Redundancy in putrescine catabolism in solvent tolerant Pseudomonas putida S12

Bandounas, Luaine; Ballerstedt, Hendrik; Winde, Johannes H. de; Ruijssenaars, Harald J.

doi:10.1016/j.jbiotec.2011.04.005

Cited by 12 publications

(10 citation statements)

References 51 publications

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“…The effect of putrescine on gene expression has been assessed in the highly homologous P. putida S12 using the P. putida KT2440 microarray. Among the 79 differentially regulated genes was a single chemoreceptor encoding gene, namely pp1228, which showed a 28-fold upregulation in the presence of putrescine (Bandounas et al, 2011). Here, we show that this gene encodes a chemoreceptor for putrescine and data thus indicate strong receptor induction in response to its cognate ligand.…”

Section: Discussionmentioning

confidence: 53%

“…Among the 79 differentially regulated genes was a single chemoreceptor encoding gene, namely pp1228, which showed a 28-fold upregulation in the presence of putrescine (Bandounas et al, 2011). Among the 79 differentially regulated genes was a single chemoreceptor encoding gene, namely pp1228, which showed a 28-fold upregulation in the presence of putrescine (Bandounas et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

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Assessment of the contribution of chemoreceptor‐based signalling to biofilm formation

Corral-Lugo

Torre

Matilla

et al. 2016

Environmental Microbiology

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Although it is well established that one- and two-component regulatory systems participate in regulating biofilm formation, there also exists evidence suggesting that chemosensory pathways are also involved. However, little information exists about which chemoreceptors and signals modulate this process. Here we report the generation of the complete set of chemoreceptor mutants of Pseudomonas putida KT2440 and the identification of four mutants with significantly altered biofilm phenotypes. These receptors are a WspA homologue of Pseudomonas aeruginosa, previously identified to control biofilm formation by regulating c-di-GMP levels, and three uncharacterized chemoreceptors. One of these receptors, named McpU, was found to mediate chemotaxis towards different polyamines. The functional annotation of McpU was initiated by high-throughput thermal shift assays of the receptor ligand binding domain (LBD). Isothermal titration calorimetry showed that McpU-LBD specifically binds putrescine, cadaverine and spermidine, indicating that McpU represents a novel chemoreceptor type. Another uncharacterized receptor, named McpA, specifically binds 12 different proteinogenic amino acids and mediates chemotaxis towards these compounds. We also show that mutants in McpU and WspA-Pp have a significantly reduced ability to colonize plant roots. Data agree with other reports showing that polyamines are signal molecules involved in the regulation of bacteria-plant communication and biofilm formation.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Assessment of the contribution of chemoreceptor‐based signalling to biofilm formation

Corral-Lugo

Torre

Matilla

et al. 2016

Environmental Microbiology

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“…In contrast, operons encoding metabolic pathways for 4-aminobutanoate (PP_2013-15), ethanolamine (PP_0542-44), and ornithine (PP_0999-1001) were strongly upregulated on 1,4-butanediol vs. glucose (Supplementary Data File S2). The metabolism of some of these amines shares metabolic intermediates with the putative 1,4-butanediol pathways (Bandounas et al, 2011). Possibly, the high accumulation of 4-hydroxybutyrate in the wildtype induced the expression of these genes, leading to a misregulation during growth on 1,4-butanediol.…”

Section: Systems Analysis Of 14-butanediol Degradation In P Putida mentioning

confidence: 99%

Unraveling 1,4-Butanediol Metabolism in Pseudomonas putida KT2440

Narancic

Kenny

et al. 2020

Front. Microbiol.

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“…The role of most of these genes in substrate catabolism has been validated in other Pseudomonas strains (65-71). Some of them, however, especially those related to putrescine catabolism, remain potential candidates for catabolic pathways (72). All of these genes represent interesting targets for a reverse genetics approach.…”

Section: Discussionmentioning

confidence: 99%

Metabolic and Genomic Traits of Phytobeneficial Phenazine-ProducingPseudomonasspp. Are Linked to Rhizosphere Colonization inArabidopsis thalianaandSolanum tuberosum

Zboralski

Biessy

Savoie

et al. 2020

Appl Environ Microbiol

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Bacterial rhizosphere colonization is critical for phytobeneficial rhizobacteria such as phenazine-producing Pseudomonas spp. To better understand this colonization process, potential metabolic and genomic determinants required for rhizosphere colonization were identified using a collection of 60 phenazine-producing Pseudomonas strains isolated from multiple plant species and representative of the worldwide diversity. Arabidopsis thaliana and Solanum tuberosum (potato) were used as host plants. Bacterial rhizosphere colonization was measured by quantitative PCR using a newly designed primer pair and TaqMan probe targeting a conserved region of the phenazine biosynthetic operon. The metabolic abilities of the strains were assessed on 758 substrates using Biolog phenotype microarray technology. These data, along with available genomic sequences for all strains, were analyzed in light of rhizosphere colonization. Strains belonging to the P. chlororaphis subgroup colonized the rhizospheres of both plants more efficiently than strains belonging to the P. fluorescens subgroup. Metabolic results indicated that the ability to use amines and amino acids was associated with an increase in rhizosphere colonization capability in A. thaliana and/or in S. tuberosum. The presence of multiple genetic determinants in the genomes of the different strains involved in catabolic pathways and plant-microbe and microbe-microbe interactions correlated with increased or decreased rhizosphere colonization capabilities in both plants. These results suggest that the metabolic and genomic traits found in different phenazine-producing Pseudomonas strains reflect their rhizosphere competence in A. thaliana and S. tuberosum. Interestingly, most of these traits are associated with similar rhizosphere colonizing capabilities in both plant species. IMPORTANCE Rhizosphere colonization is crucial for plant growth promotion and biocontrol by antibiotic-producing Pseudomonas spp. This colonization process relies on different bacterial determinants which partly remain to be uncovered. In this study, we combined a metabolic and a genomic approach to decipher new rhizosphere colonization determinants which could improve our understanding of this process in Pseudomonas spp. Using 60 distinct strains of phenazine-producing Pseudomonas spp., we show that rhizosphere colonization abilities correlated with both metabolic and genomic traits when these bacteria were inoculated on two distant plants, Arabidopsis thaliana and Solanum tuberosum. Key metabolic and genomic determinants presumably required for efficient colonization of both plant species were identified. Upon further validation, these targets could lead to the development of simple screening tests to rapidly identify efficient rhizosphere colonizers.

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Redundancy in putrescine catabolism in solvent tolerant Pseudomonas putida S12

Cited by 12 publications

References 51 publications

Assessment of the contribution of chemoreceptor‐based signalling to biofilm formation

Assessment of the contribution of chemoreceptor‐based signalling to biofilm formation

Unraveling 1,4-Butanediol Metabolism in Pseudomonas putida KT2440

Metabolic and Genomic Traits of Phytobeneficial Phenazine-ProducingPseudomonasspp. Are Linked to Rhizosphere Colonization inArabidopsis thalianaandSolanum tuberosum

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