Reelin‐Dab1 signaling system in human colorectal cancer

Serrano-Morales, José Manuel; Vázquez‐Carretero, María D.; Peral, María J.; Ilundáin, A.; García‐Miranda, Pablo

doi:10.1002/mc.22527

Cited by 22 publications

(28 citation statements)

References 37 publications

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“…Opto-Dab1 may also be useful to study Reelin-Dab1 signaling in synaptic plasticity140, in the development of non-neuronal tissues, such as mammary gland, small intestine and limbs, and in diseases including cancer414243. These applications will require the development of techniques to express opto-Dab1 effectively in the target cell types and to direct light to the respective tissues.…”

Section: Discussionmentioning

confidence: 99%

Optogenetic control of the Dab1 signaling pathway

Wang

Cooper

2017

Sci Rep

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The Reelin-Dab1 signaling pathway regulates development of the mammalian brain, including neuron migrations in various brain regions, as well as learning and memory in adults. Extracellular Reelin binds to cell surface receptors and activates phosphorylation of the intracellular Dab1 protein. Dab1 is required for most effects of Reelin, but Dab1-independent pathways may contribute. Here we developed a single-component, photoactivatable Dab1 (opto-Dab1) by using the blue light-sensitive dimerization/oligomerization property of A. thaliana Cryptochrome 2 (Cry2). Opto-Dab1 can activate downstream signals rapidly, locally, and reversibly upon blue light illumination. The high spatiotemporal resolution of the opto-Dab1 probe also allows us to control membrane protrusion, retraction and ruffling by local illumination in both COS7 cells and in primary neurons. This shows that Dab1 activation is sufficient to orient cell movement in the absence of other signals. Opto-Dab1 may be useful to study the biological functions of the Reelin-Dab1 signaling pathway both in vitro and in vivo.

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Section: Discussionmentioning

confidence: 99%

Optogenetic control of the Dab1 signaling pathway

Wang

Cooper

2017

Sci Rep

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“…Recent studies discovered RELN in peripheral organ systems as well. It has been found inter alia in human blood, liver, pancreas, mamma and intestines [22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

“…Alterations in RELN function are associated with several medical conditions in human beings. Amongst these are neuropsychiatric disorders such as schizophrenia, lissencephaly and Alzheimer's disease as well as a variety of other diseases, for instance cancerous diseases [24][25][26]. In schizophrenia for example, epigenetic changes lead to downregulation of cerebral RELN expression, while a mutation in the RELN gene is responsible for an autosomal recessive form of lissencephaly [27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Intra-day variations of blood reelin levels in healthy individuals

Sturm

Elst

Fiebich

et al. 2020

aoms

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Introduction: Reelin (RELN) is an extracellular glycoprotein best known to be crucial for neuronal migration during the embryonic period and regulation of synaptic plasticity in the adult brain, with recent studies pointing to reelin playing an important part in the organization of peripheral organs as well. Abnormalities in RELN function are associated with a variety of medical conditions in human beings. These alterations partly also reflect in RELN's blood levels, which gives it a clinical relevance as a potential biomarker. Requirement for a possible clinical use is a profound understanding of RELN's physiology. We hypothesized blood RELN levels could underlie time-dependent variations and therefore examined individuals' serum reelin concentrations in the course of one day. Material and methods: We obtained blood samples from healthy individuals (n = 10) at several times of measurement over a time period of 24 h. We subsequently determined the respective serum RELN concentrations utilizing an enzyme-linked immunosorbent assay and tested for intra-and interindividual variations in serum RELN concentrations over time. Results: All tested individuals' serum RELN levels displayed significant intraindividual variations in the course of 24 h. Test subjects' reelin day profiles showed substantial divergence among each other. Conclusions: Our findings point to short-term fluctuations in blood RELN levels being part of physiological RELN homeostasis. This is of special interest with regard to a potential clinical use of RELN as a biomarker.

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“…The earliest direct evidence was based on the postmortem studies of patients with SCZ, which revealed that RELN mRNA was reduced up to 50% in several regions of the brain [ 19 , 20 ]. Additionally, RELN had been shown to be expressed in organ systems, inter alia in human blood, liver, pancreas, breast and intestines [ 21 – 26 ]. A recent study determined that the patients with SCZ had higher level of RELN gene methylation compared to healthy controls, leading to a subsequent 25-fold decrease in RELN expression in the methylated group [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring the mRNA expression level of RELN in peripheral blood of schizophrenia patients before and after antipsychotic treatment

Yin

et al. 2020

Hereditas

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Background The Reelin (RELN) gene encodes the protein reelin, which is a large extracellular matrix glycoprotein that plays a key role in brain development. Additionally, this protein may be involved in memory formation, neurotransmission, and synaptic plasticity, which have been shown to be disrupted in schizophrenia (SCZ). A decreasing trend in the expression of RELN mRNA in the brain and peripheral blood of SCZ patients has been observed. There is a need to determine whether changes in RELN mRNA expression in SCZ patients are the result of long-term antipsychotic treatment rather than the etiological characteristics of schizophrenia. The expression levels of RELN mRNA in the peripheral blood of 48 healthy controls and 30 SCZ patients before and after 12-weeks of treatment were measured using quantitative real-time PCR. Results The expression levels of RELN mRNA in the SCZ group were significantly lower than that of healthy controls; however, after 12-weeks of antipsychotic treatment, RELN mRNA levels were significantly increased in the SCZ group. Conclusion The up-regulation of RELN mRNA expression was current in SCZ patients after antipsychotic treatment, suggesting that the changes in RELN mRNA expression were related to the effect of the antipsychotic treatment.

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Reelin‐Dab1 signaling system in human colorectal cancer

Cited by 22 publications

References 37 publications

Optogenetic control of the Dab1 signaling pathway

Optogenetic control of the Dab1 signaling pathway

Intra-day variations of blood reelin levels in healthy individuals

Exploring the mRNA expression level of RELN in peripheral blood of schizophrenia patients before and after antipsychotic treatment

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