsey-Hibbert

2019

Experimental Hematology

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“…Their results were consistent with an impact of smoking in a covariance model on platelet volume and IPF, 43 while Butkiewicz et al have found a significant higher percentage of reticulated platelet among smoking women compared to nonsmokers. 45,46 Platelet count was found slightly higher among smokers, even if that difference was not statistically significant, but immature platelet count was statistically significant higher in smokers. 45 In our study, conducted among patients undergoing coronary angiography, we found higher IPF among active smokers as compared to the nonactive ones, who were considered comparable to a "normal" control population, since comprising a heterogeneous and largely sized cohort of patients, including patients with and without CAD.…”

Section: Discussionmentioning

confidence: 74%

“…In fact, although we included also patients admitted for an acute coronary syndrome, the IPF% of our cohort was similar to the values those reported by other studies on European cohorts. 45,46 Platelet count was found slightly higher among smokers, even if that difference was not statistically significant, but immature platelet count was statistically significant higher in smokers. Moreover, smokers showed lower prevalence and diabetes, hypertension and renal failure and less pharmacological therapies.…”

Section: Discussionmentioning

confidence: 74%

Impact of active smoking on the immature platelet fraction and its relationship with the extent of coronary artery disease

Nardin

Verdoia

Negro³

et al. 2020

Eur J Clin Investigation

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Introduction:Smoking represents a major cardiovascular risk factor, due to the induction of oxidative stress and low-grade, continuous, inflammation that contribute to promote atherothrombosis. However, the mechanisms leading to increased platelet aggregability associated with smoking are only partially defined. A potential role has been hypothesized for immature platelets, a younger and potentially more reactive fraction, previously associated with the main determinants of coronary artery disease (CAD). Therefore, the aim of our study was to define the impact of smoking on the immature platelet fraction (IPF) and its relationship with prevalence and extent of coronary artery disease. Methods: We enrolled a cohort of consecutive patients undergoing coronary angiography in a single centre. Significant CAD was defined as at least 1 vessel stenosis >50%, while severe CAD was defined as left main and/or three-vessel disease. IPF was measured at admission by routine blood cell count (Sysmex XE-2100). Results: We included in our study 2553 patients who were divided according to smoking status (active smokers: 512; nonactive smokers: 2041). Smokers were younger, more frequent males, with lower rate of diabetes mellitus, previous PCI and previous CABG (P < .001, respectively) and were in treatment less often with ARB, BB, nitrates, statins, ASA, clopidogrel, CCB and diuretics (P < .001, respectively) as compared to nonactive smokers. Higher percentage of smokers was observed in patients with higher IPF values, and at multivariate analysis, active smoking resulted as an independent predictor of higher IPF (adjusted OR [95% CI] = 1.59[1.03-2.45], P = .035). Among smokers, higher IPF was associated with lower ejection fraction (P = .034), percentage of acute coronary syndrome (P = .002) and platelet count (P < .001) compared to ones with lower IPF. However, the IPF (according to quartiles values) was not associated with the prevalence and extent of CAD (82.5%, 80.4%, 86.1% and 80.9%, from 1st to 4th quartile, respectively, adjusted OR[95% CI] = 0.98[0.79-1.23], P = .89) and severe CAD (31%, 31.1%, 39.1% and 35.2%, from 1st to 4th quartile, respectively, adjusted OR[95% CI] = 1.03[0.86-1.23], P = .76).

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“…The fluorescent dye is CD4K530 for Abbott analyzers and oxazine-based for Sysmex analyzers. Despite the poor standardization and a lack of correlation between the young platelet fraction measurements among the different instruments (absence of reference method and the existence of different reference ranges for different instruments), these parameters are good indicators of thrombopoietic activity [20][21][22]. IPF absolute count has been proven as an indicator of platelet recovery after chemotherapy in pediatrics [23] and, thus, has been proposed as a tool to categorize neonatal thrombocytopenia [24] or to predict peripheral immune thrombocytopenia [25,26].…”

Section: Fluorescence Platelet Countingmentioning

confidence: 99%

Platelet Counting: Ugly Traps and Good Advice. Proposals from the French-Speaking Cellular Hematology Group (GFHC)

Baccini

Geneviève

Jacqmin

et al. 2020

JCM

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Despite the ongoing development of automated hematology analyzers to optimize complete blood count results, platelet count still suffers from pre-analytical or analytical pitfalls, including EDTA-induced pseudothrombocytopenia. Although most of these interferences are widely known, laboratory practices remain highly heterogeneous. In order to harmonize and standardize cellular hematology practices, the French-speaking Cellular Hematology Group (GFHC) wants to focus on interferences that could affect the platelet count and to detail the verification steps with minimal recommendations, taking into account the different technologies employed nowadays. The conclusions of the GFHC presented here met with a "strong professional agreement" and are explained with their rationale to define the course of actions, in case thrombocytopenia or thrombocytosis is detected. They are proposed as minimum recommendations to be used by each specialist in laboratory medicine who remains free to use more restrictive guidelines based on the patient's condition.

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Reference intervals for absolute and percentage immature platelet fraction using the Sysmex XN-10 automated haematology analyser in a UK population

Cited by 14 publications

References 52 publications

Immature platelet fraction as a useful marker in the etiological determination of thrombocytopenia

Immature platelet fraction as a useful marker in the etiological determination of thrombocytopenia

Impact of active smoking on the immature platelet fraction and its relationship with the extent of coronary artery disease

Platelet Counting: Ugly Traps and Good Advice. Proposals from the French-Speaking Cellular Hematology Group (GFHC)

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