Redundancy of washing in the preparation of biological specimens for transmission electron microscopy

Ockleford, Colin

doi:10.1111/j.1365-2818.1975.tb04050.x

Cited by 16 publications

(1 citation statement)

References 21 publications

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“…Transmission electron microscopy was carried out either as previously described (Ockleford 1975(Ockleford , 1976a, or on material fixed with 3 % (by mass) glutaraldehyde, followed by 2% osmium tetroxide, both in m phosphate buffer at pH 7.2, followed by viewing in a Jeol 100 CX electron microscope operated at 80 kV.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Morphogenesis of human placental chorionic villi: cytoskeletal, syncytioskeletal and extracellular matrix proteins

Ockleford

Wakely

Badley

1981

Proc. R. Soc. Lond. B.

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Immunocytochemical and ultrastructural methods were used to investigate the distribution of a family of structural proteins in the human placenta near term. These reveal the distribution of cytoskeletal and ‘syncytioskeletal’ components that may account for some of the more obvious micromorphological features of placental structure. In the syncytiotrophoblast a potentially supporting structure ‘the syncytioskeletal layer’ is described. It is an apparently continuous and complex polymeric network covering the villous tree, a surface of the order of 10m 2 in area in the full term placenta (Aherne & Dunnill 1966). It is suggested that this layer plays a part in morphogenesis of the villous tree.

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Section: Transmission Electron Microscopymentioning

confidence: 99%