REF6 recognizes a specific DNA sequence to demethylate H3K27me3 and regulate organ boundary formation in Arabidopsis

Cui, Xia; Lu, Falong; Qiu, Qi; Zhou, Bing; Gu, Lianfeng; Zhang, Shuaibin; Kang, Yongshui; Cui, Xiekui; Ma, Xuan; Yao, Qingqing; Ma, Jinbiao; Zhang, Xiaoyu; Cao, Xiaofeng

doi:10.1038/ng.3556

Cited by 144 publications

(150 citation statements)

References 44 publications

Supporting

Mentioning

141

Contrasting

Unclassified

Order By: Relevance

“…Samples were incubated at 4°C for 10 min for DNA-protein cross-linking. To quench the cross-linking 2 M glycine was added, then samples were incubated at 4°C for 5 min (Cui et al, 2016). The chromatin was extracted twice in ChIP extraction buffer 2 (Bowler et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

Autocrine regulation of stomatal differentiation potential by EPF1 and ERECTA-LIKE1 ligand-receptor signaling

Han

Dang

et al. 2017

eLife

View full text Add to dashboard Cite

Development of stomata, valves on the plant epidermis for optimal gas exchange and water control, is fine-tuned by multiple signaling peptides with unique, overlapping, or antagonistic activities. EPIDERMAL PATTERNING FACTOR1 (EPF1) is a founding member of the secreted peptide ligands enforcing stomatal patterning. Yet, its exact role remains unclear. Here, we report that EPF1 and its primary receptor ERECTA-LIKE1 (ERL1) target MUTE, a transcription factor specifying the proliferation-to-differentiation switch within the stomatal cell lineages. In turn, MUTE directly induces ERL1. The absolute co-expression of ERL1 and MUTE, with the co-presence of EPF1, triggers autocrine inhibition of stomatal fate. During normal stomatal development, this autocrine inhibition prevents extra symmetric divisions of stomatal precursors likely owing to excessive MUTE activity. Our study reveals the unexpected role of self-inhibition as a mechanism for ensuring proper stomatal development and suggests an intricate signal buffering mechanism underlying plant tissue patterning.DOI: http://dx.doi.org/10.7554/eLife.24102.001

show abstract

Section: Methodsmentioning

confidence: 99%

Autocrine regulation of stomatal differentiation potential by EPF1 and ERECTA-LIKE1 ligand-receptor signaling

Han

Dang

et al. 2017

eLife

View full text Add to dashboard Cite

show abstract

“…Most histone modification enzymes have not been observed to bind DNA, except for REF6 and MLL1, which contain a zinc finger domain and CXXC domain, respectively (Cierpicki et al, 2010;Cui et al, 2016). The fundamental question of how these histone modification proteins target specific genes to carry out their functions remains to be explored.…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation of Histone H2A at Serine 95: A Plant-Specific Mark Involved in Flowering Time Regulation and H2A.Z Deposition

Wang

Zhang

et al. 2017

Plant Cell

View full text Add to dashboard Cite

Phosphorylation of histone H3 affects transcription, chromatin condensation, and chromosome segregation. However, the role of phosphorylation of histone H2A remains unclear. Here, we found that Arabidopsis thaliana MUT9P-LIKE-KINASE (MLK4) phosphorylates histone H2A on serine 95, a plant-specific modification in the histone core domain. Mutations in MLK4 caused late flowering under long-day conditions but no notable phenotype under short days. MLK4 interacts with CIRCADIAN CLOCK ASSOCIATED1 (CCA1), which allows MLK4 to bind to the GIGANTEA (GI) promoter. CCA1 interacts with YAF9a, a co-subunit of the Swi2/Snf2-related ATPase (SWR1) and NuA4 complexes, which are responsible for incorporating the histone variant H2A.Z into chromatin and histone H4 acetylase activity, respectively. Importantly, loss of MLK4 function led to delayed flowering by decreasing phosphorylation of H2A serine 95, along with attenuated accumulation of H2A.Z and the acetylation of H4 at GI, thus reducing GI expression. Together, our results provide insight into how phosphorylation of H2A serine 95 promotes flowering time and suggest that phosphorylation of H2A serine 95 modulated by MLK4 is required for the regulation of flowering time and is involved in deposition of the histone variant H2A.Z and H4 acetylation in Arabidopsis.

show abstract

“…These prior works have clearly demonstrated the importance of CLF and SWN in plant development, but mostly based on mutant examination and single gene analyses. Meanwhile, although a number of H3K27me3 genome‐wide profiling have been reported (Carter et al., ; Cui et al., ; Li et al., , ; Luo et al., ; Wang et al., ; Yang et al., ), works on genome‐wide occupancy of the CLF and SWN proteins are rather limited. The recently published CLF ChIP‐chip data were based on a transgenic line in which CLF was driven by a constitutive promoter (Xiao et al., ).…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide occupancy of histone H3K27 methyltransferases CURLY LEAF and SWINGER in Arabidopsis seedlings

et al. 2019

View full text Add to dashboard Cite

The Polycomb Group (PcG) proteins form two protein complexes, PcG Repressive Complex 1 ( PRC 1) and PRC 2, which are key epigenetic regulators in eukaryotes. PRC 2 represses gene expression by catalyzing the trimethylation of histone H3 lysine 27 (H3K27me3). In Arabidopsis ( Arabidopsis thaliana ), CURLY LEAF ( CLF ) and SWINGER ( SWN ) are two major H3K27 methyltransferases and core components of PRC 2, playing essential roles in plant growth and development. Despite their importance, genome‐wide binding profiles of CLF and SWN have not been determined and compared yet. In this study, we generated transgenic lines expressing GFP ‐tagged CLF / SWN under their respective native promoters and used them for Ch IP ‐seq analyses to profile the genome‐wide distributions of CLF and SWN in Arabidopsis seedlings. We also profiled and compared the global H3K27me3 levels in wild‐type ( WT ) and PcG mutants ( clf , swn , and clf swn ). Our data show that CLF and SWN bind to almost the same set of genes, except that SWN has a few hundred more targets. Two short DNA sequences, the GAGA ‐like and Telo ‐box‐like motifs, were found enriched in the CLF and SWN binding regions. The H3K27me3 levels in clf , but not in swn , were markedly reduced compared with WT ; and the mark was undetectable in the clf swn double mutant. Further, we profiled the transcriptomes in clf , swn , and clf swn, and compared that with WT . Thus this work provides a useful resource for the plant epigenetics community for dissecting the functions of PRC 2 in plant growth and development.

show abstract

REF6 recognizes a specific DNA sequence to demethylate H3K27me3 and regulate organ boundary formation in Arabidopsis

Cited by 144 publications

References 44 publications

Autocrine regulation of stomatal differentiation potential by EPF1 and ERECTA-LIKE1 ligand-receptor signaling

Autocrine regulation of stomatal differentiation potential by EPF1 and ERECTA-LIKE1 ligand-receptor signaling

Phosphorylation of Histone H2A at Serine 95: A Plant-Specific Mark Involved in Flowering Time Regulation and H2A.Z Deposition

Genome‐wide occupancy of histone H3K27 methyltransferases CURLY LEAF and SWINGER in Arabidopsis seedlings

Contact Info

Product

Resources

About