Refeeding meal-fed rats increases lipoprotein lipase activity and deposition of dietary [14C]lipid in white adipose tissue and decreases oxidation to 14CO2. The role of undernutrition

Cruz, Martha L.; Williamson, Dermot H.

doi:10.1042/bj2850773

Cited by 14 publications

(3 citation statements)

References 29 publications

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“…Although previous studies have analysed the possible effects of plasma glucose, glucagon, glucagon:insulin ratio and catecholamines (Borensztajn, 1987), the factors which mediate dietary action on muscle-LPL gene expression and activity has not been identified in any animal species. LPL activity was much greater in perirenal than in subcutaneous AT, in keeping with results reported for cattle (Hocquette et al 1998), goat (Chilliard et al 1981) and rat (Cruz & Williamson, 1992) AT. Furthermore, LPL activity was greater in cardiac (oxidative) than in skeletal (glycolytic) muscle as described in bovine (Hocquette et al 1998) and rat (Ladu et al 1991;Ong et al 1994) muscles.…”

Section: Effect Of Feeding Level On Activities Of Lipogenic Enzymes Asupporting

confidence: 87%

Effects of photoperiod and feeding level on adipose tissue and muscle lipoprotein lipase activity and mRNA level in dry non-pregnant sheep

et al. 2001

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The aim of the present study was to investigate the effects of photoperiod and feeding level on lipid metabolism in ovine perirenal and subcutaneous adipose tissues (AT) and in skeletal and cardiac muscles. Twenty dry non-pregnant ovariectomised ewes were divided into two groups and subjected to either 8 h or 16 h light/d, and underfed at 22 % energy requirements for 7 d. Half of the ewes in each group were slaughtered and the remaining ewes were refed at 190 % energy requirements for 14 d, until slaughtering. Refeeding increased (2´6±4´3-fold) malic enzyme (ME), fatty acid synthase (FAS), glucose-6-phosphate dehydrogenase (G6PDH) and glycerol-3-phosphate dehydrogenase (G3PDH) activities in subcutaneous AT as well as lipoprotein lipase (LPL) activity in perirenal (3´5-fold) and subcutaneous (10-fold) AT and to a lesser extent (1´4-fold) in the skeletal longissimus thoracis and cardiac muscles. Moreover, variations of LPL mRNA level followed variations of LPL activity: refeeding increased perirenal AT-and cardiac muscle-mRNA levels (7´4-and 2-fold respectively). The main finding of this study is that, for a given level of food intake, long days (compared with short days) increased the LPL activity in the longissimus thoracis muscle and, in refed ewes, the activities of LPL and ME in subcutaneous AT. Furthermore, long days increased LPL mRNA level in cardiac muscle and perirenal AT. Thus, our results show that there are direct effects of photoperiod on sheep AT lipogenic potential, as well as on muscle LPL activity, which are not caused by changes in nutrient availability.

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Section: Effect Of Feeding Level On Activities Of Lipogenic Enzymes Asupporting

confidence: 87%

Effects of photoperiod and feeding level on adipose tissue and muscle lipoprotein lipase activity and mRNA level in dry non-pregnant sheep

et al. 2001

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“…Activity of the adipocyte enzyme lipoprotein lipase (LPL;EC 3.1.1.34) is an important determinant of the amount of triacylglycerol stored in adipose tissue (Eckel, 1989;Liu et al, 2006;Choi et al, 2007). LPL activity in adipose tissue increases several-fold within a few hours of ingestion of a meal (Eckel, 1989;Cruz and Williamson, 1992) and decreases progressively with fasting (Cryer, 1981;Lien and Jan, 2003;Wu et al, 2003). However, the mechanisms by which fasting decreases LPL activity are not clear.…”

Section: Introductionmentioning

confidence: 99%

Regulation of Lipoprotein Lipase by Fasting in Epididymal and Mesenteric Adipocytes of Rats

Lee¹,

Chung²,

Lee³

2008

Asian Australas. J. Anim. Sci

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There are marked variations in the activity of lipoprotein lipase (LPL) among adipose depots. The aim of this study was to compare the mechanisms of 24 h of fasting on LPL regulation between epididymal (EPI) adipocytes and mesenteric (MES) adipocytes in rats. 1-Day fasting consistently decreased activities of heparin-releasable LPL, total extractable LPL and cellular LPL markedly in both EPI and MES fat pads. LPL activity in MES fat pads was relatively lower than in the EPI fat pads. Consistent with data on LPL activity, the levels of expression of LPL mRNA in both nutritional states were lower in MES than EPI adipose tissue and isolated adipocytes. The decreased LPL activity after 1 day of fasting in MES adipocytes was explained mainly by a 50% decrease in the relative abundance of LPL mRNA level and a parallel 50% decrease in relative rate of LPL synthesis. In contrast, fasting of 1 day in EPI adipocytes decreased total LPL activity by 47% but did not affect LPL mRNA level or relative rate of LPL synthesis. A decrease in overall protein synthesis contributed to the decreased LPL activity after 1 day fasting both in EPI and MES adipocytes. In MES adipocytes the decrease in LPL activity, LPL mRNA and LPL synthesis were comparable, but in EPI adipocytes the changes in LPL activity were substantially larger than the changes in LPL mRNA level and LPL synthesis. Therefore, fasting decreased fat cell size, LPL activity, LPL mRNA level and relative rate of LPL synthesis in rats, and these effects were more marked in the MES adipocytes. These results clearly demonstrate the regional variations in the metabolic response of adipose tissue and LPL functions to fasting.

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“…While animals are thus trained to eat during this 2-hour period, they also develop a condition of food restriction [3,5,10,11,33]. Because the amount of food intake is not comparable to that over 24 h, MFS rats present hyperphagia, gastric hypertrophy and increased gastric capacity [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Effect of a Meal Feeding Schedule on Hepatic Glycogen Synthesis and Gluconeogenesis in Rats

Batista

Curi

Lima

et al. 2001

Journal of Biomedical Science

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We investigated the effect of a meal feeding schedule (MFS) on food intake, hepatic glycogen synthesis, hepatic capacity to produce glucose and glycemia in rats. The MFS comprised free access to food for a 2-hour period daily at a fixed mealtime (8.00–10.00 a.m.) for 13 days. The control group was composed of rats with free access to food from day 1 to 12, which were then starved for 22 h, refed with a single meal at 8.00–10.00 a.m. and starved again for another 22 h. All experiments were performed at the meal time (i.e. 8.00 a.m.). The MFS group exhibited increased food intake and higher glycogen synthase activity. Since gluconeogenesis from L-glutamine or L-alanine was not affected by MFS, we conclude that the increased food intake and higher glycogen synthase activity contributed to the better glucose maintenance showed by MFS rats at the fixed meal time.

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Refeeding meal-fed rats increases lipoprotein lipase activity and deposition of dietary [14C]lipid in white adipose tissue and decreases oxidation to 14CO2. The role of undernutrition

Cited by 14 publications

References 29 publications

Effects of photoperiod and feeding level on adipose tissue and muscle lipoprotein lipase activity and mRNA level in dry non-pregnant sheep

Effects of photoperiod and feeding level on adipose tissue and muscle lipoprotein lipase activity and mRNA level in dry non-pregnant sheep

Regulation of Lipoprotein Lipase by Fasting in Epididymal and Mesenteric Adipocytes of Rats

Effect of a Meal Feeding Schedule on Hepatic Glycogen Synthesis and Gluconeogenesis in Rats

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